Peter Wang
@wyppeter.bsky.social
Postdoc at Doudna Lab (UC Berkeley). Biochemical + biophysical principles of protein/RNA mechanisms. Previously PhD at Bartel lab (MIT/Whitehead).
In this updated paper, we further described conformational changes required for catalysis, and a network of interactions near the active site that support slicing. Read more here: tinyurl.com/AGO2-slicing
The structural basis for RNA slicing by human Argonaute2
Mohamed et al. report the cryoelectron microscopy structure of human AGO2 with fully paired guide RNA. Their analysis reveals the structural basis for the slicing activity that drives RNAi, showing that the slicing-competent conformation is achieved by domain movements and RNA-protein contacts distinct from those of conformational intermediates and prokaryotic homologs.
tinyurl.com
January 1, 2025 at 6:31 PM
In this updated paper, we further described conformational changes required for catalysis, and a network of interactions near the active site that support slicing. Read more here: tinyurl.com/AGO2-slicing
More than five years ago, we charted our plans to unravel the biochemistry and biophysics of the AGO2 enzyme, and the eventual goal of solving its catalytic structure. It has been a great journey ever since, and we are excited to finally share these results.
January 1, 2025 at 6:31 PM
More than five years ago, we charted our plans to unravel the biochemistry and biophysics of the AGO2 enzyme, and the eventual goal of solving its catalytic structure. It has been a great journey ever since, and we are excited to finally share these results.