Links for you. Science: Megaplasmids associate with Escherichia coli and other Enterobacteriaceae (really interesting) In 2022, the world axed a disease name seen as racist. US just switched back Mom's COVID Shot Aided Baby, Too, Large Review Confirmed Long COVID is here to stay—even in children (paper here) What Is Thirst? Lauer is super concerned about the demoralizing impact of low success rates…now that it is white folks being affected…

A total of 73 <i>Enterobacteriaceae</i> strains were isolated, including <i>Klebsiella pneumoniae</i>, Salmonella enterica, <i>Enterobacter hormaechei</i>, and <i>Escherichia coli</i>. Resistance rates to tetracycline, ciprofloxacin, nalidixic acid, sulfamethoxazole-trimethoprim, and ampicillin exce …

Research reveals beneficial microbes inside <em>Panax japonicus</em> seeds could boost germination and plant health. Seven fungi & three bacteria were isolated, with <em>Enterobacteriaceae</em> &…

Algorithmic methods to antibiotic discovery are reaching a state of performance that can meaningfully influence how novel antibacterial molecules are discovered.

Enterololin is a narrow-spectrum antibiotic that selectively kills Enterobacteriaceae in vitro and in a mouse model of adherent-invasive Escherichia coli gut infection. AI-guided mechanism-of-action studies identified LolCDE as its molecular target.

Humans and animals are ubiquitously colonized by Enterobacteriaceae , a bacterial family that contains both commensals and clinically significant pathogens. Here, we report Enterobacteriaceae megaplasmids of up to 1.58 Mbp in length in infant and adult guts, and other microbiomes. Of the 19 complete plasmid genomes, one was reconstructed from an Escherichia coli isolate; others were linked to species of Citrobacter and Enterobacter via analysis of genome modification patterns. The detection of related plasmids in different Enterobacteriaceae , conjugation machinery, and more diverse modified motifs in certain plasmids compared to hosts suggests that these elements are self-transmissible, with a broad host range. The plasmids encode multi-drug efflux systems and potential secreted effectors. Up to 208 tRNAs are encoded, and include sequence variants that may counter tRNA-centric defense mechanisms. Overall, the vast megaplasmid coding capacity may broaden host range, increase competitiveness, control invasion by other elements, and counter programmed cell death. ### Competing Interest Statement The authors have declared no competing interest. National Institutes of Health, https://ror.org/01cwqze88, 5R01AI092531 Swiss National Science Foundation, IZJFZ3-177614 Biotechnology and Biological Sciences Research Council, https://ror.org/00cwqg982, BB/X011011/1, BBS/E/QU/230002A, BB/T008717/1 Chan Zuckerberg Initiative (United States), https://ror.org/02qenvm24 Finnish Multidisciplinary Centre of Excellence in Antimicrobial Resistance Research, 346127