Masahiro Ono @ Tocky Lab
monotockylab.bsky.social
Masahiro Ono @ Tocky Lab
@monotockylab.bsky.social
Immunologist | Creator and Developer of Tocky | Integrative Experimental and Computational Immunology | T cells | Cancer Immunology | Immunotherapy
Imperial College London, Reader
Website: monotockylab.github.io
GitHub: github.com/MonoTockyLab
Bored of black-box UMAP and clustering in #Cytometry?

GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.

No dimensionality reduction, just interpretable gates. 🌳
July 13, 2025 at 12:25 PM
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
July 5, 2025 at 8:31 PM
What are self-reactive T cells? Self-reactivity is a vaguely defined concept.
To be quantitative, here is our working model: Self-reactive T cells receive cognate antigen signals in the absence of inflammation or infection, and become Timer⁺ in the Nr4a3-Tocky system(3/4)
rupress.org/jcb/article/...
April 26, 2025 at 12:32 PM
Pleased to announce TockyPrep update - the R package for preprocessing flow cytometric Fluorescent Timer data 🚀
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
January 18, 2025 at 8:14 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 20
December 25, 2024 at 6:57 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 19
December 24, 2024 at 11:38 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 18
December 23, 2024 at 9:12 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 17
December 22, 2024 at 12:34 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 16
December 21, 2024 at 4:53 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 15
December 20, 2024 at 3:50 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 14
December 19, 2024 at 2:54 PM
Indeed, since Part II is even more powerful, combining it with Part I here makes perfect sense.

#BookChallenge
Day 13
December 18, 2024 at 10:36 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 13
December 18, 2024 at 10:17 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 12
December 17, 2024 at 10:37 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 11
December 16, 2024 at 6:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 10
December 15, 2024 at 2:11 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 9
December 14, 2024 at 7:44 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 8
December 13, 2024 at 7:52 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 7
December 12, 2024 at 12:20 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 6
December 10, 2024 at 9:45 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 5
December 9, 2024 at 8:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 4
December 8, 2024 at 11:52 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 3
December 7, 2024 at 8:14 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 2
December 6, 2024 at 10:49 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky💙📚
#BookChallenge

Day 1
December 5, 2024 at 8:52 PM