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aaandmoore.bsky.social
Andy Moore
@aaandmoore.bsky.social
2.4K followers 1.2K following 71 posts
User of microscopes. Interested in organelles and how they move. Husband, dad, intermediate filament apologist, and postdoc in the JLS lab at HHMI Janelia Research Campus.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Apr 22
Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.

www.biorxiv.org/content/10.1...
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Reposted by Andy Moore
jcb.org
Journal of Cell Biology @jcb.org · 22d
📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti...

#Euro-IF
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Reposted by Andy Moore
ibudin.bsky.social
Itay Budin @ibudin.bsky.social · Sep 15
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology
An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...
www.nature.com
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Aug 19
Thanks! This was actually all dyes. Mito tracker green, SPY555-Tubulin, and SPY650-FastAct-X.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Aug 18
Whoops forgot to add that - 5 microns
aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Aug 18
It’s sped up - the timer is minutes:seconds
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Aug 18
This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.
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Reposted by Andy Moore
christlet.bsky.social
Christophe 🔬 L @christlet.bsky.social · Jun 14
Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...
Continuous self-repair protects vimentin intermediate filaments from fragmentation | PNAS
Intermediate filaments are key regulators of cell mechanics. Vimentin, a type of intermediate filament expressed in mesenchymal cells and involved ...
doi.org
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
I double checked the metadata and I’m 90% sure it’s not the sun.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
Zeiss 880 w/ airyscan
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
Not discussed so much but those tubules pressed up against the nuclear envelope are certainly unique.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
Thank you! This is an airyscan stack.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
Thank you! Yup, good old COS-7.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Jun 11
This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.
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Reposted by Andy Moore
aicjanelia.bsky.social
AIC at Janelia @aicjanelia.bsky.social · Jun 6
Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).
aicjanelia.bsky.social
AIC at Janelia @aicjanelia.bsky.social · Apr 15
The latest AIC Call for Proposals is now open! Deadline is June 16, 2025. Come use @hhmijanelia.bsky.social imaging technology and expertise for FREE. Visit www.aicjanelia.org/apply for details. Before applying, contact us to schedule a technical consultation!
Apply | aicjanelia
Apply to use the advanced microscopes housed at the AIC.
www.aicjanelia.org
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 30
Is it just me or do these cells look a lot like the americas?
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 25
This is a HeLa
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 25
Close! This is a HeLa expressing lifeact-egfp. It’s an odd looking hela to be fair
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Reposted by Andy Moore
aafkegros.bsky.social
Aafke Gros @aafkegros.bsky.social · May 23
In the context of our @reviewcommons.org revision process, I'm happy to announce Microscopy Nodes v2.2.0!
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...
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Reposted by Andy Moore
healthcare.nikon.com
Nikon Microscope Solutions @healthcare.nikon.com · May 14
📣 We’re thrilled to announce the judging panel for the 2025 #NikonSmallWorld photomicrography and Small World in Motion video competitions!

🔗 Click to learn about this year's judges: bit.ly/43hmGBq

#Microscopy #Imaging #SciComm #SciArt
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 25
Actin sun.
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 25
FilaBuster - Vimentin IF fragmentation
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Reposted by Andy Moore
loxstoplox.bsky.social
Blake Hernandez @loxstoplox.bsky.social · May 23
In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.
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Reposted by Andy Moore
loxstoplox.bsky.social
Blake Hernandez @loxstoplox.bsky.social · May 23
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · May 3
Congratulations!!!
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aaandmoore.bsky.social
Andy Moore @aaandmoore.bsky.social · Apr 22
Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.

www.biorxiv.org/content/10.1...
2 36 120