Lightnews — Scholar-powered news

Spirochrome @spirochrome.com · 8d

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.

Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

Juanma García-Arcos @juanmagararc.bsky.social · 10d

🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM

Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...

www.biorxiv.org

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Reposted by Spirochrome

Spirochrome @spirochrome.com · 13d

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging

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Spirochrome @spirochrome.com · 13d

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging

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Reposted by Spirochrome

Daniel J. Cohen (Princeton) @djcohen.bsky.social · 28d

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

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Reposted by Spirochrome

Alexandre Dumoulin @axonsalex.bsky.social · 16d

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Spirochrome @spirochrome.com · 19d

Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!

His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.

www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday

In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition

Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)

www.nikonsmallworld.com

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Spirochrome @spirochrome.com · 19d

Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!

His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.

www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday

In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition

Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)

www.nikonsmallworld.com

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Reposted by Spirochrome

Spirochrome @spirochrome.com · 26d

HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.

Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...

Image credits: Olivier Mercey, UNIGE

U-ExM expanded HeLa cell stained with HAK-actin (orange) and DNA (blue).

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Spirochrome @spirochrome.com · 26d

HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.

Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...

Image credits: Olivier Mercey, UNIGE

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Spirochrome @spirochrome.com · 27d

Amazing achievement!

Congratulations

Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍

Daniel J. Cohen (Princeton) @djcohen.bsky.social · 28d

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

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Spirochrome @spirochrome.com · 29d

Congratulations! We're very much looking forward to seeing what's coming up with the future T-cell imaging!

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Akaash Kumar @akaashkumar.bsky.social · 29d

🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...

Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics

A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...

www.nature.com

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Reposted by Spirochrome

Nikon Microscope Solutions @healthcare.nikon.com · Sep 10

📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure).

🔗 View the preprint article: bit.ly/47xC8NJ

#Microscope #Imaging #Fluorescence

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Reposted by Spirochrome

Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · Sep 10

For all of you #Actin folks ... It's out !
Happy to help at anypoint

Spirochrome @spirochrome.com · Sep 10

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

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Reposted by Spirochrome

Spirochrome @spirochrome.com · Sep 10

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

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Reposted by Spirochrome

Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Sep 10

🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.

Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution

Spirochrome @spirochrome.com · Sep 10

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

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Spirochrome @spirochrome.com · Sep 10

HAK-actin™ was developed in the labs of @centriolelab.bsky.social and @dudinlab.bsky.social.

Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025...

We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie

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Spirochrome @spirochrome.com · Sep 10

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

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Spirochrome @spirochrome.com · Sep 2

A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you ‪@microtubule.bsky.social‬ for sharing it!

Torsten Wittmann @microtubule.bsky.social · Aug 28

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

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Reposted by Spirochrome

Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · Aug 28

A new #UExM probe for #Actin!

Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social !

Congrats to all !

If interested in #UExM, or #HAK-Actin, feel free to reach out !

Soon available at @spirochrome.com

#Expansion #Microscopy #ProtistsOnSky

Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Aug 28

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

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Spirochrome @spirochrome.com · Aug 28

Getting a bright & reliable actin staining in expansion microscopy was a tough nut until today!

The team gathered around @centriolelab.bsky.social found a hak:

HAK-actin, a new probe for U-ExM based protocols revealed in a new BioRxiv manuscript.

HAK-actin will soon be available at Spirochrome!

Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Aug 28

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

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Reposted by Spirochrome

Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Aug 28

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

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Spirochrome @spirochrome.com · Aug 20

SPY650-FastAct_X (red) & SPY555-tubulin (blue) teaming up in this astrocyte to highlight mitochondria (white) and cytoskeleton interactions.

Thank you @aaandmoore.bsky.social‬ for sharing this amazing movie.

Andy Moore @aaandmoore.bsky.social · Aug 18

This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.

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Reposted by Spirochrome

Sara Wickstrom @sarawickstrom.bsky.social · Aug 7

Very happy to see our preprint on how the mechanosensitive ion channel Piezo1 controls intercellular junction maturation by balancing cortex and membrane tension now published in @jcellsci.bsky.social Congratulations to Ahsan & team! journals.biologists.com/jcs/article/...

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Spirochrome @spirochrome.com · Aug 7

Fascinating #mechanobiology article, congratulations! We're delighted that SPY650-FastAct and Flipper-TR worked very well in your experiments.

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Spirochrome @spirochrome.com · Aug 5

Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.

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