Healthcare-associated infections pose a significant health risk for inpatients. The Centers for Disease Control and Prevention estimates that there are over 1.7 million healthcare-associated infection...

Endothelial cells (ECs) play a critical role in managing vascular homeostasis and neovascularization. EC functions vary significantly depending on their anatomic locations, especially for ECs forming macrovascular versus microvascular vessels. ECs possess heterogeneous signaling pathways, energy metabolism, and cellular behaviors that enable them to handle both physiological and hyperglycemic conditions. These variations can impact the efficacy of pharmacotherapy and influence the likelihood of unexpected side effects. In this study, we compared human aortic ECs (HAECs) and human dermal microvascular ECs (HDMVECs) to observe the functional and proteomic differences potentially contributing to EC heterogeneity. Compared to HAECs, HDMVECs exhibited faster proliferation, but lower migration and permeability. Under high glucose, migration was worsened for both cell types, whereas proliferation was unaffected, and permeability increased for HDMVECs. Using proteomic analysis, we identified 126 proteins whose abundance was significantly different between HAECs and HDMVECs. DAVID analysis revealed their biological processes, cellular compartments, molecular functions, and pathways. under high glucose, WARS1 increased while SOD2 decreased. Reversing WARS1 or SOD2 expression levels improved HDMVEC migration and permeability functions. The combined treatment of WARS1 knockdown and SOD2 overexpression fully restored EC migration and reduced permeability to levels comparable to those of their counterparts under normal glucose conditions. Furthermore, in the cutaneous wound in type 2 diabetic mice, the combination therapy of Wars1 knockdown and SOD2 overexpression accelerated the wound closure and augmented wound angiogenesis. Our studies provide novel molecular insights into EC heterogeneity and identified WARS1 and SOD2 as potential targets for dermal angiogenesis during tissue repair.

Background: The use of artificial intelligence (AI)–based large language model chatbots such as ChatGPT has become increasingly popular in many disciplines. However, concerns exist regarding ethics, l...

AbstractBackground. New Delhi metallo-beta-lactamases (NDM) confer extensive antibiotic resistance, complicating treatment and leading to poor outcomes. In

Background: Diabetic ketoacidosis (DKA) is a severe complication of diabetes mellitus requiring insulin. However, there is no consensus on insulin infusion titr...

Patients with persistent methicillin-resistant Staphylococcus aureus (MRSA) infections, which are associated with high mortality rates, often require prolonged hospitalizations, intensive care service...

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AbstractPurpose. Care fragmentation occurs when patients use multiple providers, health systems, and pharmacies, leading to polypharmacy, potentially inapp

As environmental exposomes are changing with time, so are liver diseases around the globe. Due to an increasing prevalence of overnutrition and sedentary lifestyle in the global population, metabolic ...

Abstract. Antimicrobial stewardship programs (ASPs) are underrepresented in outpatient settings, where antibiotic use and overprescribing are common. Upper

We evaluated implementation of a shortened post-operative vancomycin prophylaxis duration protocol on acute kidney injury (AKI) in lung transplant recipients. The intervention, modifying vancomycin (...

Background: In 2024, national guidance was issued for doxycycline post-exposure prophylaxis (doxy-PEP) to prevent bacterial sexually transmitted infections (STI...

Characterization of the molecular mechanisms of VISTA and elucidation of therapeutic vulnerabilities in a class of triple-negative breast cancer that strongly expresses VISTA in tumor cells could info...

Contemporary pharmaceutical design often incorporates functional groups to improve the pharmacokinetic/pharmacodynamic profile of a desired active chemical entity. These compounds are known as prodrug...

A little nudge goes a long way: assessing the impact of a microbiology nudge comment on narrow-spectrum antibiotic use in uncomplicated Streptococcus pneumoniae bloodstream infections - Volume 5 Issue...

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We first met the summer before starting pharmacy school at the Eugene Applebaum College of Pharmacy and Health Sciences at Wayne State University. Excited