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A recent cool preprint by John Whitney's lab on a new family of antibacterial proteins secreted by Gram-positive bacteria that enter and kill a broad spectrum of bacteria. Cell entry is receptor-independent and relies on cleavage by a co-secreted protease and the PMF.
www.biorxiv.org/content/10.1...

This is insanely cool!!

The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.

Diversity-generating retroelements are a fascinating class of targeted gene editing system. How DGRs function within cells remains incompletely understood. In this preprint (www.biorxiv.org/content/10.1...
), we report identification of factors influencing DGR activity in E. coli.
#MicroSky

🚨👉 Please check our recent work on bacterial cell division. In situ Cryo-ET reveals the cellular function of the penicillin binding protein 1b supported by AFM, live-cell imaging, in silico AlphaFold proteome screen and TIRFM. Hope you enjoy the read! #teamtomo #cryo-ET ❄️🔬🐎 big thanks to the team!

🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4

How do cells sort which RNAs to keep or destroy? New preprint from THJ, Brenneke and Plaschka labs shows that export and decay machineries (TREX2/PAXT) both recognise UAP56-bound RNAs. Whether they’re exported or degraded depends on where in the nucleus this happens.
www.biorxiv.org/content/10.1...

PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.

But how does piRNA-guided target interaction translate into silencing?

PhD student Júlia Portell Montserrat has an intriguing answer

www.cell.com/molecular-ce...

Congrats to Apurva, Brian and Irem and a big thank you to our collaborators:
• Brooke of the Kim lab (UIUC) for microscopy
• Molly of the Helaine lab (Harvard Med) for tRNA-seq
• Eric of the Corrêa lab (NEB) for cleavage site mapping by mass spec

Key highlights:
• A phage-inspired genetic screen to identify the targets of tRNA nucleases.
• OLD severs the anticodon stem with a one-base staggered cut.
• tRNAs destroyed by OLD are irreparable by T4 RNA ligase. • Both cuts occur after the first cytosine within a paired CNG pseudo-palindrome.

First paper from my lab is now published! academic.oup.com/nar/article-... P2-lambda interference was first reported in the 1950's, but how a P2 lysogen restricts lambda replication has remained murky. We uncovered tRNA cleavage by P2-OLD as a key mechanism behind this interference.

We're looking for a technician at Institut Pasteur for experimental work on satellite-phage-bacteria interactions, working directly with @jmouradesousa.bsky.social and myself in the lab of @epcrocha.bsky.social ! ANR funded.

Link to the job emploi.pasteur.fr/offre-de-emp...
Thx for sharing!

Congrats!!

Bacterial cell wall & periplasm paradigm shift - important read #MicroSky

www.nature.com/articles/s41...