Norman Rzepka
@normanrz.com
Co-Founder of scalable minds. Building WEBKNOSSOS, a tool visualizing, annotation, managing and sharing of large 3D images. Contributing to OME-Zarr.
he/him @[email protected]
he/him @[email protected]
Reposted by Norman Rzepka
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Method of the Year 2025: electron microscopy-based connectomics - Nature Methods
A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...
www.nature.com
December 8, 2025 at 4:59 PM
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Reposted by Norman Rzepka
Connectomics is Method of the Year 2025!!
@natmethods.nature.com
just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...
@natmethods.nature.com
just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...
December 8, 2025 at 4:44 PM
Connectomics is Method of the Year 2025!!
@natmethods.nature.com
just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...
@natmethods.nature.com
just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...
Reposted by Norman Rzepka
Maybe @normanrz.com can provide more @webknossos.org socks to help with the shoe-filling?
December 4, 2025 at 6:17 PM
Maybe @normanrz.com can provide more @webknossos.org socks to help with the shoe-filling?
Reposted by Norman Rzepka
#paper!
X-rays can resolve #ultrastructure in tissues non-destructively.
www.nature.com/articles/s41...
Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.
X-rays can resolve #ultrastructure in tissues non-destructively.
www.nature.com/articles/s41...
Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.
Nondestructive X-ray tomography of brain tissue ultrastructure - Nature Methods
Optimizations of X-ray nanotomography including the choice of resin allows high-resolution imaging of mouse brain tissue, approaching the resolution of volume electron microscopy. Since it does not re...
www.nature.com
November 27, 2025 at 11:51 AM
#paper!
X-rays can resolve #ultrastructure in tissues non-destructively.
www.nature.com/articles/s41...
Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.
X-rays can resolve #ultrastructure in tissues non-destructively.
www.nature.com/articles/s41...
Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.
Reposted by Norman Rzepka
Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...
November 27, 2025 at 10:21 AM
Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...
Reposted by Norman Rzepka
Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41...
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
November 27, 2025 at 4:40 AM
Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41...
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
Reposted by Norman Rzepka
Today I'm announcing a new digital textbook 📖🖥️, "An Introduction to OME-Zarr for Big Bioimaging Data".
ome-zarr-book.readthedocs.io
ome-zarr-book.readthedocs.io
An Introduction to OME-Zarr for Big Bioimaging Data
ome-zarr-book.readthedocs.io
November 4, 2025 at 3:33 PM
Today I'm announcing a new digital textbook 📖🖥️, "An Introduction to OME-Zarr for Big Bioimaging Data".
ome-zarr-book.readthedocs.io
ome-zarr-book.readthedocs.io
Reposted by Norman Rzepka
🌟Enhancements🌟
The proofreading tool got smarter: you can now define multiple segments around a desired split, and it will automatically pick the optimal split point.
The proofreading tool got smarter: you can now define multiple segments around a desired split, and it will automatically pick the optimal split point.
October 9, 2025 at 12:48 PM
🌟Enhancements🌟
The proofreading tool got smarter: you can now define multiple segments around a desired split, and it will automatically pick the optimal split point.
The proofreading tool got smarter: you can now define multiple segments around a desired split, and it will automatically pick the optimal split point.
Reposted by Norman Rzepka
🌟Enhancements🌟
Shared annotations got a big upgrade: they now update live—see your collaborators’ changes as they happen. Simultaneous editing is on the horizon 👀
Shared annotations got a big upgrade: they now update live—see your collaborators’ changes as they happen. Simultaneous editing is on the horizon 👀
September 18, 2025 at 12:48 PM
🌟Enhancements🌟
Shared annotations got a big upgrade: they now update live—see your collaborators’ changes as they happen. Simultaneous editing is on the horizon 👀
Shared annotations got a big upgrade: they now update live—see your collaborators’ changes as they happen. Simultaneous editing is on the horizon 👀
Reposted by Norman Rzepka
#Nanoimaging symposium @crick.ac.uk!
At #CrickXrays25 we'll tackle:
💫synchrotron upgrades
📈current limits in throughput, volume, resolution
🧪sample preparation for X-ray PC imaging
🧠♥️applications in neuroscience and tissue life science
🗓️13th October 2025
🇬🇧🌐 London & online
tinyurl.com/crickxrays25
At #CrickXrays25 we'll tackle:
💫synchrotron upgrades
📈current limits in throughput, volume, resolution
🧪sample preparation for X-ray PC imaging
🧠♥️applications in neuroscience and tissue life science
🗓️13th October 2025
🇬🇧🌐 London & online
tinyurl.com/crickxrays25
Hard X-ray imaging of biological soft tissues symposium 2025
tinyurl.com
September 23, 2025 at 10:43 AM
#Nanoimaging symposium @crick.ac.uk!
At #CrickXrays25 we'll tackle:
💫synchrotron upgrades
📈current limits in throughput, volume, resolution
🧪sample preparation for X-ray PC imaging
🧠♥️applications in neuroscience and tissue life science
🗓️13th October 2025
🇬🇧🌐 London & online
tinyurl.com/crickxrays25
At #CrickXrays25 we'll tackle:
💫synchrotron upgrades
📈current limits in throughput, volume, resolution
🧪sample preparation for X-ray PC imaging
🧠♥️applications in neuroscience and tissue life science
🗓️13th October 2025
🇬🇧🌐 London & online
tinyurl.com/crickxrays25
Reposted by Norman Rzepka
Want to hack with ~40 motivated developers in the OME-Zarr space this fall in Zurich, November 12 - 14th? The application window for the 2025 OME-NGFF workflows hackathon is closing on September 30th! Apply here to be part of this exciting week about OME-Zarr: forms.gle/849cf2fBk1pJ...
September 18, 2025 at 9:42 AM
Want to hack with ~40 motivated developers in the OME-Zarr space this fall in Zurich, November 12 - 14th? The application window for the 2025 OME-NGFF workflows hackathon is closing on September 30th! Apply here to be part of this exciting week about OME-Zarr: forms.gle/849cf2fBk1pJ...
Reposted by Norman Rzepka
🎉 Our paper “MIFA: Metadata, Incentives, Formats and Accessibility guidelines to improve the reuse of AI datasets for bioimage analysis” is out in @natmethods.nature.com!
Community-driven standards to make bioimage data AI-ready & reusable.
👉 www.nature.com/articles/s41... #AI #Bioimaging #FAIRdata
Community-driven standards to make bioimage data AI-ready & reusable.
👉 www.nature.com/articles/s41... #AI #Bioimaging #FAIRdata
September 15, 2025 at 9:57 AM
🎉 Our paper “MIFA: Metadata, Incentives, Formats and Accessibility guidelines to improve the reuse of AI datasets for bioimage analysis” is out in @natmethods.nature.com!
Community-driven standards to make bioimage data AI-ready & reusable.
👉 www.nature.com/articles/s41... #AI #Bioimaging #FAIRdata
Community-driven standards to make bioimage data AI-ready & reusable.
👉 www.nature.com/articles/s41... #AI #Bioimaging #FAIRdata
Reposted by Norman Rzepka
Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧵(1/9)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy - Nature Methods
CLEM-Reg automates the three-dimensional alignment of volume correlative light and electron microscopy datasets by leveraging probabilistic point cloud registration techniques for fast and accurate re...
www.nature.com
September 10, 2025 at 9:37 AM
Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧵(1/9)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Norman Rzepka
#preprint:
We optimised the preparation of tissues for #X-ray phase contrast imaging.
Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.
The process largely preserves #ultrastructure.
@safekhan.bsky.social @andreas-t-schaefer.bsky.social
We optimised the preparation of tissues for #X-ray phase contrast imaging.
Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.
The process largely preserves #ultrastructure.
@safekhan.bsky.social @andreas-t-schaefer.bsky.social
Our pre-print is out!
Critical point drying of brain tissue for x-ray phase contrast imaging.
@apeart.bsky.social @yuxinzhang.bsky.social @jkbrmn.bsky.social @apacureanu.bsky.social @andreas-t-schaefer.bsky.social
@carlesbosch.bsky.social
@esrf.fr
@crick.ac.uk
bioarXiv: doi.org/10.1101/2025...
Critical point drying of brain tissue for x-ray phase contrast imaging.
@apeart.bsky.social @yuxinzhang.bsky.social @jkbrmn.bsky.social @apacureanu.bsky.social @andreas-t-schaefer.bsky.social
@carlesbosch.bsky.social
@esrf.fr
@crick.ac.uk
bioarXiv: doi.org/10.1101/2025...
Critical point drying of brain tissue for X-ray phase contrast imaging
X-ray phase contrast tomography is emerging as a powerful method for imaging large volumes of brain tissue at sub-cellular resolution. However, current sample preparation methods are largely inherited...
doi.org
September 10, 2025 at 3:51 PM
#preprint:
We optimised the preparation of tissues for #X-ray phase contrast imaging.
Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.
The process largely preserves #ultrastructure.
@safekhan.bsky.social @andreas-t-schaefer.bsky.social
We optimised the preparation of tissues for #X-ray phase contrast imaging.
Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.
The process largely preserves #ultrastructure.
@safekhan.bsky.social @andreas-t-schaefer.bsky.social
Reposted by Norman Rzepka
We're happy to announce the 2025 International OME-NGFF workshop 🎉
📅 Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025
📍 Location: Zurich, Switzerland
Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up...
#bioimaging #openscience #OMENGFF #OMEZarr
(1/x 🧵)
📅 Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025
📍 Location: Zurich, Switzerland
Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up...
#bioimaging #openscience #OMENGFF #OMEZarr
(1/x 🧵)
August 25, 2025 at 9:07 AM
We're happy to announce the 2025 International OME-NGFF workshop 🎉
📅 Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025
📍 Location: Zurich, Switzerland
Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up...
#bioimaging #openscience #OMENGFF #OMEZarr
(1/x 🧵)
📅 Dates:
- Symposium: Nov 10–11, 2025
- Hackathon: Nov 12–14, 2025
📍 Location: Zurich, Switzerland
Full details & registration: www.biovisioncenter.uzh.ch/en/events/Up...
#bioimaging #openscience #OMENGFF #OMEZarr
(1/x 🧵)
Reposted by Norman Rzepka
🌟Enhancements🌟
ICYMI: Webknossos viewports are now rotatable.
Use keyboard shortcuts to rotate the orthogonal viewport. Try Shift + Left, Shift + Right, Shift + Up and Shift + Down.
Learn more about keyboard shortcuts: docs.webknossos.org/webknossos/u...
ICYMI: Webknossos viewports are now rotatable.
Use keyboard shortcuts to rotate the orthogonal viewport. Try Shift + Left, Shift + Right, Shift + Up and Shift + Down.
Learn more about keyboard shortcuts: docs.webknossos.org/webknossos/u...
August 14, 2025 at 3:47 PM
🌟Enhancements🌟
ICYMI: Webknossos viewports are now rotatable.
Use keyboard shortcuts to rotate the orthogonal viewport. Try Shift + Left, Shift + Right, Shift + Up and Shift + Down.
Learn more about keyboard shortcuts: docs.webknossos.org/webknossos/u...
ICYMI: Webknossos viewports are now rotatable.
Use keyboard shortcuts to rotate the orthogonal viewport. Try Shift + Left, Shift + Right, Shift + Up and Shift + Down.
Learn more about keyboard shortcuts: docs.webknossos.org/webknossos/u...
Reposted by Norman Rzepka
🌟Big new feature🌟
Rotate the viewports. You can now freely rotate the orthogonal viewports. Trace your objects in the best angle.
Rotate the viewports. You can now freely rotate the orthogonal viewports. Trace your objects in the best angle.
July 31, 2025 at 3:06 PM
🌟Big new feature🌟
Rotate the viewports. You can now freely rotate the orthogonal viewports. Trace your objects in the best angle.
Rotate the viewports. You can now freely rotate the orthogonal viewports. Trace your objects in the best angle.
Reposted by Norman Rzepka
🌟Enhancement🌟
Make the 3D meshes of your segments translucent. Great for revealing inner segments, such as as cell organelles. Also, translucent meshes look really cool 😎
Make the 3D meshes of your segments translucent. Great for revealing inner segments, such as as cell organelles. Also, translucent meshes look really cool 😎
July 17, 2025 at 2:27 PM
🌟Enhancement🌟
Make the 3D meshes of your segments translucent. Great for revealing inner segments, such as as cell organelles. Also, translucent meshes look really cool 😎
Make the 3D meshes of your segments translucent. Great for revealing inner segments, such as as cell organelles. Also, translucent meshes look really cool 😎
Reposted by Norman Rzepka
Hey bioimaging folks, join us for the next OME-NGFF Community Call on June 24 at 9:00 CEST and 17:00 CEST!
Details and agenda on the one and only image.sc here: forum.image.sc/t/join-us-fo...
Details and agenda on the one and only image.sc here: forum.image.sc/t/join-us-fo...
June 3, 2025 at 10:17 AM
Hey bioimaging folks, join us for the next OME-NGFF Community Call on June 24 at 9:00 CEST and 17:00 CEST!
Details and agenda on the one and only image.sc here: forum.image.sc/t/join-us-fo...
Details and agenda on the one and only image.sc here: forum.image.sc/t/join-us-fo...
Reposted by Norman Rzepka
Reposted by Norman Rzepka
May 5, 2025 at 5:54 PM
Reposted by Norman Rzepka
#preprint:
We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.
doi.org/10.1101/2025...
@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr
We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.
doi.org/10.1101/2025...
@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr
May 1, 2025 at 1:39 PM
#preprint:
We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.
doi.org/10.1101/2025...
@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr
We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.
doi.org/10.1101/2025...
@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr
Reposted by Norman Rzepka
My PhD project is out as a preprint!
We combined 2P and synchrotron X-ray to understand mouse olfactory bulb circuits, linking physiology to structure in 3 animals!
doi.org/10.1101/2025...
🙌 @carlesbosch.bsky.social, @apacureanu.bsky.social, @andreas-t-schaefer.bsky.social, @esrf.fr, @crick.ac.uk
We combined 2P and synchrotron X-ray to understand mouse olfactory bulb circuits, linking physiology to structure in 3 animals!
doi.org/10.1101/2025...
🙌 @carlesbosch.bsky.social, @apacureanu.bsky.social, @andreas-t-schaefer.bsky.social, @esrf.fr, @crick.ac.uk
May 1, 2025 at 12:20 PM
My PhD project is out as a preprint!
We combined 2P and synchrotron X-ray to understand mouse olfactory bulb circuits, linking physiology to structure in 3 animals!
doi.org/10.1101/2025...
🙌 @carlesbosch.bsky.social, @apacureanu.bsky.social, @andreas-t-schaefer.bsky.social, @esrf.fr, @crick.ac.uk
We combined 2P and synchrotron X-ray to understand mouse olfactory bulb circuits, linking physiology to structure in 3 animals!
doi.org/10.1101/2025...
🙌 @carlesbosch.bsky.social, @apacureanu.bsky.social, @andreas-t-schaefer.bsky.social, @esrf.fr, @crick.ac.uk
Reposted by Norman Rzepka
Super happy that our papers #2 and #3 from our 3-person lab just went online on bioRxiv! First large-scale 3D EM dataset from mouse hippocampus CA1 www.biorxiv.org/content/10.1... - check out the beautiful data on webknossos wklink.org/7023 ! …
April 23, 2025 at 7:20 AM
Super happy that our papers #2 and #3 from our 3-person lab just went online on bioRxiv! First large-scale 3D EM dataset from mouse hippocampus CA1 www.biorxiv.org/content/10.1... - check out the beautiful data on webknossos wklink.org/7023 ! …
Reposted by Norman Rzepka
If you want to learn the latest developments in volumeEM from the experts, with hands-on experience on our instruments @embl.org, this course is for you! 🔬
🔬 The practical course #EMBOvEM is open for registration 👉 https://s.embl.org/vem25-01-bl
You will gain theoretical and hands-on knowledge on the three main techniques for volume EM – microtome-based serial block-face SEM, focused ion beam SEM, and array tomography.
#vEM #Microscopy
You will gain theoretical and hands-on knowledge on the three main techniques for volume EM – microtome-based serial block-face SEM, focused ion beam SEM, and array tomography.
#vEM #Microscopy
April 24, 2025 at 12:17 PM
If you want to learn the latest developments in volumeEM from the experts, with hands-on experience on our instruments @embl.org, this course is for you! 🔬