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Samuel Haysom

@shaysom.bsky.social

10 followers 43 following 3 posts

Structural biologist at Nxera Pharma | CryoEM | drug discovery | GPCRs | in silico protein design

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Reposted by Samuel Haysom

RadfordLab @radford-lab.bsky.social · 24d

See our latest paper on BAM action. Great work to the team and super collaborations with @naranson.bsky.social @antoncalabrese.bsky.social and @Jean Francois collet. Have a read rdcu.be/eJjDp

Molecular insights into how the motions of the β-barrel and POTRA domains of BamA are coupled for efficient function

Nature Communications - BamA is the catalytic core of the BAM complex, which inserts proteins into bacterial outer membranes. Here, the authors show that hinge flexibility between BamA’s...

rdcu.be

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Reposted by Samuel Haysom

Augustinas Silale @augustsilale.bsky.social · 26d

Berks and @smlea.bsky.social labs also found yet a different BAM architecture in Flavobacterium johnsoniae, another member of Bacteroidota.

doi.org/10.1038/s415...

A new paradigm for outer membrane protein biogenesis in the Bacteroidota - Nature

Structural and biochemical studies of the β-barrel-assembly machinery from Flavobacterium johnsoniae reveal a subunit composition and assembly that are distinct from those of the canonical Escherichia coli complex.

doi.org

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Samuel Haysom @shaysom.bsky.social · Sep 11

Any chance this will be recorded?

Samuel Haysom @shaysom.bsky.social · May 17

The contrast thing is interesting, in our samples (GPCRs) our in house data (Glacios F4i) often has worse orientations than data collected on the same grids on Krios’s with energy filters. Thinking the higher contrast/signal from the EF may helping with recovery of the rarer views

Samuel Haysom @shaysom.bsky.social · May 17

Very interesting that they actually got better results with orientation rebalancing when they reject particles randomly rather than by a specific metric (3D alignments etc.). Will definitely have to start testing that in my data

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