Whoops 13/13 (sorry it’s been a long day and thread)

This spatial resolution captures cellular distributions of metals throughout the intestine which hasn’t been seen outside of synchrotron-XFM papers. With LA-ICP-MS systems more common than synchrotron beam lines I hope this method supports the community and C. elegans metal research. 12/12

With the TOF mass analyzer we can also visualize virtually every element on the periodic table simultaneously and provide rapid and comprehensive metal profile imaging of C. elegans. I optimized laser parameters to generate 2um spot size images. 12/X

Sorry, that’s the end of my vent thread. I’ll post when the manuscript is out. Spoiler alert: I developed a new way of preserving C. elegans without dehydration or fixatives for quantitative LA-ICP-TOF mass spec imaging to generate accurate metal images. 11/X

So I guess this manuscript will be a co-first author submission but the itemized author contributions will show how severely different our intellectual contributions are. I’ve never felt so helpless. I guess this is just a painful reminder that the academic system is broken. 10/X.

We’ve finally folded and admitted defeat. I wouldn’t have minded a co-first authorship with my friend even if the workload wasn’t equal but his PI invalidated my work during many of our arguments to support my friend’s co-first authorship and I feel so disrespected by a PI I thought was nice. 9/X

Because suddenly he felt the manuscript “wasn’t ready for submission” despite having sent us an email approving the manuscript content 24 hours earlier. It was clear he intended to delay submission again until they could generate more data/work to justify co-first authorship. 8/X

Which is a moot argument because that’s what PIs/corresponding authors do and we do not see every corresponding author listed as co-first authors. We then find out from the researcher that he was approached by his PI and was pressured to collect more data… 7/X

The worst part is that when we finally had an absolute timeline and intellectual merit/contributions presented to him, he began suggesting that the other researcher “contributed in intangible ways such as project direction guidance and mentorship” 6/X

By ignoring manuscript submission dates major deadlines for early career researchers such as ourselves. We’ve had 2 grants, an award and a fellowship that would have benefited from this manuscript being submitted. 5/X

The collaborating PI has been fighting tooth and nail and pressuring us to give them a cosmetic co-first authorship without intellectual merit for awhile now. They are a well established PI at our university and have disrespected our small and new lab at every opportunity 4/X

I’ve given my supposedly co-first author real-time updates on my work because he’s a friend and still on the manuscript in some capacity. However, since I’ve pulled ahead in terms of intellectual merit significantly that meant we needed to re-evaluate authorship. 3/X

The manuscript was originally intended to be a co-first author situation, however, my colleague stopped working on the manuscript about 1.5 years ago and that forced me to finish the work. Since he stopped, I generated so much data that this new manuscript draft is 90% of my data now. 2/X

This is so helpful, thank you!

C. elegans! We decided to transfer them to a colleague’s -80 and hope they revive. Another C. elegans researcher said as long as they don’t thaw they should be okay but we’re still really concerned.

This is our first time with worms in this situation so we’re really in the dark. I appreciate any advice for how to handle worm frozen stocks when temperatures shift this much!

Woohoo! That’s my PI (and that will be some of my data presented)! 😆

Now i need to hurry up and publish my imaging results soon, too 🤣