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Sebastian S. Cocioba

@atinygreencell.bsky.social

8.6K followers 6.6K following 3.3K posts

Amateur 🌿 Biologist • Aspiring 🌻 Designer Independent Researcher at Binomica Labs Degreeless Heathen • #diybio • #homelab Open Lab Notebook: tinyurl.com/ATGCFFE YouTube Channel: https://youtube.com/@atinygreencell

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Sebastian S. Cocioba @atinygreencell.bsky.social · Jul 8

Excited to announce some new plasmid offerings in my shop at atinygreencell.com! Here's a thread of the current catalog. All proceeds directly support my lab and my continued effort to build useful open-source tools. Feel free to get in touch if you want to know more. Please share! 💚

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Sebastian's Biotech Bazaar

Designer of fine, hand-crafted, bespoke, and fully open source biotechnology tools for educators and researchers. Plasmids, lab equipment, cell lines, and seeds. An ever-growing collection of high qua...

ATinyGreenCell.com

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Sebastian S. Cocioba @atinygreencell.bsky.social · 6h

Will do all kinds of fun stuff once I know this exonic fragment can yield decent DICER bits. I had to make 2 point mutations to make them clonable but ample 21mers that are perfect to make up for it, and none of the mutations are in the seed or core region (2-8 and 9-11bp respectively) so should wrk

Sebastian S. Cocioba @atinygreencell.bsky.social · 6h

Alright, first RNAi construct is designed! This will target my Black Prince snapdragon's chalcone synthase gene, which would render an anthocyanin-less phenotype if silencing worked. I even made a template for designing primers to link to the PDK intron. Here's the plan:

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Sebastian S. Cocioba @atinygreencell.bsky.social · 15h

300bp, and yeah its unique to just chalcone synthase. Exon 2 has regions that are not shared by other kinases. Triple checked :)

Sebastian S. Cocioba @atinygreencell.bsky.social · 1d

Making some RNAi constructs. That long red stick was perfect binding to make that dsRNA sandwich that DICER needs. Fun!

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Sebastian S. Cocioba @atinygreencell.bsky.social · 1d

Another round of petunia leaf disk transformation attempts. This time co-culture buffer was just liquid MS and then plated onto MS with hormones and meropenem 100mg/L to clear the agro. Let's see if those rings of death appear again...

Sebastian S. Cocioba @atinygreencell.bsky.social · 1d

No one likes snobs. All are welcome 💚

Sebastian S. Cocioba @atinygreencell.bsky.social · 2d

Blargggh, big plant breeder i was gonna do work for is in a merger so no luck on contract.

Reposted by Sebastian S. Cocioba

Nick Desnoyer @nickdesnoyer.bsky.social · 2d

Friday Flower 007: Petunia 💮✨

Petunias carry the active transposon dTph1, which powers mutant screens and paints stripey patterns as it jumps.

Their five petals are fused, with diverse color designs tracing the corolla’s fusion seam.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 3d

No Ai image gen I've tried can modulate snapdragon petal morphology. They have no idea whats possible. We don't either, for the most part, but a tool set to be "ultimate creativity" is utterly derivative.

It either make photorealism or nonsense and nothing in between.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 3d

Another concept I want to dive deeper into is the organism as a superposition of an inherited past and multiplicity of unrealized futures. Visually showcasing the landscape of potential will be difficult but worth it.

Lots of hard analogs to my own life navigating failings.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 3d

There is this really aesthetic parallel between film photography and plant breeding I didnt notice until just now: the latent image

Silver grains capture starlight into an image that needs to be developed to be seen.

Plant breeding captures latent phenotype only seen in progeny

a canon camera with a floral design on it

ALT: a canon camera with a floral design on it

media.tenor.com

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Sebastian S. Cocioba @atinygreencell.bsky.social · 3d

Writing my first art grant applicaton for my flower work! It wont be much but it will open doors to future funding, and its not from the NEA so dont need to focus on weird trumpian asks.

There will be flowers for everyone.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 4d

My entire uncareer. All of it.

Conrad Hackett @conradhackett.bsky.social · 5d

Has anything great happened in your life because of social media?

Sebastian S. Cocioba @atinygreencell.bsky.social · 4d

Why is anyone losing? I thought the whole point of Ai was infinite abundance? Should this not be the end of competition and resource scarcity?

🙃

Sebastian S. Cocioba @atinygreencell.bsky.social · 4d

Makes sense! Im thinking of just subdividing it as a CDS with N term, linker, and C term overhang grammar. That way the ends are still harmonious with all my golden braid promoters and terminators :)

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Reposted by Sebastian S. Cocioba

Urszula McClurg @urszulamcclurg.bsky.social · Mar 2

Another website worth checking is the jobrxiv.org which is great for PIs (preprint principles, free to post) and is becoming increasingly popular

Science Jobs - Find science and research jobs

The international job board for scientists, by scientists. Find science jobs in academia or industry: MSc, PhD, Postdoc, Scientist, Faculty and more!

jobrxiv.org

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Sebastian S. Cocioba @atinygreencell.bsky.social · 4d

Gonna explore some RNAi constructs since I know for a fact those are 100% off-patent and can help confirm some gene model hypotheses with less moving parts.

1. Sense-Intron Spacer Hairpin-Antisense.
2. Make dsRNA sandwich.
3. Done.

Good enough for a Nobel = good enough for me.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 4d

Genuinely my favorite email to read first thing in the morning. All the new plant expression plasmids I built came back perfect.

Golden Braid remains undefeated.

Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

Chammomile is so weird.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

She did an interview with me :)

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Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

Really well! Just need to select for slightly shorter floral spikes; snaps often lean toward 18" at maturity but a few here are pushing almost 2ft!

Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

I love my flowers

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Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

a man in a tuxedo and bow tie is sitting in an auditorium and giving a thumbs up .

ALT: a man in a tuxedo and bow tie is sitting in an auditorium and giving a thumbs up .

media.tenor.com

Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

The interesting part is that both the wild type and agro disks seem to do this, with total necrosis a week later. For a good negative control, I treat the wild type disks the sane way as the agro i.e. soak in same buffer, so maybe its the buffer surfactant? The plot — she thickens.

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Sebastian S. Cocioba @atinygreencell.bsky.social · 5d

The last two petunia leaf disk transformation have this spot of death in the center. Overbleaching? Necrosis signal bc disk too small? Gonna do some tests to see whats causing this. First guess is the co-culture buffer. Let's find out!

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