Brian English
@sciencethecat.bsky.social
senior scientist at Janelia | single particle tracking & #microscopy 🔬 | PhD on single-molecule enzymatic dynamics | my path: 🇩🇪→🇺🇸→🇸🇪→🇺🇸 | https://scholar.google.com/citations?user=UzGfR3MAAAAJ&hl=en
Reposted by Brian English
Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.
November 21, 2025 at 3:54 AM
Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.
Reposted by Brian English
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
Reposted by Brian English
We moved the AI@MBL course "Deep Learning for Microscopy Image Analysis" to HHMI Janelia (@hhmijanelia.bsky.social).
Join us for two weeks of intense lectures, exercise, and hands-on project work!
Course dates: June 4-18 2026
Application by: January 15 2026
www.janelia.org/you-janelia/...
Join us for two weeks of intense lectures, exercise, and hands-on project work!
Course dates: June 4-18 2026
Application by: January 15 2026
www.janelia.org/you-janelia/...
November 17, 2025 at 7:44 PM
We moved the AI@MBL course "Deep Learning for Microscopy Image Analysis" to HHMI Janelia (@hhmijanelia.bsky.social).
Join us for two weeks of intense lectures, exercise, and hands-on project work!
Course dates: June 4-18 2026
Application by: January 15 2026
www.janelia.org/you-janelia/...
Join us for two weeks of intense lectures, exercise, and hands-on project work!
Course dates: June 4-18 2026
Application by: January 15 2026
www.janelia.org/you-janelia/...
Reposted by Brian English
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Brian English
TODAY (Sunday Nov 9th) starting at 4pm. Please join me in an interactive performance at the Athenaeum in Alexandria VA #interactiveart #performanceart
November 9, 2025 at 3:27 PM
TODAY (Sunday Nov 9th) starting at 4pm. Please join me in an interactive performance at the Athenaeum in Alexandria VA #interactiveart #performanceart
Reposted by Brian English
Exciting draw and late @fcbayernmunich.bsky.social comeback against Union Berlin in der alten Försterei. See you next Saturday morning when we play Freiburg at home #miasanfamily♥️ #washingtondc #MiaSanMia
November 8, 2025 at 6:35 PM
Exciting draw and late @fcbayernmunich.bsky.social comeback against Union Berlin in der alten Försterei. See you next Saturday morning when we play Freiburg at home #miasanfamily♥️ #washingtondc #MiaSanMia
Reposted by Brian English
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....
onlinelibrary.wiley.com
October 25, 2025 at 8:56 AM
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
Reposted by Brian English
Our confirmed count stands at 8.1 million participants worldwide for yesterday’s No Kings protest. Thank you to everyone who helped verify the numbers through our submission website. While some MAGAs attempted to submit false or misleading data (such as reporting “one person” for major cities)
October 19, 2025 at 11:03 PM
Our confirmed count stands at 8.1 million participants worldwide for yesterday’s No Kings protest. Thank you to everyone who helped verify the numbers through our submission website. While some MAGAs attempted to submit false or misleading data (such as reporting “one person” for major cities)
Reposted by Brian English
Very excited to be co-organizing this meeting. Hope to see you there!
Have you submitted your abstract for #EESCollectivity? No? It's about time! This new event will discuss how collective behaviours arise from fundamental principles across biological systems, from cells to animal groups. 🦠🐾
👉 Join us and submit your abstract by 18 Nov: s.embl.org/ees26-01-bl
👉 Join us and submit your abstract by 18 Nov: s.embl.org/ees26-01-bl
October 17, 2025 at 11:38 AM
Very excited to be co-organizing this meeting. Hope to see you there!
Reposted by Brian English
Maud Menten was born in Canada in 1879 and completed her UG and MD education at University of Toronto. As a research assistant with Leonor Michaelis in 1912, they wrote the classic paper describing the “Michaelis-Menten” model of enzyme kinetics.. #WomenInScience
October 16, 2025 at 1:02 AM
Maud Menten was born in Canada in 1879 and completed her UG and MD education at University of Toronto. As a research assistant with Leonor Michaelis in 1912, they wrote the classic paper describing the “Michaelis-Menten” model of enzyme kinetics.. #WomenInScience
Just heard him speak at Janelia just now. Wonderful talk!
Jay Groves starts off the last day of #BiomembraneDays2025 with a deep dive into signaling at the membrane.
October 8, 2025 at 4:41 PM
Just heard him speak at Janelia just now. Wonderful talk!
Reposted by Brian English
(Part 1) Hear from @hhmi_news Investigator David Stern about his research program and what he's uncovering in the lab.
Stern will join the Institute in February 2026, bringing his lab and HHMI appointment to Kansas City. @hhmi.org
Read more: bit.ly/4np96Fb
Stern will join the Institute in February 2026, bringing his lab and HHMI appointment to Kansas City. @hhmi.org
Read more: bit.ly/4np96Fb
September 30, 2025 at 5:10 PM
(Part 1) Hear from @hhmi_news Investigator David Stern about his research program and what he's uncovering in the lab.
Stern will join the Institute in February 2026, bringing his lab and HHMI appointment to Kansas City. @hhmi.org
Read more: bit.ly/4np96Fb
Stern will join the Institute in February 2026, bringing his lab and HHMI appointment to Kansas City. @hhmi.org
Read more: bit.ly/4np96Fb
Reposted by Brian English
"Her work made it impossible to separate what we learn from our obligation to protect it. Generations of researchers have tried to follow that example." - Jennifer Doudna (2/2)
October 2, 2025 at 1:48 PM
"Her work made it impossible to separate what we learn from our obligation to protect it. Generations of researchers have tried to follow that example." - Jennifer Doudna (2/2)
Reposted by Brian English
The culmination of many years work. One of the most advanced light-sheet microscopes in the world!
-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!
For details see previous posts and here: github.com/amsikking/HT...
-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!
For details see previous posts and here: github.com/amsikking/HT...
September 28, 2025 at 6:33 PM
The culmination of many years work. One of the most advanced light-sheet microscopes in the world!
-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!
For details see previous posts and here: github.com/amsikking/HT...
-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!
For details see previous posts and here: github.com/amsikking/HT...
Reposted by Brian English
Congratulations Jie! @jiexiaolab.bsky.social
Jie Xiao to Receive 2026 Carolyn Cohen Innovation Award
www.biophysics.org/news-room/ji...
www.biophysics.org/news-room/ji...
September 24, 2025 at 8:22 PM
Congratulations Jie! @jiexiaolab.bsky.social
Reposted by Brian English
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Brian English
Nobel laureate Jennifer Doudna tells Jeffrey Goldberg that vaccinations have been so successful that "there's been a collective forgetting that measles, and mumps, and rubella—these used to be diseases that would kill people in fairly large numbers." #TAF25 bit.ly/46a5DUE
September 18, 2025 at 4:34 PM
Nobel laureate Jennifer Doudna tells Jeffrey Goldberg that vaccinations have been so successful that "there's been a collective forgetting that measles, and mumps, and rubella—these used to be diseases that would kill people in fairly large numbers." #TAF25 bit.ly/46a5DUE
Reposted by Brian English
Today’s work, aside from meetings.
September 12, 2025 at 11:34 PM
Today’s work, aside from meetings.
Reposted by Brian English
@embl.org Heidelberg is looking for a new Head of Scientific Instrumentation! This is an exciting opportunity at the interface between scientific research and technology development, overseeing our mechanical and electronic workshops!
embl.wd103.myworkdayjobs.com/en-US/EMBL/d...
embl.wd103.myworkdayjobs.com/en-US/EMBL/d...
Head of Scientific Instrumentation Workshops
EMBL is a world leader in scientific research and at the forefront in the development of cutting-edge technologies. It operates across six sites in Heidelberg (headquarters), Barcelona, Cambridge, Gre...
embl.wd103.myworkdayjobs.com
September 12, 2025 at 6:48 PM
@embl.org Heidelberg is looking for a new Head of Scientific Instrumentation! This is an exciting opportunity at the interface between scientific research and technology development, overseeing our mechanical and electronic workshops!
embl.wd103.myworkdayjobs.com/en-US/EMBL/d...
embl.wd103.myworkdayjobs.com/en-US/EMBL/d...
Reposted by Brian English
Beautiful work led by @oliveringe.bsky.social. It was great fun to be involved with this project.
See Ollie’s paper and thread 👇
See Ollie’s paper and thread 👇
1/7 Really happy to see my PhD work published in ✨Developmental Cell✨ today! We find human endoderm is specified by two developmental trajectories 🔀, and the choice between alternate routes is dictated by the combinatorial BMP4/Activin signalling. www.cell.com/developmenta...
Combinatorial BMP4 and activin direct the choice between alternate routes to endoderm in a stem cell model of human gastrulation
Inge et al. show that human endoderm originates from two converging developmental
routes with distinct dynamics and efficiencies yet similar developmental potential.
Combinatorial activin and BMP4 sig...
www.cell.com
September 9, 2025 at 8:17 PM
Beautiful work led by @oliveringe.bsky.social. It was great fun to be involved with this project.
See Ollie’s paper and thread 👇
See Ollie’s paper and thread 👇
Reposted by Brian English
Thanks Misha for the shout-out!
It has been a truly mind-opening journey. WHOLISTIC is one step towards whole-organism neuroscience and a true physiological observatory - with extensions on the way! I am continually amazed by what the data reveals - so much remains to be discovered and mined.
It has been a truly mind-opening journey. WHOLISTIC is one step towards whole-organism neuroscience and a true physiological observatory - with extensions on the way! I am continually amazed by what the data reveals - so much remains to be discovered and mined.
Preprint -
Excited to present WHOLISTIC, which extends the concept of whole-brain functional imaging to the entire body. Pioneering work by incredibly talented Virginia Ruetten @vmsruetten.bsky.social, this platform reveals whole-organism cellular dynamics in vivo.
www.biorxiv.org/content/10.1...
Excited to present WHOLISTIC, which extends the concept of whole-brain functional imaging to the entire body. Pioneering work by incredibly talented Virginia Ruetten @vmsruetten.bsky.social, this platform reveals whole-organism cellular dynamics in vivo.
www.biorxiv.org/content/10.1...
September 8, 2025 at 4:30 PM
Thanks Misha for the shout-out!
It has been a truly mind-opening journey. WHOLISTIC is one step towards whole-organism neuroscience and a true physiological observatory - with extensions on the way! I am continually amazed by what the data reveals - so much remains to be discovered and mined.
It has been a truly mind-opening journey. WHOLISTIC is one step towards whole-organism neuroscience and a true physiological observatory - with extensions on the way! I am continually amazed by what the data reveals - so much remains to be discovered and mined.
Reposted by Brian English
We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)
September 4, 2025 at 8:22 AM
We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)
Reposted by Brian English
Results so stunningly clear they inspired this classic xkcd (xkcd.com/2400/):
September 1, 2025 at 7:32 PM
Results so stunningly clear they inspired this classic xkcd (xkcd.com/2400/):