Author | Lightnews

Reposted by Mirian Krystel De Siqueira

Nature Metabolism @natmetabolism.nature.com · Jul 5

ICYMI:

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling

Nature Metabolism, Published online: 27 June 2025; doi:10.1038/s42255-025-01320-yDietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

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Reposted by Mirian Krystel De Siqueira

Marcus Tol @marcustol.bsky.social · Jul 2

Excited to share Chapter #1 of our LPCAT3 journey in adipose biology and membrane dynamics—born of a rewarding, years-long, espresso-fueled partnership with Prof. Yuta Shimanaka @shimachu64.bsky.social. www.nature.com/articles/s42...

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism

Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

www.nature.com

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Jun 27

Congratulations, Marc and Yuta!
www.nature.com/articles/s42...

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism

Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

www.nature.com

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · May 19

Check out the preprint of these two amazing scientists, fat-biologists, and superstar former postdocs Yuta and Marc (BlueSky-less users)!

Super excited to see this story out.

www.biorxiv.org/content/10.1...

A cold-inducible phospholipid—protein interaction in brown fat mitochondria optimizes thermogenic capacity

Cold stress elicits dynamic remodeling of the mitochondrial lipidome in brown adipose tissue (BAT), marked by an increase in arachidonoyl–phosphatidylethanolamine (AA–PE). However, the function of mem...

www.biorxiv.org

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Reposted by Mirian Krystel De Siqueira

STAR Protocols @cp-starprotocols.bsky.social · May 1

Protocol to perform polysome profiling in primary differentiating murine adipocytes #protocol #starprotocols #cellpress

Protocol to perform polysome profiling in primary differentiating murine adipocytes

Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. We describe steps for lysate preparation, ultracentrifugation through sucrose gradients, mRNA isolation, RNA sequencing, and motif enrichment. This protocol enables the analysis of actively translating mRNAs and translational gene regulation by isolating polysome-bound mRNA, revealing insights into protein synthesis during adipogenesis or stimuli responses. Applications incl...

dlvr.it

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · May 5

New protocol drop! 🧪 Check out our polysome profiling in primary differentiating murine adipocytes 🐭🧫 — a step-by-step guide to studying translational control during adipogenesis. 👉 star-protocols.cell.com/protocols/4182
@laurahulea.bsky.social @claudiovillanueva.bsky.social

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Reposted by Mirian Krystel De Siqueira

James Olzmann @olzmannlab.bsky.social · Nov 27

Grateful to @berkeleyMCB for highlighting our lab and our recent discoveries! It was fun to discuss some of the surprises during our research on lipid homeostasis, lipid droplets, & ferroptosis—driven by exceptional UC Berkeley students & postdocs. mcb.berkeley.edu/news-and-eve...

Fall 2024 | Research Spotlight: James Olzmann

Faculty Research Spotlight James Olzmann: Big Questions and Aha! Moments By Kirsten Mickelwait James Olzmann James Olzmann’s lab has certainly had its share of “aha!” moments. These come not just from...

mcb.berkeley.edu

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 26

5/5 In a similar way, Rosi drives the blossom of preadipocytes even in unhealthy environments (obesity)! Cover art by Demin Lu – MolGraphics (www.molgraphics.com)

Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 26

4/5 Inspired also by the poem from Carlos Drummond de Andrade
“A flower rose from the street!
[…] breaks the asphalt […]
I assure you that a flower rose
Its color is unnoticed
Its petals don’t open
Its name is not in the books […] but it is truly a flower!”

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 26

3/5 From this orchestrated bloom, vibrant, mature petals - fat cells in their fullest form - unveil themselves like a masterpiece of adipogenesis.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 26

2/5 These blossoms emerge as a pool of heterogeneous progenitor leaves and preadipocyte buds. Bathed in the essence of Rosiglitazone, the immature petals slowly unfurl, by the crescendo of ribosomal protein expression within the flower’s nuclear heart.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 26

Fat Cells in Full Bloom!
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.

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Reposted by Mirian Krystel De Siqueira

Laura Hulea @laurahulea.bsky.social · Nov 26

Congratulations Mirian @desiqueiramk.bsky.social , the Villanueva and Yang labs at UCLA! Happy to have contributed to such beautiful work highlighting the role of mRNA translation in adipocyte biology.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

5/5: A big thank you to our funding sources for making this research possible @americanheart.bsky.social #UCLA #UCLAIBP - your support is invaluable in advancing our understanding of adipose biology and metabolic health!

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

4/5: Finally, we show how rosiglitazone treatment enhances translation in adipose progenitors, promoting adipogenesis. Blocking this effect via eIF4A inhibition reverses the benefits, highlighting translation remodeling as a key to adipose tissue health in obesity.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

3/5: We discovered that PPARγ-driven ribosomal remodeling enhances adipocyte progenitor and preadipocyte differentiation potential. This effect is directly linked to PPARγ binding to ribosomal genes and is crucial for adipogenesis in obesity.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

2/5: Using single-cell RNA sequencing, we mapped how rosiglitazone (a PPARγ agonist) reshapes transcriptional profiles in adipose tissue, shifting obese states to lean-like ones. Key changes include decreased inflammation & increased lipid metabolism and ribosomal activity.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

1/5: Adipose tissue is central to energy balance and metabolic health, but obesity disrupts its adaptability. Our latest study in #CellReports explores how translation in adipose progenitors is regulated, revealing key insights into obesity's impact.

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Mirian Krystel De Siqueira @desiqueiramk.bsky.social · Nov 25

Excited to share our latest work in #CellReports highlighting a PPARγ dependent remodeling of translation machinery in adipose progenitors. Amazing collab between our lab Claudio Villanueva, Xia Yang, and @laurahulea.bsky.social !
#obesity #metabolism #translation
www.cell.com/cell-reports...

PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity

De Siqueira et al. explore the effects of rosiglitazone on adipose stem cell plasticity, showing that PPARγ stimulates expression of ribosomal factors in adipocyte precursors. This leads to translatio...

www.cell.com

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Reposted by Mirian Krystel De Siqueira

Jalees Rehman @jalees.bsky.social · Nov 25

PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity

www.cell.com/cell-reports...

www.cell.com

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Reposted by Mirian Krystel De Siqueira

Craig Kaplan @triggerloop.bsky.social · Nov 21

This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...

Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes

The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....

www.science.org

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Reposted by Mirian Krystel De Siqueira

Keystone Symposia @keystonesymposia.bsky.social · Nov 21

Join our joint meeting on Obesity & Adipose Tissue / MASH! Submit abstracts for short talks by 11:59 PM MST, December 4th. Visit hubs.la/Q02Z4Jlq0 or hubs.la/Q02Z4G770.
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#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis

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Reposted by Mirian Krystel De Siqueira

Joana Silva @thejoanasilva.bsky.social · Nov 24

Are you a scientist working or interested in RNA Translation? Join our starter pack and share ideas, methodologies and tips to advance our knowledge in how life came to be!
#RNA #ribosome

go.bsky.app/VN78eTo

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Matthew Sinton 🏳️‍🌈 @mcsinton.bsky.social · Nov 24

Working on immunometabolism? Or looking for people who do? Then here’s the starter pack for you! 😊 #Immunosky

go.bsky.app/MvxkA4Q

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Kate Ellacott @neuroendo.bsky.social · Nov 16

Sharing again as I have added a at least 20 newbies and people how I just found out were here. Just need to get that Giles Yeo over here now go.bsky.app/NRoqMvp

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