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Reposted by Arif Ashraf

Nat Prunet @nat-prunet.bsky.social · 1h

Grateful to see one my #superresolution #pollen images won 2nd place at the 2025 @bioimagingna.bsky.social image contest!

www.bioimagingnorthamerica.org/image-contes...

#microscopy #confocal #botany #plantscience #allergies #bioimaging

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Arif Ashraf @aribidopsis.bsky.social · 2h

🔬🍀

Mind blowing live cell imaging by Vivek

Look at these beautifully radiating Hechtian Strands, visualized using GFP: RabA2b.

Novel insights into RabA2b intracellular localization suggest a role in plasmodesmatal function @frontiersin.bsky.social

www.frontiersin.org/journals/pla...

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Arif Ashraf @aribidopsis.bsky.social · 17h

It's Thanksgiving in Canada today!

Every year, I ask myself same question, what I am thankful for?

I am thankful for having a bench in the lab and doing some experiments everyday. Here are positive plants (attached picture) from my screening.

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Reposted by Arif Ashraf

David Lowry @davidlowry.bsky.social · 1d

Excited by this breakthrough work led by Dr. Lauren Stanley (now Assistant Professor at Central Michigan University). Lauren was able to develop for the first time floral dip/spray transformation methods in the yellow monkeyflower (Mimulus guttatus). bsapubs.onlinelibrary.wiley.com/doi/10.1002/...

Just add water: A simple floral bud injection method for stable Agrobacterium‐mediated transformation in two ecotypes of Mimulus guttatus

Premise Stable transformation is the biggest barrier to studying gene function in plants. In most species, transformation requires tissue culture and regeneration methods that may be arduous and cau...

bsapubs.onlinelibrary.wiley.com

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Arif Ashraf @aribidopsis.bsky.social · 1d

🍀🔬

E69K mutation in β-tubulin 2 blocks cell wall integrity signaling during plant cell elongation @emboreports.org

www.embopress.org/doi/full/10....

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Greg Vert @plantubiquitin.bsky.social · 1d

Great work by julien Spielmann now published in @theplantjournal.bsky.social.
onlinelibrary.wiley.com/doi/10.1111/...

Canonical tyrosine‐based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake

Control of IRT1 cell surface levels is critical for plant iron uptake from the soil and was previously shown to be regulated by ubiquitin-mediated endocytosis upon non-iron metal excess. Here, we she...

onlinelibrary.wiley.com

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Cytoskeleton Journal @cytoskeljournal.bsky.social · 1d

The new issue of #CYTOSKELETON is now out! You can check out the issues here: onlinelibrary.wiley.com/toc/19493592... #microscopymonday

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Keith Duncan @cygnusplantxray.bsky.social · 4d

I'm frequently asked if we can use XRM imaging to study Arabidopsis, and the answer is absolutely yes. The pollen grains were segmented using basic grayscale thresholding. This scan used the 4X lens, stayed tuned for results from the 20X.
@danforthcenter.bsky.social
@zeiss-microscopy.bsky.social

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Reposted by Arif Ashraf

Michael Raissig @michaelraissig.bsky.social · 4d

Using cell wall immunostainings, Alex found differential staining of LM6, which stains arabinans. B. distachyon showed strong staining in subsidiary cells, and this staining was absent in B. stacei.

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Michael Raissig @michaelraissig.bsky.social · 4d

Anna therefore looked at the subcellular features of these stomatal complexes using TEM and found no striking differences between B. stacei and B. distachyon. Nonetheless, Anna saw the huge symplastic connections between the grass guard cells and shared vacuoles (a feature specific to grass stomata)

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Michael Raissig @michaelraissig.bsky.social · 4d

Our paper comparing stomata of the three annual Brachypodium species is out 🌾
bmcplantbiol.biomedcentral.com/articles/10....
A great collaborative effort between @lbmountain.bsky.social, & our friends at University of Silesia (Alexander Betekhtin and Anna Milewska-Hendel). Funded by @dfg.de and NAWA

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Michael Raissig @michaelraissig.bsky.social · 1d

Happy to share our recent thoughts on how grasses form and pattern stomata
doi.org/10.1016/j.pb...
🌾From fate establishment, through within-file patterning to dumbbell formation🌾
Congrats to
@lbmountain.bsky.social
on getting two publications out in a single day - a first for the lab!

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Arif Ashraf @aribidopsis.bsky.social · 3d

ha ha 😀yes and, some fancy equipment

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Arif Ashraf @aribidopsis.bsky.social · 3d

Thanks, Tatsuya 😀

Arif Ashraf @aribidopsis.bsky.social · 4d

Thanks 😀🌱🔬

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Arif Ashraf @aribidopsis.bsky.social · 4d

Thanks, Sean 😀

Arif Ashraf @aribidopsis.bsky.social · 4d

Thanks, Sam 😀

Arif Ashraf @aribidopsis.bsky.social · 4d

Massive news for the lab 🎉🎉🎉

We received Canada Foundation for Innovation - CFI's JELF (John R. Evans Leaders Fund) grant!

Thanks to the team, office of research @science.ubc.ca, and colleagues @ubcbotany.bsky.social for the generous help and support.

www.innovation.ca/news/jelf-cf...

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Yale MCDB @mcdb-yale.bsky.social · 5d

🌿 Calling All Plant Enthusiasts! 🌺

Join us at the Sussex Symposium this Friday for a day dedicated to plant biology! 🌳

🎙️ Don't miss the keynote by Dr. Ken Birnbaum!

🗓️ 9 AM - 5 PM 📍 Jones Auditorium, Connecticut Agricultural Experiment Station

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Raju Soolanayakanahally @rajuaafc.bsky.social · 5d

Breeding better #seabuckthorn! At AAFC, we identified 4 standout genotypes with longer fruit stalks (easier to harvest), larger, sweeter berries, and richer nutrition. Weather played a key role too. doi.org/10.1139/cjps...

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Sam Connell @samuelconnell.3i.social · 5d

....and a 3i customer visiting Heidelberg from Australia kindly sent me this photo of Nicole and @dvonwangenheim.bsky.social busy talking about microscopy with a handful of visitors to our booth.

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MSU Plant Resilience Institute @msupri.bsky.social · 6d

We're excited to welcome Christopher Topp to the Plant Resilience Institute! Dr. Topp uses powerful imaging tools to envision the future of sustainable agriculture, and he will soon bring his expertise in root biology to MSU. Read the full story: plantresilience.msu.edu/pri-news/202...

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Carolyn Rasmussen @carolynplants.bsky.social · 7d

Excited to share this preprint on the microtubule severing protein Katanin, and its role in cell elongation and division in maize. First author Stephanie Martinez worked collaboratively with many folks to bring you this preprint. Check it out! 🔬🌽 www.biorxiv.org/content/10.1...

KATANIN promotes cell elongation and division to generate proper cell numbers in maize organs

Microtubule severing is essential for proper eukaryotic cell elongation and division. Here we show that the microtubule severing protein, KATANIN, is encoded by two genes in Zea mays (maize) called Di...

www.biorxiv.org

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Liz Haswell @ehaswell.bsky.social · 7d

I started to work on MscS-Like proteins 20 years ago, dreaming that they were involved in plant mechanotransduction. And it is becoming more & more clear that they are!
#plantscience 🧪
"DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."

Arif Ashraf @aribidopsis.bsky.social · 13d

🍀🔬

MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap @natcomms.nature.com from Toyota lab.

www.nature.com/articles/s41...

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Carolyn Rasmussen @carolynplants.bsky.social · 7d

A time projection of the microtubules in the katanin mutant by Stephanie Martinez 🥳

Maize epidermal cells with a live cell marker for microtubules where microtubule color from cyan to red indicate time.

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