Eloïse Bertiaux
@ebertiaux.bsky.social
Reposted by Eloïse Bertiaux
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system
Cryo-EM structure reveals how a ubiquitin-like protein orchestrates assembly of a bacterial weapon system.
www.science.org
November 21, 2025 at 7:05 PM
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
Honored to receive the FEBS Booster Funds 2025.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
November 18, 2025 at 12:54 PM
Honored to receive the FEBS Booster Funds 2025.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
Reposted by Eloïse Bertiaux
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
October 14, 2025 at 1:50 PM
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
Reposted by Eloïse Bertiaux
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
October 13, 2025 at 12:55 PM
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
Reposted by Eloïse Bertiaux
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 7:03 AM
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Reposted by Eloïse Bertiaux
Be ready for an exciting meeting!
Save the date!
March 2027 on beautiful lake Maggiore right outside #Milan, are you ready for #Cilia2027? 🥳
Stay tuned for more info!
March 2027 on beautiful lake Maggiore right outside #Milan, are you ready for #Cilia2027? 🥳
Stay tuned for more info!
July 29, 2025 at 10:12 AM
Be ready for an exciting meeting!
Reposted by Eloïse Bertiaux
🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
July 21, 2025 at 9:07 AM
🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Reposted by Eloïse Bertiaux
💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
June 10, 2025 at 9:15 AM
💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
Reposted by Eloïse Bertiaux
On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions!
Many thanks for everything!
journals.biologists.com/jcs/article-...
Many thanks for everything!
journals.biologists.com/jcs/article-...
July 7, 2025 at 12:36 PM
On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions!
Many thanks for everything!
journals.biologists.com/jcs/article-...
Many thanks for everything!
journals.biologists.com/jcs/article-...
Reposted by Eloïse Bertiaux
#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller.
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
June 27, 2025 at 9:31 AM
#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller.
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Reposted by Eloïse Bertiaux
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
June 19, 2025 at 8:58 AM
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Reposted by Eloïse Bertiaux
Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!
May 27, 2025 at 8:01 PM
Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!
Reposted by Eloïse Bertiaux
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc
May 19, 2025 at 9:09 AM
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc
Reposted by Eloïse Bertiaux
Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...
March 19, 2025 at 8:03 AM
Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...
Reposted by Eloïse Bertiaux
Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms | Science www.science.org/doi/10.1126/...
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms
The flagellum of Trypanosoma brucei drives the parasite’s characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this ...
www.science.org
March 13, 2025 at 9:00 PM
Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
Reposted by Eloïse Bertiaux
Assistant prof / MCF post available in ProParaCyto.
March 7, 2025 at 8:17 AM
Assistant prof / MCF post available in ProParaCyto.
Reposted by Eloïse Bertiaux
March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234).
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
March 3, 2025 at 11:00 AM
March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234).
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Reposted by Eloïse Bertiaux
A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together!
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
mSphere of Influence: The ever-expanding universe of parasite cell biology | mSphere
Single-celled apicomplexan parasites have to grow, replicate, and divide all of the
organelles of a eukaryotic cell all while being a tiny fraction of the size of most
other eukaryotes. For example, a...
journals.asm.org
March 4, 2025 at 10:01 PM
A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together!
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Reposted by Eloïse Bertiaux
Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
March 4, 2025 at 8:07 AM
Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
Reposted by Eloïse Bertiaux
A video clip to see #trypanosomes in #3D #FIB-SEM
Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr
www.pasteur.fr/fr/journal-r...
Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr
www.pasteur.fr/fr/journal-r...
Le coupable de la Maladie du Sommeil
Plongée inédite au cœur du trypanosome, le parasite responsable de la maladie du sommeil !Transmise par la piqûre de la mouche tsé-tsé, cette maladie touche plus de 30 pays d’Afrique subsaharienne. Ap...
www.pasteur.fr
February 17, 2025 at 8:45 AM
A video clip to see #trypanosomes in #3D #FIB-SEM
Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr
www.pasteur.fr/fr/journal-r...
Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr
www.pasteur.fr/fr/journal-r...
Reposted by Eloïse Bertiaux
We propose a model where the new flagellum grows in a linear manner while the old one is locked but shrinks slightly, hence requiring unlocking and compensation after cell division, leading to such multicoloured flagella following induction/deinduction/reinduction of tagged tubulin 😉
February 11, 2025 at 8:54 AM
We propose a model where the new flagellum grows in a linear manner while the old one is locked but shrinks slightly, hence requiring unlocking and compensation after cell division, leading to such multicoloured flagella following induction/deinduction/reinduction of tagged tubulin 😉
Reposted by Eloïse Bertiaux
Solving 25 years of frustration, Daniel Abbühl managed to tag trypanosome tubulin and to express it in an inducible manner, hence revealing flagellum assembly! A tour-de-force!
Preprint here:
www.biorxiv.org/content/10.1...
Preprint here:
www.biorxiv.org/content/10.1...
February 11, 2025 at 8:54 AM
Solving 25 years of frustration, Daniel Abbühl managed to tag trypanosome tubulin and to express it in an inducible manner, hence revealing flagellum assembly! A tour-de-force!
Preprint here:
www.biorxiv.org/content/10.1...
Preprint here:
www.biorxiv.org/content/10.1...
Reposted by Eloïse Bertiaux
Parasite of the month in Trends in Parasitology: #Trypanosoma equiperdum. By Perrine Hervé, Frédéric Bringaud and Loïc Rivière (ParaFrap Team) @cp-trendsparasitol.bsky.social
Parasite of the Month: #Trypanosoma #equiperdum, the #etiological agent of #dourine, a disease specific to #equids ( #horses #mules #donkeys ) transmitted through #coitus, authored by P. Hervé, F. Bringaud & L. Rivière @univbordeaux.bsky.social @cnrs.bsky.social
authors.elsevier.com/a/1kVtS5Eb1x...
authors.elsevier.com/a/1kVtS5Eb1x...
February 10, 2025 at 10:34 AM
Parasite of the month in Trends in Parasitology: #Trypanosoma equiperdum. By Perrine Hervé, Frédéric Bringaud and Loïc Rivière (ParaFrap Team) @cp-trendsparasitol.bsky.social
Reposted by Eloïse Bertiaux
Very interesting work here: centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends rupress.org/jcb/article/...
Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends | Journal of Cell Biology | Rockefeller University Press
Iyer et al. combine in vitro reconstitution assays with cryo-electron tomography, biophysical, and cell biological approaches to show that two centriolar p
rupress.org
January 24, 2025 at 8:29 AM
Very interesting work here: centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends rupress.org/jcb/article/...
Reposted by Eloïse Bertiaux
Our paper on U-ExM for mosquito tissues (MoTissU-ExM) in BMC methods!
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito - BMC Methods
Background Study of malaria parasite cell biology is challenged by their small size, which can make visualisation of individual organelles difficult or impossible using conventional light microscopy. ...
bmcmethods.biomedcentral.com
January 19, 2025 at 11:16 PM
Our paper on U-ExM for mosquito tissues (MoTissU-ExM) in BMC methods!
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....