Fanny DEMAY
@titefanny.bsky.social
Postdoc in BalikovaNovotna Lab, Institute of Microbiology, Czech Academy of Science, Prague
#ribosome #translation #antibiotiques #c. difficile #staph. aureus #ABCF proteins #Methyltransferase
#ribosome #translation #antibiotiques #c. difficile #staph. aureus #ABCF proteins #Methyltransferase
Reposted by Fanny DEMAY
🦠 Is life in the universe rare, or might it occur almost everywhere?
The PROTOCELL project by @hlouchova-lab.bsky.social in collab with @pluskal-lab.org and with support of @esa.int aims to find out ► www.uochb.cz/en/news/742/...
#IOCB #IOCBPrague #AVCR #chemie #chemistry #czechscience
The PROTOCELL project by @hlouchova-lab.bsky.social in collab with @pluskal-lab.org and with support of @esa.int aims to find out ► www.uochb.cz/en/news/742/...
#IOCB #IOCBPrague #AVCR #chemie #chemistry #czechscience
November 24, 2025 at 12:57 PM
🦠 Is life in the universe rare, or might it occur almost everywhere?
The PROTOCELL project by @hlouchova-lab.bsky.social in collab with @pluskal-lab.org and with support of @esa.int aims to find out ► www.uochb.cz/en/news/742/...
#IOCB #IOCBPrague #AVCR #chemie #chemistry #czechscience
The PROTOCELL project by @hlouchova-lab.bsky.social in collab with @pluskal-lab.org and with support of @esa.int aims to find out ► www.uochb.cz/en/news/742/...
#IOCB #IOCBPrague #AVCR #chemie #chemistry #czechscience
Reposted by Fanny DEMAY
‼️🚨🗓️ CONTAMCALENDAR 2026 🗓️🚨‼️
Excited to say the ContamCalendar is back for its fifth edition! We’ve created a fresh new design, folding out for an A3 monthly spread. It’s our best yet, we hope you’ll love it 🗓️🦠🧫🍄
Get yours here ➡️ www.contam.club ⬅️
Excited to say the ContamCalendar is back for its fifth edition! We’ve created a fresh new design, folding out for an A3 monthly spread. It’s our best yet, we hope you’ll love it 🗓️🦠🧫🍄
Get yours here ➡️ www.contam.club ⬅️
November 21, 2025 at 3:30 PM
‼️🚨🗓️ CONTAMCALENDAR 2026 🗓️🚨‼️
Excited to say the ContamCalendar is back for its fifth edition! We’ve created a fresh new design, folding out for an A3 monthly spread. It’s our best yet, we hope you’ll love it 🗓️🦠🧫🍄
Get yours here ➡️ www.contam.club ⬅️
Excited to say the ContamCalendar is back for its fifth edition! We’ve created a fresh new design, folding out for an A3 monthly spread. It’s our best yet, we hope you’ll love it 🗓️🦠🧫🍄
Get yours here ➡️ www.contam.club ⬅️
Reposted by Fanny DEMAY
🚨Attention prospective PhD students🚨
Are you interested in molecular mechanisms, RNA viruses, structural biology or biophysics? 🧬🦠🧪
We have THREE fully-funded PhD projects available for October 2026 entry:
www.findaphd.com/phds/?Keywor...
Please share/repost, and get in touch if you're interested
Are you interested in molecular mechanisms, RNA viruses, structural biology or biophysics? 🧬🦠🧪
We have THREE fully-funded PhD projects available for October 2026 entry:
www.findaphd.com/phds/?Keywor...
Please share/repost, and get in touch if you're interested
chris hill PhD Projects, Programmes & Scholarships - 3 PhDs Listed
FindAPhD. Search Funded PhD Projects, Programmes & Scholarships in chris hill. Search for PhD funding, scholarships & studentships in the UK, Europe and around the world.
www.findaphd.com
November 13, 2025 at 7:54 PM
🚨Attention prospective PhD students🚨
Are you interested in molecular mechanisms, RNA viruses, structural biology or biophysics? 🧬🦠🧪
We have THREE fully-funded PhD projects available for October 2026 entry:
www.findaphd.com/phds/?Keywor...
Please share/repost, and get in touch if you're interested
Are you interested in molecular mechanisms, RNA viruses, structural biology or biophysics? 🧬🦠🧪
We have THREE fully-funded PhD projects available for October 2026 entry:
www.findaphd.com/phds/?Keywor...
Please share/repost, and get in touch if you're interested
Reposted by Fanny DEMAY
Many nice #RNA & #ribosome papers in the latest issue of @narjournal.bsky.social 🦠💫 academic.oup.com/nar/issue/53...
⬇️links in comments
⬇️links in comments
November 12, 2025 at 7:58 PM
Many nice #RNA & #ribosome papers in the latest issue of @narjournal.bsky.social 🦠💫 academic.oup.com/nar/issue/53...
⬇️links in comments
⬇️links in comments
Reposted by Fanny DEMAY
EF-G variants (fusA mutants) slow down ribosome translocation only when the ribosome is bound by an aminoglycoside => the antibiotic isn’t displaced, but translation on that ribosome is effectively stalled 🦠 rdcu.be/eNt96
Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides
Nature Communications - Mutations in elongation factor G protect bacteria from aminoglycoside antibiotics through unknown mechanisms. Here, the authors show that the mutations selectively slow the...
rdcu.be
October 30, 2025 at 1:10 PM
EF-G variants (fusA mutants) slow down ribosome translocation only when the ribosome is bound by an aminoglycoside => the antibiotic isn’t displaced, but translation on that ribosome is effectively stalled 🦠 rdcu.be/eNt96
Reposted by Fanny DEMAY
We are hiring…position suitable for PhD student with interest in ribosomes, antibiotics and cryoEM…
www.uni-hamburg.de/en/stellenan...
www.uni-hamburg.de/en/stellenan...
Job advertisement
www.uni-hamburg.de
October 16, 2025 at 4:02 PM
We are hiring…position suitable for PhD student with interest in ribosomes, antibiotics and cryoEM…
www.uni-hamburg.de/en/stellenan...
www.uni-hamburg.de/en/stellenan...
Reposted by Fanny DEMAY
Colliding ribosomes are potent signals of cellular stress. But do cells use ‘programmed’ ribosome collisions to regulate gene expression? I’m excited to present a new story from my lab led by Frederick Rehfeld(@fred-rehfeld.bsky.social) which revealed that the answer is YES! Read on to find out how👇
Oxidative stress sensing by the translation elongation machinery promotes production of detoxifying selenoproteins
Selenocysteine, incorporated into polypeptides at recoded termination codons, plays an essential role in redox biology. Using GPX1 and GPX4, selenoenzymes that mitigate oxidative stress, as reporters,...
www.biorxiv.org
October 14, 2025 at 10:28 PM
Colliding ribosomes are potent signals of cellular stress. But do cells use ‘programmed’ ribosome collisions to regulate gene expression? I’m excited to present a new story from my lab led by Frederick Rehfeld(@fred-rehfeld.bsky.social) which revealed that the answer is YES! Read on to find out how👇
Reposted by Fanny DEMAY
Our method paper to produce efficient cell-free translation lysates from diverse human cell types is out 🎉 www.jbc.org/article/S002... @unibe.ch @mcid-unibe.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
May 28, 2025 at 4:13 PM
Our method paper to produce efficient cell-free translation lysates from diverse human cell types is out 🎉 www.jbc.org/article/S002... @unibe.ch @mcid-unibe.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
Reposted by Fanny DEMAY
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal peptidyl transferase inhibitor
Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...
www.biorxiv.org
October 13, 2025 at 10:35 AM
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
Reposted by Fanny DEMAY
Mycobacterial TrmI (m¹A58) is linked to aminoglycoside tolerance. Ribosomes stall in an alanine-rich region that controls whiB7, and trans-translation increases this stalling, keeping WhiB7 active and helping mycobacteria survive antibiotic stress.🦠#rnasky => preprint www.biorxiv.org/content/10.1...
tRNA-modification mutants in mycobacteria boost antibiotic recalcitrance by activating WhiB7 & coupling alanine metabolism to ribosome rescue and survival, new study reveals.
✍️ @jvaubourgeix.bsky.social & coll.
📖 shorturl.at/86JTs
#MicroSky #AMR #RNAsky
@imperialcollegeldn.bsky.social @inserm.fr
✍️ @jvaubourgeix.bsky.social & coll.
📖 shorturl.at/86JTs
#MicroSky #AMR #RNAsky
@imperialcollegeldn.bsky.social @inserm.fr
October 13, 2025 at 7:29 AM
Mycobacterial TrmI (m¹A58) is linked to aminoglycoside tolerance. Ribosomes stall in an alanine-rich region that controls whiB7, and trans-translation increases this stalling, keeping WhiB7 active and helping mycobacteria survive antibiotic stress.🦠#rnasky => preprint www.biorxiv.org/content/10.1...
Reposted by Fanny DEMAY
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal peptidyl transferase inhibitor
Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...
www.biorxiv.org
October 13, 2025 at 12:24 PM
Reposted by Fanny DEMAY
Dvě vědkyně z @mbuavcr.bsky.social - Natalia Slonská a Lucie Najmanová, se zúčastnily soutěže Věda fotogenická, kterou pořádá @akademievedcr.bsky.social. Do soutěže přihlásily snímky, které propojují krásu květu fuchsie se světem půdních bakterií Streptomyces coelicolor.
August 29, 2025 at 9:32 AM
Dvě vědkyně z @mbuavcr.bsky.social - Natalia Slonská a Lucie Najmanová, se zúčastnily soutěže Věda fotogenická, kterou pořádá @akademievedcr.bsky.social. Do soutěže přihlásily snímky, které propojují krásu květu fuchsie se světem půdních bakterií Streptomyces coelicolor.
Reposted by Fanny DEMAY
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
September 29, 2025 at 3:01 PM
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
Reposted by Fanny DEMAY
Structural insights into YheS-mediated release of SecM-arrested ribosome. www.biorxiv.org/content/10.1... 🦠 #ribosome #rnasky
Structural insights into YheS-mediated release of SecM-arrested ribosome.
ATPase-binding cassette subfamily F (ABCF) proteins interact with the ribosome to resolve translation defects near the peptidyl-transferase center, induced by ribosome-targeting antibiotics or "hard-t...
www.biorxiv.org
August 31, 2025 at 11:51 AM
Structural insights into YheS-mediated release of SecM-arrested ribosome. www.biorxiv.org/content/10.1... 🦠 #ribosome #rnasky
Reposted by Fanny DEMAY
Here, we show that bacterial ribosomes—often viewed as conserved drug targets—actually vary across species due to extensive sequence variation in their drug-binding residues. In some cases, this divergence may lead to intrinsic drug resistance in certain bacterial clades: doi.org/10.1016/j.ce...
June 19, 2025 at 7:32 AM
Here, we show that bacterial ribosomes—often viewed as conserved drug targets—actually vary across species due to extensive sequence variation in their drug-binding residues. In some cases, this divergence may lead to intrinsic drug resistance in certain bacterial clades: doi.org/10.1016/j.ce...
Reposted by Fanny DEMAY
Happy to share a new paper from our lab with my little contribution! 🥳🥳🥳
We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️
We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️
New ABCF article from @balikovalab.bsky.social group!
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
ABCF protein-mediated resistance shapes bacterial responses to antibiotics based on their type and concentration | mBio
Bacteria adapt to diverse stimuli mainly through transcriptional changes that regulate adaptive protein factors. Here, we show that responses to protein synthesis-inhibiting antibiotics are fine-tuned by antibiotic resistance ABCF proteins at the ...
doi.org
August 13, 2025 at 11:54 AM
Happy to share a new paper from our lab with my little contribution! 🥳🥳🥳
We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️
We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️
New ABCF article from @balikovalab.bsky.social group!
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
ABCF protein-mediated resistance shapes bacterial responses to antibiotics based on their type and concentration | mBio
Bacteria adapt to diverse stimuli mainly through transcriptional changes that regulate adaptive protein factors. Here, we show that responses to protein synthesis-inhibiting antibiotics are fine-tuned by antibiotic resistance ABCF proteins at the ...
doi.org
August 13, 2025 at 11:23 AM
New ABCF article from @balikovalab.bsky.social group!
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
Even though ABCF proteins have the same shape and act on the ribosome E-site, they have very different role in bacteria physiology level.
What an interesting topic!🤩
doi.org/10.1128/mbio...
Reposted by Fanny DEMAY
Editor's Pick: Shen, Ye et al. review recent literature on the role of bacterial ribosome heterogeneity on facilitating rapid response to stress.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
July 27, 2025 at 1:55 PM
Reposted by Fanny DEMAY
''natural human tRNAAla variants generate mistranslation leading to defects in protein production that depend on the nature of the amino acid replacement.'' #rnasky Natural human tRNAAla anticodon variants mistranslate the genetic code
m.rnajournal.cshlp.org/content/earl...
m.rnajournal.cshlp.org/content/earl...
RNA | Mobile
m.rnajournal.cshlp.org
July 17, 2025 at 6:27 AM
''natural human tRNAAla variants generate mistranslation leading to defects in protein production that depend on the nature of the amino acid replacement.'' #rnasky Natural human tRNAAla anticodon variants mistranslate the genetic code
m.rnajournal.cshlp.org/content/earl...
m.rnajournal.cshlp.org/content/earl...
Reposted by Fanny DEMAY
Preprint from our collaboration with the labs of Gerry Wright and Shura Mankin identifying the first translation inhibitor binding to the E-site of the bacterial ribosome… www.researchsquare.com/article/rs-6...
A Natural Depsipeptide Antibiotic that Targets the E site of the Bacterial Ribosome
A significant challenge in addressing the antibiotic resistance crisis is identifying new antimicrobial compounds. Although natural products produced by fungi and bacteria, particularly actinomycetes,...
www.researchsquare.com
July 14, 2025 at 11:29 AM
Preprint from our collaboration with the labs of Gerry Wright and Shura Mankin identifying the first translation inhibitor binding to the E-site of the bacterial ribosome… www.researchsquare.com/article/rs-6...
Reposted by Fanny DEMAY
Insights on how ribosomal RNA modification distant from drug binding site can still affect drug binding, in mycobacterium 🦠 #rnasky #ribosome #microsky
Distant ribose 2'-O-methylation of 23S rRNA helix 69 pre-orders the capreomycin drug binding pocket at the ribosome subunit interface pubmed.ncbi.nlm.nih.gov/40626557/ #cryoem
July 10, 2025 at 7:33 AM
Bye Bye "Ribosome2025", Pacific Grove, California!🌁🌊
It was a very nice conference with beautiful science and amazing people!!🤩👌
It was a very nice conference with beautiful science and amazing people!!🤩👌
June 29, 2025 at 1:47 AM
Bye Bye "Ribosome2025", Pacific Grove, California!🌁🌊
It was a very nice conference with beautiful science and amazing people!!🤩👌
It was a very nice conference with beautiful science and amazing people!!🤩👌
Reposted by Fanny DEMAY
Paper alert: www.sciencedirect.com/science/arti...? Proud that we brought together researchers, clinicians and patient/carers to convey our point that it is important to bring novel treatments for bacterial pathogens to patients *also* if they are not on the WHO Bacterial Priority Pathogen list.
Clostridioides difficile should be considered a bacterial priority pathogen
www.sciencedirect.com
June 26, 2025 at 12:48 PM
Paper alert: www.sciencedirect.com/science/arti...? Proud that we brought together researchers, clinicians and patient/carers to convey our point that it is important to bring novel treatments for bacterial pathogens to patients *also* if they are not on the WHO Bacterial Priority Pathogen list.
Reposted by Fanny DEMAY
Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome www.science.org/doi/10.1126/...
Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome
Translation termination is essential in all living organisms because it ensures that proteins have lengths strictly defined by their genes. This universal process is mediated by peptide release factor...
www.science.org
June 19, 2025 at 7:39 PM
Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome www.science.org/doi/10.1126/...