Ronald Beavis ❌
@ronbeavis.bsky.social
Doing science for money since 1981.
Putting it online since 1995.
Does not engage in cancer research.
QA >> QC
I enjoy doing things I am not very good at doing.
Putting it online since 1995.
Does not engage in cancer research.
QA >> QC
I enjoy doing things I am not very good at doing.
Nuclear receptor subfamily 3 group C member 1 (NR3C1:p) has 2 rigid features: a C-terminal ligand binding (in this case glucocorticoid) domain & a smaller zinc finger domain. The rest is a large dynamic/active region divvied up by a grid of PUZZLE domains (6 pS & 1 pT). #proteomics
December 1, 2025 at 1:01 PM
Nuclear receptor subfamily 3 group C member 1 (NR3C1:p) has 2 rigid features: a C-terminal ligand binding (in this case glucocorticoid) domain & a smaller zinc finger domain. The rest is a large dynamic/active region divvied up by a grid of PUZZLE domains (6 pS & 1 pT). #proteomics
METAPs may require a name change, given the experimental observation of its willingness to remove a leucine when it happens to be at the start of translation. #proteomics
November 30, 2025 at 6:12 PM
METAPs may require a name change, given the experimental observation of its willingness to remove a leucine when it happens to be at the start of translation. #proteomics
The combination of a low pressure system centred in Michigan & a high off-shore of Maine are pumping up some wet air from the Atlantic & the Gulf of the Mad King is generating a storm in the upper Midwestern states & Southern Ontario.
November 30, 2025 at 3:13 PM
The combination of a low pressure system centred in Michigan & a high off-shore of Maine are pumping up some wet air from the Atlantic & the Gulf of the Mad King is generating a storm in the upper Midwestern states & Southern Ontario.
Nuclear receptor interacting protein 1 (NRIP1:p) is believed to interact with the ligand binding domains of nuclear receptors, modulating their transcription factor activity. It does so without any 2°/3° structure, but uses a grid of pS PUZZLE domain controls. #proteomics
November 30, 2025 at 1:18 PM
Nuclear receptor interacting protein 1 (NRIP1:p) is believed to interact with the ligand binding domains of nuclear receptors, modulating their transcription factor activity. It does so without any 2°/3° structure, but uses a grid of pS PUZZLE domain controls. #proteomics
What happens when a cat sits on a laptop keyboard.
November 29, 2025 at 4:38 PM
What happens when a cat sits on a laptop keyboard.
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The shorter one has a homeodomain that isn't in the long one replaced by multiple PUZZLE domains. #proteomics
November 29, 2025 at 12:48 PM
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The shorter one has a homeodomain that isn't in the long one replaced by multiple PUZZLE domains. #proteomics
Ring finger protein 187 (RNF187:p) initiates translation at a "CGT" triplet (Leu), rather than the much more commonly used "AGT" (Met). Interestingly (for me) is the fact that the N-terminal L is co-translationally removed & N-acetyl is added to alanine exposed by that operation. #proteomics
November 28, 2025 at 8:07 PM
Ring finger protein 187 (RNF187:p) initiates translation at a "CGT" triplet (Leu), rather than the much more commonly used "AGT" (Met). Interestingly (for me) is the fact that the N-terminal L is co-translationally removed & N-acetyl is added to alanine exposed by that operation. #proteomics
Is Gemini's review fair or just AI slop?
November 28, 2025 at 2:31 PM
Is Gemini's review fair or just AI slop?
When Gemini is asked: "What is the best brand of mass spectrometer to use in proteomics experiments?", after a lot of slop it answers:
November 28, 2025 at 1:48 PM
When Gemini is asked: "What is the best brand of mass spectrometer to use in proteomics experiments?", after a lot of slop it answers:
The polar vortex is continuing to open up over the North Atlantic, with the counter-rotating system now centred on the northern shore of the Yukon. (winds @ 10 hPa)
November 28, 2025 at 12:53 PM
The polar vortex is continuing to open up over the North Atlantic, with the counter-rotating system now centred on the northern shore of the Yukon. (winds @ 10 hPa)
Like NCOR1:p, nuclear receptor corepressor 2 (NCOR2:p) has ~5% of its sequence tied up in rigid structure (2× homeobox domains of unknown DNA specificity). The remaining 95% of the protein is chopped up into >10 evolutionarily conserved dynamic PUZZLE domains that do the work here. #proteomics
November 28, 2025 at 12:46 PM
Like NCOR1:p, nuclear receptor corepressor 2 (NCOR2:p) has ~5% of its sequence tied up in rigid structure (2× homeobox domains of unknown DNA specificity). The remaining 95% of the protein is chopped up into >10 evolutionarily conserved dynamic PUZZLE domains that do the work here. #proteomics
As predicted, the polar vortex has opened up with a high pressure circulation appearing over Alaska. (winds at 10 hPa)
November 27, 2025 at 2:41 PM
As predicted, the polar vortex has opened up with a high pressure circulation appearing over Alaska. (winds at 10 hPa)
Nuclear receptor corepressor 1 (NCOR1:p) is yet another protein involved in gene expression that is largely dynamic structure, with 2 little (50 aa) homeobox nuggets. The sequence is divvied up into segments by at least 10 pS PUZZLE domains. #proteomics
November 27, 2025 at 1:11 PM
Nuclear receptor corepressor 1 (NCOR1:p) is yet another protein involved in gene expression that is largely dynamic structure, with 2 little (50 aa) homeobox nuggets. The sequence is divvied up into segments by at least 10 pS PUZZLE domains. #proteomics
I finally broke down and bought an inline power meter. This will occupy far too much of my free time for the next month or two.
November 26, 2025 at 3:17 PM
I finally broke down and bought an inline power meter. This will occupy far too much of my free time for the next month or two.
AT-hook containing transcription factor 1 (AH CTF1:p) has 2 interaction domains: a β-propeller & an α helix jumble, with a very narrow pS acceptor domain slotted between the two. Continuing cward, there are multiple pS acceptor PUZZLE domains located at low complexity transitions. #proteomics
November 26, 2025 at 1:01 PM
AT-hook containing transcription factor 1 (AH CTF1:p) has 2 interaction domains: a β-propeller & an α helix jumble, with a very narrow pS acceptor domain slotted between the two. Continuing cward, there are multiple pS acceptor PUZZLE domains located at low complexity transitions. #proteomics
Serine arginine rich matrix protein 1 (SRRM1:p) is another mRNA handling protein that eschews the cold affectation of rigid structures for the warm flexibility & comfort of dynamic ones. It also positively glimmers with gridded pS bedazzled PUZZLE domains. #proteomics
November 25, 2025 at 1:15 PM
Serine arginine rich matrix protein 1 (SRRM1:p) is another mRNA handling protein that eschews the cold affectation of rigid structures for the warm flexibility & comfort of dynamic ones. It also positively glimmers with gridded pS bedazzled PUZZLE domains. #proteomics
Maybe a video would be better.
November 25, 2025 at 1:06 PM
Maybe a video would be better.
The polar vortex this morning is starting to open up a bit: normally it just circles the North Pole. (wind at 10 hPa)
November 25, 2025 at 12:50 PM
The polar vortex this morning is starting to open up a bit: normally it just circles the North Pole. (wind at 10 hPa)
Splicing factor 3B subunit 1 (SF3B1:p) looks as though there is something weird going on: a big, bright cward rigid region (HEAT tandem repeat domains) & a big, black nward dynamic region with one or more PUZZLE domains that look like they were scribbled on by a toddler with a red crayon #proteomics
November 24, 2025 at 1:24 PM
Splicing factor 3B subunit 1 (SF3B1:p) looks as though there is something weird going on: a big, bright cward rigid region (HEAT tandem repeat domains) & a big, black nward dynamic region with one or more PUZZLE domains that look like they were scribbled on by a toddler with a red crayon #proteomics
#Proteomics_101 Based on the post-translational modifications listed on this diagram, provide an argument to determine which cellular compartment each of the 15 soluble regions of this ER membrane protein are exposed to in vivo (TMs in grey). How could you experimentally confirm these assignments?
November 23, 2025 at 10:59 PM
#Proteomics_101 Based on the post-translational modifications listed on this diagram, provide an argument to determine which cellular compartment each of the 15 soluble regions of this ER membrane protein are exposed to in vivo (TMs in grey). How could you experimentally confirm these assignments?
Comparative rates of acquisition achieved via high-throughput biomedical methods. The scale is set by the observation that the number of points of wisdom per individual integrated over a lifetime is 1 ± 1.
November 23, 2025 at 3:46 PM
Comparative rates of acquisition achieved via high-throughput biomedical methods. The scale is set by the observation that the number of points of wisdom per individual integrated over a lifetime is 1 ± 1.
I can't find a verifiable attribution for the source of this one, but it strikes me as an accurate reflection of my attitude towards modern page design.
November 23, 2025 at 1:48 PM
I can't find a verifiable attribution for the source of this one, but it strikes me as an accurate reflection of my attitude towards modern page design.
BCLAF1 And THRAP3 Family Member 3 (BLCAF3:p) has a not very helpful, rather wonky name. It has no observable rigid structure & a handful of major PUZZLE domains laid out in a grid. #proteomics
November 23, 2025 at 12:44 PM
BCLAF1 And THRAP3 Family Member 3 (BLCAF3:p) has a not very helpful, rather wonky name. It has no observable rigid structure & a handful of major PUZZLE domains laid out in a grid. #proteomics
For those who enjoy an electronic engineering analogy, I would propose this to be an appropriate gadget to use when representing this type of protein:
November 22, 2025 at 1:31 PM
For those who enjoy an electronic engineering analogy, I would propose this to be an appropriate gadget to use when representing this type of protein:
Thyroid hormone receptor-associated protein 3 (THRAP3:p) is involved in nuclear transcription activation. It has no observed (or predicted) rigid structure domains, but its dynamic structure has at least 7 evolutionarily conserved, pS acceptor PUZZLE domains. #proteomics
November 22, 2025 at 1:14 PM
Thyroid hormone receptor-associated protein 3 (THRAP3:p) is involved in nuclear transcription activation. It has no observed (or predicted) rigid structure domains, but its dynamic structure has at least 7 evolutionarily conserved, pS acceptor PUZZLE domains. #proteomics