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Dan Kliebenstein @spicybotrytis.bsky.social · 4h

I wonder if the glucosinolate pathway also responds to catechol like some other plant genes. The non-host specific component would suggest that likely so. onlinelibrary.wiley.com/doi/full/10....

I wonder if Agro has a nahG? To the genome browser.

Loss of non‐host resistance of Arabidopsis NahG to Pseudomonas syringae pv. phaseolicola is due to degradation products of salicylic acid

In plants carrying the NahG transgene, salicylate hydroxylase converts salicylic acid (SA) to catechol. Arabidopsis NahG plants are defective in non-host resistance to Pseudomonas syringae pv. phaseo....

onlinelibrary.wiley.com

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Dan Kliebenstein @spicybotrytis.bsky.social · 4h

Just might work. Need to get an exogenous SA inhibitor to co-infiltrate with and it might just work. Or maybe co-infiltrate with coronatine?

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Dan Kliebenstein @spicybotrytis.bsky.social · 5h

And making sure the plant doesn't respond to the Agro. For some reason, infiltration of Arabidopsis with Agro shuts down endogenous glucosinolate biosynthesis. Had hoped to do transient complementations but no such luck.

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Dan Kliebenstein @spicybotrytis.bsky.social · 1d

a picture of a cartoon character with the word goodnight below him

ALT: a picture of a cartoon character with the word goodnight below him

media.tenor.com

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Dan Kliebenstein @spicybotrytis.bsky.social · 1d

Maybe needed my grandmothers antique giant ceramic crock pot? Big enough for the largest cabbage leaf.

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Dan Kliebenstein @spicybotrytis.bsky.social · 1d

Make it ABA inducible so color tracks watering?

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Reposted by Dan Kliebenstein

Alejandro Montenegro @aemonten.bsky.social · 3d

What makes me really happy is that someone as experienced as yourself continues to see the value of professional editors in helping improve the writing :)

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Dan Kliebenstein @spicybotrytis.bsky.social · 3d

Half of this years squash crop. All volunteer due to megafauna dispersal.

Squash harvested from seeds planted by mastiff

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

I feel like the key is this "reduced support with the perception that it is ‘done’,".

It is so weird to think that the community can think that an organism with maybe 1000 genes out of 20,000+ having been studied to the level of one or two papers is "Done".

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

After having @planteditors.bsky.social look through a paper and only suggest a single wording change within the main text. Only took 24 years to get there. (Gotta love that caveat)

a dachshund wearing a cowboy hat and a blue shirt says party time

ALT: a dachshund wearing a cowboy hat and a blue shirt says party time

media.tenor.com

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

I feel like that title should be “a plant pathogens advance”.

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

I am not sure that reviewers really dive into the logic of ideas over their feel.

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

Systems of systems get even nuttier.

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Dan Kliebenstein @spicybotrytis.bsky.social · 4d

Host-pathogen cotranscriptome papers are really intricate to write as you have genes that are host influenced, pathogen influenced and interaction influenced in both the host and the pathogen.

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Dan Kliebenstein @spicybotrytis.bsky.social · 5d

I promise the low monotone makes it the perfect recording to induce sleep.

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Reposted by Dan Kliebenstein

Marc Somssich @somssich.bsky.social · 5d

Our article "Guns in Rosettes: The Arabidopsis chemical weapons arsenal" is finally published @plantphys.bsky.social!
You can download the #OpenAccess PDF here:

doi.org/10.1093/plph...

But real connoisseurs of #AntimicrobialCompounds will want to get the vinyl boxset: #PlantScience #PlantImmunity

A vinyl box with six colored vinyl LPs sticking out of its right side. The Title "Guns in Rosettes" is on top, with the subtitle "The Arabidopsis chemical weapons arsenal" just below. Then there is an Arabidopsis plant with machine guns sticking out. Below is the statement "6-LP LIMITED EDITION COLORED VINYL BOX SET"

The back cover of the vinyl box set showing the tracklist:
1. Methionine-derived compounds
Aliphatic GSLs
2. Tryptophan-derived compounds (trptonites)
Indolic GSLs
Camalexin
Hycanite (4-OH-ICN)
3. Phenylalanine-derived compounds (phenylpropanoids)
Benzenic GSLs
Coumarins
Hydroxycinnamates
Flavonoids
4. Terpenoids
(E)-β-caryophyllene
α- and β-pinene
Thalianin, arabidin, and DMNT

And the statement "Recorded live in front of an audience in 2025"

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Dan Kliebenstein @spicybotrytis.bsky.social · 5d

Statistics has never been about coding, it is about experimental design, appropriate application and interpretation.

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Dan Kliebenstein @spicybotrytis.bsky.social · 6d

You would be surprised how many assume plants only have fight options. The idea of over-compensation has very little saturation in the molecular community interested in growth vs defense tradeoffs.

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Dan Kliebenstein @spicybotrytis.bsky.social · 6d

I am wondering if it is more 50/50 for primary but specialized rapidly collapse into primary catabolism. Quicker than synthesis diverges.

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Dan Kliebenstein @spicybotrytis.bsky.social · 6d

In response to herbivory they can fight, evade or even over compensate. The later two are development as defense.

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Dan Kliebenstein @spicybotrytis.bsky.social · 7d

That is kind of my gut. How would you split Anabolism vs catabolism? And how does energy differ across CNSP? Or is that super lineage specific?

As in Barley with 20% cyanogen dry weight is just wack.

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Dan Kliebenstein @spicybotrytis.bsky.social · 7d

Thinking of the standard senntence saying “because plants are sessile they …”. Is the direction arrow correct? Or is sessile not a cause but a consequence?

I.e. because plants are autotrophic, they are sessile.

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Dan Kliebenstein @spicybotrytis.bsky.social · 7d

If you were the predicting sort, how much of a plants metabolism would be in the following four categories?

Primary Anabolism
Primary Catabolism
Specialized Anabolism
Specialized Catabolism

Feel free to define "how much" by gene #, enzyme #, flux fraction, etc.

And your own 1 v 2 defn.

#secmet

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Dan Kliebenstein @spicybotrytis.bsky.social · 7d

At least it didn't autocorrect to sweetbreads.

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Dan Kliebenstein @spicybotrytis.bsky.social · 7d

We did some modelling using Arabidopsis population parameters and the typical enzyme gene will be fixed for stop codons in about 10,000 generations once it has 0 effect on fitness. Unsure how to model whole gene deletion events as we see whole gene deletions more then fixed stops.

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