Author | Lightnews

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · 17d

This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.

1 5

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Sep 12

This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron

2 4

Louise Johnson @drlouisejohnson.bsky.social · Jun 21

Happy #Caturday! Princess Fifi Trixibelle Murder Mittens (first of her name) is enjoying the weekend #cats #adoptdontshop #pond

2 9

Louise Johnson @drlouisejohnson.bsky.social · May 4

I am thinking the left image is AI… it is too perfect. Which is the correct though?

1

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · May 2

Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron

1 3 5

Louise Johnson @drlouisejohnson.bsky.social · Apr 27

I wish “smell-o-vision” was a thing, as these #lilyofthevalley smell incredible. #Spring #April #gardenpond #nativeplant #uk

3

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Apr 25

Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope.

#immunology #animalfree

2 6

Louise Johnson @drlouisejohnson.bsky.social · Apr 21

Looking through the leper’s squint into a church that is approaching its 1000th birthday #uk #history #england

Louise Johnson @drlouisejohnson.bsky.social · Apr 12

Happy #Caturday! Not sure how long my Easter decorations will stay in place though

6

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Apr 7

#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron

1 5

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Apr 4

This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron

1 7

Louise Johnson @drlouisejohnson.bsky.social · Mar 28

I disturbed another occupant of the Oxford Science Park but they were illegally fishing - goldfish saved!

1

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 28

Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope

1 2 5

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 21

This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social

1 4

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 14

#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social

1 6

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 13

Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.

doi.org/10.1208/s122...
#BLI #immunology #animalfree

1 2

Reposted by Louise Johnson

British Society for Immunology @britsocimm.bsky.social · Mar 11

🚨 Last chance to register for BSI Oxford and @immunologyoxford.bsky.social's Oxford Immunology Symposium! #OIS25

📅 Date: 24 March 2025
📍 Location: Oxford Mathematical Institute
🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights

Register NOW: t.co/tlXp9dddqo

10 9

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 7

It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs
@afruk.bsky.social

1 11

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Mar 5

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social

1 1 1

Louise Johnson @drlouisejohnson.bsky.social · Feb 28

I love being part of this project and product line. Also, continually impressed at the sharpness of the images we achieve on our (epifluorescence) Echo Revolve microscope through the digital haze reduction software

ProImmune @proimmune.bsky.social · Feb 28

Fab fine filaments of the cytoskeleton for #FluorescenceFriday lit up by our #animalfree Ankyron™ to β-actin (red), with nuclei counterstained with DAPI (blue) on our Echo Revolve #microscope @discoverecho.bsky.social An alternative counterstain to phalloidin! #immunology #3Rs @afruk.bsky.social

1

Reposted by Louise Johnson

ProImmune @proimmune.bsky.social · Feb 21

Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope
#bioscience #immunology #3Rs
@AFR_UK

2 7

Louise Johnson @drlouisejohnson.bsky.social · Feb 19

And how the skylight looks from the inside

Louise Johnson @drlouisejohnson.bsky.social · Feb 19

For #WindowsWednesday a view of Stourhead #nationaltrust from a room not usually open to the public. The guides were amazing and really brought it to life

1 1

Louise Johnson @drlouisejohnson.bsky.social · Feb 18

Ooo I do like the thought about the hair - let’s hope they lived happily ever after and exchanged hair products for all their days ❤️

1 1

Louise Johnson @drlouisejohnson.bsky.social · Feb 18

Am I just being a cynic but isn’t she looking into the jewels on his crown rather than into his eyes?!

1 2