Abhinay Ramaprasad
@abhinay.bsky.social
Malaria biologist. Can stitch together some code. MRC Career Development Fellow at School of Infection and Immunity, UofGlasgow @uofgsii.bsky.social. #NewPI. Fuelled by chai. www.parafugelab.com
Coming back from vacation to find that I have been replaced. Lab peeps seemed to have midipreped a new PI.
November 26, 2025 at 2:38 PM
Coming back from vacation to find that I have been replaced. Lab peeps seemed to have midipreped a new PI.
GRiP-ing talk tomorrow from Natalia de Miguel from Instituto Tecnológico de Chascomús (INTECH), Buenos Aires, Argentina. Join us!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link: uofglasgow.zoom.us/j/8674596707...
Passcode: 262806
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link: uofglasgow.zoom.us/j/8674596707...
Passcode: 262806
May 1, 2025 at 3:57 PM
GRiP-ing talk tomorrow from Natalia de Miguel from Instituto Tecnológico de Chascomús (INTECH), Buenos Aires, Argentina. Join us!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link: uofglasgow.zoom.us/j/8674596707...
Passcode: 262806
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link: uofglasgow.zoom.us/j/8674596707...
Passcode: 262806
Sorry if you are facing issues, try entering this meeting id
April 25, 2025 at 1:13 PM
Sorry if you are facing issues, try entering this meeting id
GRiP-ing Crypto talk tomorrow from Mattie Pawlowic from the University of Dundee. Join us!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link- uofglasgow.zoom.us/j/8172068584...
Passcode: 038661
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link- uofglasgow.zoom.us/j/8172068584...
Passcode: 038661
April 24, 2025 at 1:40 PM
GRiP-ing Crypto talk tomorrow from Mattie Pawlowic from the University of Dundee. Join us!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link- uofglasgow.zoom.us/j/8172068584...
Passcode: 038661
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link- uofglasgow.zoom.us/j/8172068584...
Passcode: 038661
A GRIP-ing and Tryp-ing seminar tomorrow - Joana Faria from the University of York!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link - uofglasgow.zoom.us/j/8897218018...
Passcode: 987702
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link - uofglasgow.zoom.us/j/8897218018...
Passcode: 987702
April 10, 2025 at 1:23 PM
A GRIP-ing and Tryp-ing seminar tomorrow - Joana Faria from the University of York!
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link - uofglasgow.zoom.us/j/8897218018...
Passcode: 987702
2 pm (GMT) | 3 pm (CET) | 9 am (EDT)
Link - uofglasgow.zoom.us/j/8897218018...
Passcode: 987702
GRIP-ing seminar this Friday from André Schneider from University of Bern. Don't miss it!
Link: uofglasgow.zoom.us/j/8255427338...
Passcode: 984619
Link: uofglasgow.zoom.us/j/8255427338...
Passcode: 984619
March 26, 2025 at 11:44 AM
GRIP-ing seminar this Friday from André Schneider from University of Bern. Don't miss it!
Link: uofglasgow.zoom.us/j/8255427338...
Passcode: 984619
Link: uofglasgow.zoom.us/j/8255427338...
Passcode: 984619
"It's about time" for another GRiPing talk this Friday! We are delighted to have Sarah Reece @reecelab.bsky.social
from @edinburgh-uni.bsky.social
Link: uofglasgow.zoom.us/j/8825975465...
Passcode: 186275
from @edinburgh-uni.bsky.social
Link: uofglasgow.zoom.us/j/8825975465...
Passcode: 186275
March 12, 2025 at 5:54 PM
"It's about time" for another GRiPing talk this Friday! We are delighted to have Sarah Reece @reecelab.bsky.social
from @edinburgh-uni.bsky.social
Link: uofglasgow.zoom.us/j/8825975465...
Passcode: 186275
from @edinburgh-uni.bsky.social
Link: uofglasgow.zoom.us/j/8825975465...
Passcode: 186275
GRiP-ing seminars tomorrow! Our speaker for the day is Cecile Crosnier from University of York. Come, join us!
February 27, 2025 at 3:13 PM
GRiP-ing seminars tomorrow! Our speaker for the day is Cecile Crosnier from University of York. Come, join us!
GRiP-ing seminars continue this Friday! Our next speaker is Emma Wilson @toxobrains.bsky.social from @ucriverside.bsky.social. Come, join us!
Link: uofglasgow.zoom.us/j/8640933246...
Passcode: 719067
Link: uofglasgow.zoom.us/j/8640933246...
Passcode: 719067
February 18, 2025 at 11:42 AM
GRiP-ing seminars continue this Friday! Our next speaker is Emma Wilson @toxobrains.bsky.social from @ucriverside.bsky.social. Come, join us!
Link: uofglasgow.zoom.us/j/8640933246...
Passcode: 719067
Link: uofglasgow.zoom.us/j/8640933246...
Passcode: 719067
GRiP-ing seminar this Friday! Our speaker is Paul Sigala from @utah.edu.
Come, join us!
Link: uofglasgow.zoom.us/j/8847101592...
Passcode: 896279
Come, join us!
Link: uofglasgow.zoom.us/j/8847101592...
Passcode: 896279
February 12, 2025 at 8:52 PM
GRiP-ing seminar this Friday! Our speaker is Paul Sigala from @utah.edu.
Come, join us!
Link: uofglasgow.zoom.us/j/8847101592...
Passcode: 896279
Come, join us!
Link: uofglasgow.zoom.us/j/8847101592...
Passcode: 896279
Newsworthy aside, graphical abstract game is strong with this one - www.nytimes.com/2025/02/11/u...
February 11, 2025 at 8:06 PM
Newsworthy aside, graphical abstract game is strong with this one - www.nytimes.com/2025/02/11/u...
The new Lonza model looks yum. Costs three legs, two arms and a thumb.. but looks yum. No more eternally collapsing display.
January 22, 2025 at 11:00 AM
The new Lonza model looks yum. Costs three legs, two arms and a thumb.. but looks yum. No more eternally collapsing display.
GRiP-ing seminars, 2025 edition starts next Friday, live from Glasgow! Our first speaker is Emma Wilson @toxobrains.bsky.social from @ucriverside.bsky.social.
Come, join us!
Please RT. DM to get mail alerts about these excellent talks.
Link: uofglasgow.zoom.us/j/8894673417...
Passcode: 410932
Come, join us!
Please RT. DM to get mail alerts about these excellent talks.
Link: uofglasgow.zoom.us/j/8894673417...
Passcode: 410932
January 17, 2025 at 4:51 PM
GRiP-ing seminars, 2025 edition starts next Friday, live from Glasgow! Our first speaker is Emma Wilson @toxobrains.bsky.social from @ucriverside.bsky.social.
Come, join us!
Please RT. DM to get mail alerts about these excellent talks.
Link: uofglasgow.zoom.us/j/8894673417...
Passcode: 410932
Come, join us!
Please RT. DM to get mail alerts about these excellent talks.
Link: uofglasgow.zoom.us/j/8894673417...
Passcode: 410932
With Anagha's help, we have started some exciting molbio work and resurrecting malaria parasites out of the deep freeze! They seem to be cozy and happy in their incubator as we at Glasgow enter the deep freeze..
January 10, 2025 at 11:42 AM
With Anagha's help, we have started some exciting molbio work and resurrecting malaria parasites out of the deep freeze! They seem to be cozy and happy in their incubator as we at Glasgow enter the deep freeze..
We welcomed our first ParaFuGer - Anagha Rajesh Salvi as the dependable lab technician.
January 10, 2025 at 11:42 AM
We welcomed our first ParaFuGer - Anagha Rajesh Salvi as the dependable lab technician.
The lab is 4-months old (awwww). Time for some updates!
We have a name - The ParaFuGe Lab!
What, no we don't do waterproofing of the facade and bricks, stop calling us
We have a name - The ParaFuGe Lab!
What, no we don't do waterproofing of the facade and bricks, stop calling us
January 10, 2025 at 11:42 AM
The lab is 4-months old (awwww). Time for some updates!
We have a name - The ParaFuGe Lab!
What, no we don't do waterproofing of the facade and bricks, stop calling us
We have a name - The ParaFuGe Lab!
What, no we don't do waterproofing of the facade and bricks, stop calling us
With just 7 timepoints, our streamlined SHIFTiKO workflow makes it easier to screen small groups of essential genes to quickly weed out false "essential" genes, identify genes with a particular phenotype, and produce a resource of shiftiko lines for more in-depth follow-up.
January 1, 2025 at 6:30 PM
With just 7 timepoints, our streamlined SHIFTiKO workflow makes it easier to screen small groups of essential genes to quickly weed out false "essential" genes, identify genes with a particular phenotype, and produce a resource of shiftiko lines for more in-depth follow-up.
With malarial rhomboid proteases as examples, we show how SHIFTiKO lets us quickly screen if genes have been primed for excision, successfully excised, whether mutants can grow, and if not, what kind of defect stops them.
January 1, 2025 at 6:30 PM
With malarial rhomboid proteases as examples, we show how SHIFTiKO lets us quickly screen if genes have been primed for excision, successfully excised, whether mutants can grow, and if not, what kind of defect stops them.
So we made several tweaks, majorly two - i) inducibly excise only a small region in the gene such that it results in a frameshift mutation and thereby knock out and ii) tag with DNA barcodes such that mutants can be quantified in a pool using barcode-sequencing.
January 1, 2025 at 6:30 PM
So we made several tweaks, majorly two - i) inducibly excise only a small region in the gene such that it results in a frameshift mutation and thereby knock out and ii) tag with DNA barcodes such that mutants can be quantified in a pool using barcode-sequencing.
DiCre is awesome for studying one gene at a time but tackling multiple genes soon starts to get expensive, time-consuming and exhaustive. I faced this issue as a postdoc wanting to study several enzymes but also wanting to leave the lab at a reasonable hour.
January 1, 2025 at 6:30 PM
DiCre is awesome for studying one gene at a time but tackling multiple genes soon starts to get expensive, time-consuming and exhaustive. I faced this issue as a postdoc wanting to study several enzymes but also wanting to leave the lab at a reasonable hour.
This is where inducible tools like the DiCre-lox system help. We can prime a gene for deletion (by flanking it with loxP sites), trigger the deletion in a population of parasites with a drug (rapamycin) and study the ensuing defect within a single replication cycle.
January 1, 2025 at 6:30 PM
This is where inducible tools like the DiCre-lox system help. We can prime a gene for deletion (by flanking it with loxP sites), trigger the deletion in a population of parasites with a drug (rapamycin) and study the ensuing defect within a single replication cycle.
Woohoo! Parasitaemia has reached 100 with @princeubiaru.bsky.social entering the fold!
November 17, 2024 at 7:05 PM
Woohoo! Parasitaemia has reached 100 with @princeubiaru.bsky.social entering the fold!
Weird reagents ordering system in the dept where one has to fill and send an excel form (one per supplier) and quote by email. Made an Automate script that reads from the lab's master list, fill the forms, name files properly, and email them. No to filling forms! #labedit #newPI
November 11, 2024 at 9:18 PM
Can't gif?? Noooooo
December 30, 2023 at 9:23 AM
Can't gif?? Noooooo