joe dobbs
@joedobbs.bsky.social
91 followers 110 following 11 posts
structural cell biologist @EMBL 🇨🇦
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joedobbs.bsky.social
Thanks! According to their stats page (www.ebi.ac.uk/emdb/statist...) I should be at around 37% right now, but we'll have to see how the rest of the year goes! 📈
Electron Microscopy Data Bank
www.ebi.ac.uk
joedobbs.bsky.social
Thanks! I didn't see h44 repositioning despite directly looking for it. Could be the P-tRNA, but I also suspect IF3 might be involved in clamping it down in some way (>90% of 30S had IF3).
joedobbs.bsky.social
Thanks! Although my wrists might disagree :)
joedobbs.bsky.social
Sadly not :( I got most of the model IDs sequential, but the maps were a different story
joedobbs.bsky.social
Thanks!! Very true, and things are getting more and more doable- I think it'll be quite a different place in a few years.
joedobbs.bsky.social
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️

www.biorxiv.org/content/10.1...
#teamtomo
Figure 1 from the preprint
joedobbs.bsky.social
New paper from my colleague Soheil in the Mahamid and @jonasries.bsky.social groups looking at factors influencing laser devitrification on frozen #cryoem samples. With a far-red fluorophore, all-gold support grids will be especially good for correlative cryo-SMLM and EM!

doi.org/10.1016/j.js...
Reposted by joe dobbs
rasmusjensen.bsky.social
Are you into cryo-ET, in-cell discovery, protein translocation and folding or just cool integration of structural biology, proteomics and bioinformatics? Then see our new work:

www.biorxiv.org/content/10.1...

Thank you to all coauthors for your amazing work!
www.biorxiv.org