Jon Daniels
@jsdaniel02.bsky.social
Scientific instrument developer, focused on #lightsheet and other #microscopy 🔬 at ASI
I'm working on an objective lens for expansion microscopy. I have a candidate design to share. Looking for feedback before we finalize things.
NA 1.0 water-dipping
5.5mm WD
1.1mm FOV, plan-corrected
8mm EFL ("25x")
corrected in visible with exception noted below
minimum mechanical profile
NA 1.0 water-dipping
5.5mm WD
1.1mm FOV, plan-corrected
8mm EFL ("25x")
corrected in visible with exception noted below
minimum mechanical profile
January 22, 2026 at 11:07 PM
I'm working on an objective lens for expansion microscopy. I have a candidate design to share. Looking for feedback before we finalize things.
NA 1.0 water-dipping
5.5mm WD
1.1mm FOV, plan-corrected
8mm EFL ("25x")
corrected in visible with exception noted below
minimum mechanical profile
NA 1.0 water-dipping
5.5mm WD
1.1mm FOV, plan-corrected
8mm EFL ("25x")
corrected in visible with exception noted below
minimum mechanical profile
I highly recommend this course as an introduction to microscopy.
PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...
Quantitative Imaging: From Acquisition to Analysis
Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.
meetings.cshl.edu
January 22, 2026 at 1:25 AM
I highly recommend this course as an introduction to microscopy.
Reposted by Jon Daniels
Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...
January 19, 2026 at 7:04 PM
Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...
I'm working on a new water-dipping objective targeting expansion imaging. Targeting the following specs:
5mm WD
NA 1.0
1.2mm FOV, plan-corrected
8mm EFL ("25x") but this is flexible
well-corrected in visible
minimum mechanical profile
Optics question: is there any reason to make it telecentric?
5mm WD
NA 1.0
1.2mm FOV, plan-corrected
8mm EFL ("25x") but this is flexible
well-corrected in visible
minimum mechanical profile
Optics question: is there any reason to make it telecentric?
December 18, 2025 at 7:54 PM
I'm working on a new water-dipping objective targeting expansion imaging. Targeting the following specs:
5mm WD
NA 1.0
1.2mm FOV, plan-corrected
8mm EFL ("25x") but this is flexible
well-corrected in visible
minimum mechanical profile
Optics question: is there any reason to make it telecentric?
5mm WD
NA 1.0
1.2mm FOV, plan-corrected
8mm EFL ("25x") but this is flexible
well-corrected in visible
minimum mechanical profile
Optics question: is there any reason to make it telecentric?
The ASI machine shop made a nice replica model of the AMS-AGY v3 objective for us to have in the #CellBio2025 booth. The first of the real ones will be ready by the end of the year.
December 9, 2025 at 2:33 PM
The ASI machine shop made a nice replica model of the AMS-AGY v3 objective for us to have in the #CellBio2025 booth. The first of the real ones will be ready by the end of the year.
If you are at #CellBio2025 stop by and say hello at booth 1101
December 7, 2025 at 5:51 PM
If you are at #CellBio2025 stop by and say hello at booth 1101
Great to see the ASI single-objective light sheet used for long-term imaging of tumor cells to study the transition between single-cell and multi-cellular spheroids: www.biorxiv.org/content/10.1...
Cells dynamically adapt their nuclear volumes and proliferation rates during single to multicellular transitions
Tumour development and progression are associated with biophysical alterations that manifest across multiple spatial scales, from the subcellular to multicellular tissue scale. While cells can dynamic...
www.biorxiv.org
October 28, 2025 at 5:11 PM
Great to see the ASI single-objective light sheet used for long-term imaging of tumor cells to study the transition between single-cell and multi-cellular spheroids: www.biorxiv.org/content/10.1...
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
July 9, 2025 at 8:00 PM
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
I'm excited to be able to be in Zurich for the mesoSPIM symposium. This is a really fantastic project.
May 15, 2025 at 6:24 PM
I'm excited to be able to be in Zurich for the mesoSPIM symposium. This is a really fantastic project.
Most high-NA air objective lenses are coverslip-corrected but sometimes you want to image in air, e.g. high-NA SOLS. We have glued coverslips onto objectives for this. To make sure it gets glued properly we take image stack of diffraction-limited beads supported on a coverslip. 🧵
March 14, 2025 at 8:39 PM
Most high-NA air objective lenses are coverslip-corrected but sometimes you want to image in air, e.g. high-NA SOLS. We have glued coverslips onto objectives for this. To make sure it gets glued properly we take image stack of diffraction-limited beads supported on a coverslip. 🧵
Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer.bsky.social and Bin Yang. vimeo.com/801341925
Zebrafish embryo development acquired on the DaXi light-sheet microscope
Volumetric imaging of zebrafish embryo development acquired on the DaXi light-sheet microscope. Imaging volume is 1200 μm * 800 μm * 300 μm acquired every…
vimeo.com
December 6, 2024 at 4:47 PM
Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer.bsky.social and Bin Yang. vimeo.com/801341925