Reposted by Vincent Spreng
Excited to share my main PhD work is finally out! 🥳
We built a phage-assisted evolution platform to evolve allosteric protein switches (POGO-PANCE) and introduce cumulative, targeted mutations/Indels (RAMPhaGE)!
See how we evolved ultra-strong, light-switchable AraC variants 🌚🌞
We built a phage-assisted evolution platform to evolve allosteric protein switches (POGO-PANCE) and introduce cumulative, targeted mutations/Indels (RAMPhaGE)!
See how we evolved ultra-strong, light-switchable AraC variants 🌚🌞
Phage-Assisted Evolution of Allosteric Protein Switches https://www.biorxiv.org/content/10.1101/2025.06.12.659331v1
June 13, 2025 at 9:23 AM
Excited to share my main PhD work is finally out! 🥳
We built a phage-assisted evolution platform to evolve allosteric protein switches (POGO-PANCE) and introduce cumulative, targeted mutations/Indels (RAMPhaGE)!
See how we evolved ultra-strong, light-switchable AraC variants 🌚🌞
We built a phage-assisted evolution platform to evolve allosteric protein switches (POGO-PANCE) and introduce cumulative, targeted mutations/Indels (RAMPhaGE)!
See how we evolved ultra-strong, light-switchable AraC variants 🌚🌞
Reposted by Vincent Spreng
Some exciting results to share! The MagLOV and related proteins from @andrewgyork.bsky.social and @mariaingaramo.bsky.social are quantum. We measured 4% optically detected magnetic resonance contrast in living bacterial cells at room temperature. www.biorxiv.org/content/10.1...
![A. Structure of AsLOV2 [PDB 2V1A] with mutations resutling in MagLOV~2 highlighted. For the first time, quantum sensing in living cells using a fluorescent protein-based sensor can be performed by driving spin transitions using radio-frequency (RF) fields in the presence of a static magnetic field. B. Simplified photocycle diagram in the case of a large external magnetic field.
C. Microscope image cropped to a single cell expressing MagLOV 2. Scalebar is 1 um.
D. For MFE measurements the magnetic field was modulated between 0 mT and 10 mT, here with a period 10 seconds. The intensity over time is integrated over pixels covering the single cell, with an exponential decay (due to photobleaching) removed.
E. A single cell expressing MagLOV 2 displaying an ODMR signal 4% contrast.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:tludcv74nxumusfwzkmfcl5q/bafkreigmpmmy3xz6alxsxwwmsw4dxn7lkebjl4lqtcwvpiljdiubztlmqa@jpeg)
November 28, 2024 at 3:13 PM
Some exciting results to share! The MagLOV and related proteins from @andrewgyork.bsky.social and @mariaingaramo.bsky.social are quantum. We measured 4% optically detected magnetic resonance contrast in living bacterial cells at room temperature. www.biorxiv.org/content/10.1...