Lightnews — Scholar-powered news

Amir Rahmani @astormic.bsky.social · 24d

But does it have to be H-1B visa?

Reposted by Amir Rahmani

Kari Herrington @kahsage.bsky.social · 27d

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

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Amir Rahmani @astormic.bsky.social · Sep 13

a black and white drawing of a cat 's eyes in the dark

ALT: a black and white drawing of a cat 's eyes in the dark

media.tenor.com

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Amir Rahmani @astormic.bsky.social · Aug 30

Thank you! 🤓

Amir Rahmani @astormic.bsky.social · Aug 28

Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.

doi.org/10.1101/2025...

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Amir Rahmani @astormic.bsky.social · Aug 27

I’ve never had that privilege!

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Amir Rahmani @astormic.bsky.social · Aug 27

I really need a keyboard blocker like this!

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Amir Rahmani @astormic.bsky.social · Aug 27

Huge thanks to an incredible team who made this possible 🙌 especially Aleks for his great supervision and support.

Amir Rahmani @astormic.bsky.social · Aug 27

We believe smFC brings single-molecule sensitivity to scalable cell analysis.
Excited to see how this impacts biology & medicine!
Read the full story here 👇
www.biorxiv.org/content/10.1...

Single-molecule flow cytometry

Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...

www.biorxiv.org

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Amir Rahmani @astormic.bsky.social · Aug 27

We applied smFC to triple-negative breast cancer (TNBC) cells:
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.

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Amir Rahmani @astormic.bsky.social · Aug 27

With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail

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Amir Rahmani @astormic.bsky.social · Aug 27

smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.

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Amir Rahmani @astormic.bsky.social · Aug 27

Conventional flow cytometry is powerful but has a blind spot:
❌ It can’t detect proteins expressed at very low levels (<100–1000 molecules/cell).
That means many weak but biologically crucial signals are invisible.

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Amir Rahmani @astormic.bsky.social · Aug 27

👀 What if your flow cytometer could see the invisible?

Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...

Single-molecule flow cytometry

Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...

www.biorxiv.org

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Reposted by Amir Rahmani

Tanner Fadero @tanner-fadero.bsky.social · Aug 19

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

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Reposted by Amir Rahmani

Brian Northan @truenorth-ia.bsky.social · Aug 16

I was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?

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Amir Rahmani @astormic.bsky.social · Aug 8

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Reposted by Amir Rahmani

Siân Culley @superresolusian.bsky.social · Aug 7

Hello! Very excited to share our latest preprint, which is great news for us but terrible news for any diehard fans of PSNR and SSIM as image quality metrics in microscopy... (1/5)
www.biorxiv.org/content/10.1...

Image quality metrics fail to accurately represent biological information in fluorescence microscopy

Image processing methods offer the potential to improve the quality of fluorescence microscopy data, allowing for image acquisition at lower, less phototoxic illumination doses. The training and evalu...

www.biorxiv.org

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Amir Rahmani @astormic.bsky.social · Jul 21

That makes perfect sense!

Amir Rahmani @astormic.bsky.social · Jul 21

Excellent work Johannes!

Would SST work for long docking strands (70–80 nt, like in DNA-PAINT SPT)? I believe ROS-induced damage is the primary cause of degradation in longer strands too, but they may be more vulnerable.

Also, does sodium sulfite affect buffer viscosity or refractive index?

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Amir Rahmani @astormic.bsky.social · Jul 11

Feedback mechanisms involving p21-activated kinase enzymes underlie excitability of a cell's actin scaffold.

journals.biologists.com/jcs/article/...

A role for class I p21-activated kinases in the regulation of the excitability of the actin cytoskeleton

Highlighted Article: Inhibition of class I p21-activated kinase activity leads to the constitutitive production of PI(3,4,5)P3 at the plasma membrane and a dysregulation of the actin cytoskeleton.

journals.biologists.com

Amir Rahmani @astormic.bsky.social · Jul 10

I can't imagine there is any other way!

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Reposted by Amir Rahmani

Christof Gebhardt @gebhardtlab.bsky.social · Jun 27

The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...

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Reposted by Amir Rahmani

Jon Daniels @jsdaniel02.bsky.social · Jul 9

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

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Reposted by Amir Rahmani

Genes & Development @genesdev.bsky.social · Jul 8

📽️ G&D Tapes 📽️

G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social

Read the full story here:
➡️ tinyurl.com/gd352899

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