@sevaplasmids.bsky.social
A non-profit Open Access repository of standardised plasmids following the SEVA format. Check out our database and request your #plasmid free of charge #synbio
seva-plasmids.com
seva-plasmids.com
🚨 News on bioremediation! A study published in Nature reveals how by genetically modifying the bacterium Vibrio natriegens, it simultaneously degrades five toxic contaminants in industrial wastewater and saline soils. Read the full article: www.nature.com/articles/d41...
Engineered bacteria can degrade five wastewater pollutants at the same time
Five gene clusters have been introduced into a single rapidly growing bacterial strain.
www.nature.com
November 14, 2025 at 11:02 AM
🚨 News on bioremediation! A study published in Nature reveals how by genetically modifying the bacterium Vibrio natriegens, it simultaneously degrades five toxic contaminants in industrial wastewater and saline soils. Read the full article: www.nature.com/articles/d41...
👩🔬 Want to team up with us to expand the #SEVA collection?
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
November 12, 2025 at 11:01 AM
👩🔬 Want to team up with us to expand the #SEVA collection?
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com
Designing replication sequences is challenging in #SynBio. Researchers at the Innovative Genomics Institute present OriGEN, a language model generating functional plasmids beyond wild type, capturing the complex mechanisms of biological replication.
👉 doi.org/10.1101/2025...
👉 doi.org/10.1101/2025...
Generating functional plasmid origins with OriGen
While generative artificial intelligence has shown promise for biological design, no computational system has yet created sequences proven capable of replication. Focusing on plasmids as minimal…
doi.org
November 10, 2025 at 11:02 AM
Designing replication sequences is challenging in #SynBio. Researchers at the Innovative Genomics Institute present OriGEN, a language model generating functional plasmids beyond wild type, capturing the complex mechanisms of biological replication.
👉 doi.org/10.1101/2025...
👉 doi.org/10.1101/2025...
🚀 Meet Constructive Bio! This biotech turns living bacteria into programmable biofactories creating new classes of polymers. At #SEVA, we love seeing initiatives like this driving innovation in #SynBio. Read more 👉 www.nature.com/articles/s41...
Editor’s pick: Constructive Bio - Nature Biotechnology
Each year, Nature Biotechnology highlights companies that received sizeable early-stage funding in the previous year. Constructive Bio is using synthetic E. coli and non-canonical amino acids to…
www.nature.com
November 7, 2025 at 11:01 AM
🚀 Meet Constructive Bio! This biotech turns living bacteria into programmable biofactories creating new classes of polymers. At #SEVA, we love seeing initiatives like this driving innovation in #SynBio. Read more 👉 www.nature.com/articles/s41...
💭 Imagine #SynBio research where every experiment can be easily replicated and labs collaborate seamlessly. No technical hurdles, just shared progress. That’s our vision at #SEVA! Get your free plasmids and join the movement for #standardisation 👉https://seva-plasmids.com/
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
November 5, 2025 at 11:00 AM
💭 Imagine #SynBio research where every experiment can be easily replicated and labs collaborate seamlessly. No technical hurdles, just shared progress. That’s our vision at #SEVA! Get your free plasmids and join the movement for #standardisation 👉https://seva-plasmids.com/
Pseudomonas chlororaphis is a promising biofactory, but hard to engineer. A new piece of work has characterised its endogenous CRISPR/Cas I–F system and built a precise editing & CRISPRi toolkit, enabling efficient genetic manipulation. Take a look 👉 pubs.acs.org/doi/10.1021/...
October 31, 2025 at 11:02 AM
Pseudomonas chlororaphis is a promising biofactory, but hard to engineer. A new piece of work has characterised its endogenous CRISPR/Cas I–F system and built a precise editing & CRISPRi toolkit, enabling efficient genetic manipulation. Take a look 👉 pubs.acs.org/doi/10.1021/...
Looking for efficient ways to program intercellular communication? 🧬
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...
An M13 phagemid toolbox for engineering tuneable DNA communication in bacterial consortia
Intercellular communication is essential for distributed genetic circuits operating across cells in multicellular consortia. While diverse signalling molecules have been employed—ranging from quorum…
doi.org
October 29, 2025 at 11:03 AM
Looking for efficient ways to program intercellular communication? 🧬
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...
The evolution of #SynBio advances with new regulatory toolboxes 🧬
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...
Frontiers | Genetic circuits in synthetic biology: broadening the toolbox of regulatory devices
Devices sensing inputs and generating outputs are fundamental regulatory units, and as such are the basis of more complex regulatory networks. We provide an ...
doi.org
October 27, 2025 at 11:04 AM
The evolution of #SynBio advances with new regulatory toolboxes 🧬
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...
🌊 Marine bacteria like Pseudovibrio hold enormous ecological & biotech potential, still largely unexplored.✨ Discover the new SEVA-based genetic toolbox developed by researchers at the University of Illinois Chicago — opening ways to study their biology & new applications👉 doi.org/10.1128/aem....
Evaluation of vectors for gene expression in Pseudovibrio marine bacteria | Applied and Environmental Microbiology
The genus Pseudovibrio of α-Proteobacteria has consistently been isolated from marine sponges and other marine organisms such as corals and algae. Pseudovibrio bacteria are a source of antibiotics…
doi.org
October 24, 2025 at 10:02 AM
🌊 Marine bacteria like Pseudovibrio hold enormous ecological & biotech potential, still largely unexplored.✨ Discover the new SEVA-based genetic toolbox developed by researchers at the University of Illinois Chicago — opening ways to study their biology & new applications👉 doi.org/10.1128/aem....
What makes a SEVA plasmid unique?
It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo
With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com
It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo
With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 22, 2025 at 10:02 AM
What makes a SEVA plasmid unique?
It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo
With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com
It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo
With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com
🧬 How many plasmids can a single bacterium carry?
That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...
That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...
How many plasmids can bacteria carry? A synthetic biology perspective | Open Biology
Plasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that…
doi.org
October 17, 2025 at 10:02 AM
🧬 How many plasmids can a single bacterium carry?
That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...
That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...
⚠️ Hold on! Don’t scroll!
Still don't know the basic design of a SEVA plasmid?
🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.
Find your match here 👇
seva-plasmids.com
Still don't know the basic design of a SEVA plasmid?
🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.
Find your match here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 15, 2025 at 10:03 AM
⚠️ Hold on! Don’t scroll!
Still don't know the basic design of a SEVA plasmid?
🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.
Find your match here 👇
seva-plasmids.com
Still don't know the basic design of a SEVA plasmid?
🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.
Find your match here 👇
seva-plasmids.com
Biology isn’t an exact science — but standardisation helps us get closer to reproducibility and clarity.
That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com
That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 13, 2025 at 10:02 AM
Biology isn’t an exact science — but standardisation helps us get closer to reproducibility and clarity.
That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com
That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com
How do you imagine an artificial DNA nucleus? This study presents a synthetic DNA protonucleus that enables localized RNA transcription and condensation into nuclear-like architectures. A novel tool to investigate real nuclear function. Take a look
👉 doi.org/10.1038/s414...
👉 doi.org/10.1038/s414...
Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus
Nuclear biomolecular condensates are functional sub-compartments within the cell nucleus. Here, the authors develop a synthetic DNA protonucleus that enables RNA transcription and condensation into diverse...
doi.org
October 10, 2025 at 10:03 AM
How do you imagine an artificial DNA nucleus? This study presents a synthetic DNA protonucleus that enables localized RNA transcription and condensation into nuclear-like architectures. A novel tool to investigate real nuclear function. Take a look
👉 doi.org/10.1038/s414...
👉 doi.org/10.1038/s414...
🚀 #SEVA proves its potential once again!
@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...
@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...
Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli
A robust in vivo method for engineering plasmid DNA in E. coli using a triple-selection system that works at any copy number, enabling fast, scalable construction of standardized plasmids for synthetic...
doi.org
October 8, 2025 at 10:02 AM
🚀 #SEVA proves its potential once again!
@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...
@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...
⏳ Stop wasting hours searching for the perfect plasmid for your experiment.
👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.
🔬 Open, reliable, and ready for your research.
Check it out here 👇
seva-plasmids.com
👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.
🔬 Open, reliable, and ready for your research.
Check it out here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 6, 2025 at 10:01 AM
⏳ Stop wasting hours searching for the perfect plasmid for your experiment.
👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.
🔬 Open, reliable, and ready for your research.
Check it out here 👇
seva-plasmids.com
👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.
🔬 Open, reliable, and ready for your research.
Check it out here 👇
seva-plasmids.com
📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:
1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes
And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...
1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes
And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...
Scaling laws of bacterial and archaeal plasmids - Nature Communications
The capacity of a plasmid to express genes is constrained by parameters such as its length and copy number. Here, Maddamsetti et al. present a computational method that enables rapid and accurate…
doi.org
October 3, 2025 at 10:02 AM
📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:
1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes
And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...
1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes
And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...
🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455
Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus
Xanthobacter autotrophicus is a metabolically flexible microorganism with two key features: (1) The organism has adapted to grow on a wide variety of carbon sources including CO2, methanol, formate,…
doi.org
October 1, 2025 at 10:03 AM
🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455
📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...
A framework for complex signal processing via synthetic biological operational amplifiers - Nature Communications
Engineering genetic circuits to control gene expression in response to complex signal inputs remains a challenge due to limited precision. This study introduces operational amplifiers that decode…
doi.org
September 29, 2025 at 10:02 AM
📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...
With #SEVAplasmids you’re just 2 steps away from your ideal plasmid!
1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form
That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/
1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form
That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/
CONTACT - SEVA plasmids - Standard European Vector Architecture
Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…
seva-plasmids.com
September 26, 2025 at 10:02 AM
With #SEVAplasmids you’re just 2 steps away from your ideal plasmid!
1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form
That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/
1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form
That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/
🧑🤝🧑CRISPR/Cpf1 × pSEVA: a powerhouse combo in #SynBio! German researchers applied this strategy in Photorhabdus & Xenorhabdus, boosting production of high-value compounds like safracin B, a precursor to the anti-cancer drug ET-743. Read here👉 doi.org/10.1186/s129...
Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling - Microbial Cell Factories
Background Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce…
doi.org
September 24, 2025 at 12:04 PM
🧑🤝🧑CRISPR/Cpf1 × pSEVA: a powerhouse combo in #SynBio! German researchers applied this strategy in Photorhabdus & Xenorhabdus, boosting production of high-value compounds like safracin B, a precursor to the anti-cancer drug ET-743. Read here👉 doi.org/10.1186/s129...
Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research
RESEARCH | Tom Ellis Lab
Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…
www.tomellislab.com
September 22, 2025 at 10:02 AM
Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research
#SynBio never ceases to amaze: a new study has succeeded in increasing energy metabolism in mammalian cells, algae, and fungi through the expression of E. coli LplA, a lipoylation protein. The result? Read here 👉 doi.org/10.1038/s414...
Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein - Nature Communications
Lipoylated proteins are essential for energy metabolism of both prokaryotes and eukaryotes. Here, the authors showed that the E. coli lipoate-protein ligase A (LplA) can boost the lipoylation of key…
doi.org
September 18, 2025 at 10:01 AM
#SynBio never ceases to amaze: a new study has succeeded in increasing energy metabolism in mammalian cells, algae, and fungi through the expression of E. coli LplA, a lipoylation protein. The result? Read here 👉 doi.org/10.1038/s414...
Why choose between standardisation and adaptability when you can have both? With #SEVA, you can mix and match:
✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment
Build your reproducible #SynBio project here👉 seva-plasmids.com
✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment
Build your reproducible #SynBio project here👉 seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
September 16, 2025 at 10:00 AM
Why choose between standardisation and adaptability when you can have both? With #SEVA, you can mix and match:
✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment
Build your reproducible #SynBio project here👉 seva-plasmids.com
✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment
Build your reproducible #SynBio project here👉 seva-plasmids.com
Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research
RESEARCH | Tom Ellis Lab
Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…
www.tomellislab.com
September 12, 2025 at 9:30 AM
Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research