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brozlab.bsky.social
Broz Lab
@brozlab.bsky.social
220 followers 120 following 14 posts
Lab of Petr Broz @ UNIL. Cell death, innate immunology and pathogen defense
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · 14d
The second day of the @dib-unil.bsky.social #PRR symposium kicks off with more excellent talks including our own Jakub Began.
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dib-unil.bsky.social
Department of Immunobiology at UNIL @dib-unil.bsky.social · 15d
Kicking off the 2nd Symposium on the Immunobiology of Pattern Recognition Receptors organized by our department in Lausanne!

@brozlab.bsky.social @martinon.bsky.social @rebsamenlab.bsky.social @fbm-unil.bsky.social
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rebsamenlab.bsky.social
Rebsamen Lab @UNIL @rebsamenlab.bsky.social · 15d
Full room at the start of the 2nd Immunobiology symposium on pattern recognition receptors!
Looking forward to the great programme that we lined up for you together with @brozlab.bsky.social @martinon.bsky.social

#ImmunoSky
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eckerleisabella.bsky.social
Isabella Eckerle @eckerleisabella.bsky.social · Jul 7
Are you an excellent researcher in the field of genomics of infectious diseases of intracellular pathogens (viruses, intracellular parasites or bacteria)? The apply for this chair of excellence at the University of Geneva, Faculty of Medicine:
jobs.unige.ch/www/wd_porta...
Professeur-e ordinaire, professeur-e associé-e (6460)
en génomique des maladies infectieuses intracellulaires Chaire dexcellence / Full Professor or Associate Professor in Genomics of Intracellular Infectious Diseases Chair of Excellence
jobs.unige.ch
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jul 19
Congrats to @malvinapiz.bsky.social and @inflammasomelab.bsky.social on their new paper on the role of cardiolipin in non canonical inflammasome activation. We’re happy to have contributed to the story 🎉🦠!!

www.embopress.org/doi/full/10....
Cardiolipin inhibits the non-canonical inflammasome by preventing LPS binding to caspase-4/11 | The EMBO Journal
imageimageAvailable caspase-4/11 (CASP4/11) inhibitors also block the activity of caspase-1 (CASP1), which complicates interpretation of results in functional studies and limits their clinical potenti...
www.embopress.org
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jul 19
Here is the latest review from the lab on co infections 🥳. Following up on Philipp’s previous work, Philipp and Petr address what is known and all the open questions in this field :

www.embopress.org/doi/full/10....
Molecular mechanisms of co-infections | EMBO reports
EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.
www.embopress.org
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jun 24
A big congratulations to Dr. Alexandra Boegli for very successfully defending her PhD on listeria activation of the elusive NLRP6! Very well deserved and very well celebrated! 🥳
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Feb 26
Registration opens soon for the second Pattern Recognition receptor symposium in Lausanne Switzerland in October 2025. Check out the link for the great list of speakers.

wp.unil.ch/immunobiolog...
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Feb 25
Congrats to lab member Elliott for winning the poster prize at the Swiss LS2 conference for his work on the activation mechanism of Ninj1 during cell death!! 🥳 @dib-unil.bsky.social @lifesciswitzerland.bsky.social
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
So, NLRP6 can initiate a canonical inflammasome and induces pyroptosis, establishing NLRP6 as a sensor for damage to the endolysosomal system.
brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
Finally, sterile endolysosomal damage alone, using the lysosome rupturing compound LLOME, is sufficient to activate NLRP6.
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
On the bacterial side, bacterial PAMPs aren’t sufficient to activate NLRP6. However, cell to cell spread is important for activation as a mutant deficient for making actin tails is a worse activator of NLRP, as is the plcB mutant deficient in escape from a secondary vacuole.
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
By comparing NLRP6 and 3, they map the upstream region of the NACHT domain - the FISNA and its conserved poly basic motifs- as being important for receptor activation, but despite sequence similarities they show that each receptor is activated by distinct ligands.
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
Then they show that listeria based activation of NLRP6 in HIEC intestinal epithelial cells has all the hallmarks of canonical inflammasome activation including GSDMD cleavage.
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
First they show that WT listeria - but not the dhly mutant which doesn’t express the pore forming toxin Listeriolysin O that allows the bacteria to escape from endolysosomes - are sensed in a HEK reconstitution assay.
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Jan 28
Check out our latest pre print! Alexandra and colleagues show that the enigmatic inflammasome receptor, NLRP6 is activated by sterile and pathogen induced endolysosomal damage. Hot off the press!

www.biorxiv.org/content/10.1...
Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells
NLRP6 controls host defense against bacteria and viruses in the gastrointestinal tract by a poorly understood mechanism. Here, we report that NLRP6 forms an inflammasome upon endolysosomal damage caus...
www.biorxiv.org
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rebsamenlab.bsky.social
Rebsamen Lab @UNIL @rebsamenlab.bsky.social · Dec 10
Very happy to share our latest work just published in @jexpmed.bsky.social! #Firstpost

rupress.org/jem/article/...

Our amazing PhD student @lbernaleau.bsky.social shows that CCDC134 controls TLR biogenesis through the ER-chaperone Gp96. #ImmunoSky #innateimmunity #inflammation

Quick summary here:
CCDC134 controls TLR biogenesis through the ER chaperone Gp96 | Journal of Experimental Medicine | Rockefeller University Press
A loss-of-function genetic screen identifies the ER-resident protein CCDC134 as an essential regulator of Toll-like receptor (TLR) responses. CCDC134 binds
rupress.org
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inflammasomelab.bsky.social
Inflammasome Lab @inflammasomelab.bsky.social · Dec 9
Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells
Nature Immunology - Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate...
rdcu.be
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dib-unil.bsky.social
Department of Immunobiology at UNIL @dib-unil.bsky.social · Dec 9
New manuscript by Sina Bohnacker and Julia Esser-von Bieren published in Science Immunology!

www.science.org/doi/10.1126/...
A helminth enzyme subverts macrophage-mediated immunity by epigenetic targeting of prostaglandin synthesis
H. polygyrus glutamate dehydrogenase drives parasite chronicity by epigenetically and metabolically reprogramming host macrophages.
www.science.org
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lozanzi.bsky.social
Ivan Zanoni @lozanzi.bsky.social · Dec 5
#NoTimeToDie! @inflammasomelab, Holley, Monteleone &co show @natimmunol.bsky.social that cells that die by #pyroptosis, or #necroptosis, are crowned with F-actin filopodia that makes them visible by dendritic cells via CLEC9A to initiate adaptive immunity! www.nature.com/articles/s41...
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells - Nature Immunology
Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate dendritic cells.
www.nature.com
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brozlab.bsky.social
Broz Lab @brozlab.bsky.social · Dec 4
First real bluesky post 🥳 Did you know that NINJ1 exists as an autoinhibited dimer in the PM of cells? In a News & Views, @elliottbernard.bsky.social and Petr describe new results from Dixit & Deshpande, including a resting structure of NINJ1 with 3 helices! Check it out here: rdcu.be/d2nqH
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drnatsampaio.bsky.social
Natalia Sampaio @drnatsampaio.bsky.social · Nov 16
I’ve made a Starter Pack of #innateimmunity and #inflammation researchers to follow! Please contact me to get added or to suggest others to add. We are just getting started 😎
#immunosky
#academicchatter
go.bsky.app/QqJ9AgB
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protinnimmunity.bsky.social
Proteases and Innate immunity lab @ York @protinnimmunity.bsky.social · Nov 18
go.bsky.app/221DCXf
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