Mark Williams single molecule biophysics lab
@williamslabneu.bsky.social
Optical tweezers | AFM | correlated fluorescence and force | nucleic acid-protein interactions | SSBs | chromatin stability | nucleosome chaperones | retrovirus, coronavirus, retrotransposon replication
https://williamslab.sites.northeastern.edu
https://williamslab.sites.northeastern.edu
We measured single molecule ssDNA binding by SSB proteins from different systems, E. coli SSB, T4 gene 32, and LINE-1 ORF1p. All showed surprising initial compaction then decompaction. This allows them to dissociate rapidly in response to crowding, as we show in QRB Discovery doi.org/10.1017/qrd....
Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function | QRB Discovery | Cambridge Core
Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function - Volume 6
doi.org
January 22, 2025 at 11:05 PM
We measured single molecule ssDNA binding by SSB proteins from different systems, E. coli SSB, T4 gene 32, and LINE-1 ORF1p. All showed surprising initial compaction then decompaction. This allows them to dissociate rapidly in response to crowding, as we show in QRB Discovery doi.org/10.1017/qrd....
When B DNA is overstretched, it converts to melted DNA or S-DNA. Overstretching has been controversial because the transitions are similar for the two states. With the Westerlund and Wilhelmsson labs, we show in NAR the B-to-S transition is much faster than melting
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics
Abstract. It is widely appreciated that double stranded DNA (dsDNA) is subjected to strong and dynamic mechanical forces in cells. Under increasing tension
academic.oup.com
January 18, 2025 at 5:17 PM
When B DNA is overstretched, it converts to melted DNA or S-DNA. Overstretching has been controversial because the transitions are similar for the two states. With the Westerlund and Wilhelmsson labs, we show in NAR the B-to-S transition is much faster than melting
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
How does the ORF1p perform its multiple functions to facilitate LINE1 retrotransposition? And what enables its ability to also facilitate replication of other retrotransposons? Find out in our newly published paper in Nucleic Acids Research doi.org/10.1093/nar/...
L1-ORF1p nucleoprotein can rapidly assume distinct conformations and simultaneously bind more than one nucleic acid
Abstract. LINE-1 (L1) is a parasitic retrotransposable DNA element, active in primates for the last 80–120 Myr. L1 has generated nearly one-third of the hu
doi.org
December 23, 2024 at 2:57 AM
How does the ORF1p perform its multiple functions to facilitate LINE1 retrotransposition? And what enables its ability to also facilitate replication of other retrotransposons? Find out in our newly published paper in Nucleic Acids Research doi.org/10.1093/nar/...