Masahiro Ono @ Tocky Lab
@monotockylab.bsky.social
Immunologist | Creator and Developer of Tocky | Integrative Experimental and Computational Immunology | T cells | Cancer Immunology | Immunotherapy
Imperial College London, Reader
Website: monotockylab.github.io
GitHub: github.com/MonoTockyLab
Imperial College London, Reader
Website: monotockylab.github.io
GitHub: github.com/MonoTockyLab
Bored of black-box UMAP and clustering in #Cytometry?
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
July 13, 2025 at 12:25 PM
Bored of black-box UMAP and clustering in #Cytometry?
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
GatingTree skips such methods, directly analyses high-dimensional data, builds reproducible tree paths across markers, and reveals group-specific effects.
No dimensionality reduction, just interpretable gates. 🌳
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
July 5, 2025 at 8:31 PM
🚀Thrilled to announce our new Tocky-CRISPR-Deep Learning paper!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
✅ConvNet + Grad-CAM decodes fluorescent timer (Tocky) data
✅Enables single-cell identification of enhancer-dependent Foxp3 transcription dynamics
www.nature.com/articles/s41...
🔥A step toward data-driven immunology!
What are self-reactive T cells? Self-reactivity is a vaguely defined concept.
To be quantitative, here is our working model: Self-reactive T cells receive cognate antigen signals in the absence of inflammation or infection, and become Timer⁺ in the Nr4a3-Tocky system(3/4)
rupress.org/jcb/article/...
To be quantitative, here is our working model: Self-reactive T cells receive cognate antigen signals in the absence of inflammation or infection, and become Timer⁺ in the Nr4a3-Tocky system(3/4)
rupress.org/jcb/article/...
April 26, 2025 at 12:32 PM
What are self-reactive T cells? Self-reactivity is a vaguely defined concept.
To be quantitative, here is our working model: Self-reactive T cells receive cognate antigen signals in the absence of inflammation or infection, and become Timer⁺ in the Nr4a3-Tocky system(3/4)
rupress.org/jcb/article/...
To be quantitative, here is our working model: Self-reactive T cells receive cognate antigen signals in the absence of inflammation or infection, and become Timer⁺ in the Nr4a3-Tocky system(3/4)
rupress.org/jcb/article/...
Pleased to announce TockyPrep update - the R package for preprocessing flow cytometric Fluorescent Timer data 🚀
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
January 18, 2025 at 8:14 AM
Pleased to announce TockyPrep update - the R package for preprocessing flow cytometric Fluorescent Timer data 🚀
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
monotockylab.github.io/TockyPrep/in...
Now featuring an Interactive application to fine-tune your analysis! 🛠️
monotockylab.github.io/TockyPrep/ar...
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 20
#Books
#BookSky💙📚
#BookChallenge
Day 20
December 25, 2024 at 6:57 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 20
#Books
#BookSky💙📚
#BookChallenge
Day 20
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 19
#Books
#BookSky💙📚
#BookChallenge
Day 19
December 24, 2024 at 11:38 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 19
#Books
#BookSky💙📚
#BookChallenge
Day 19
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 18
#Books
#BookSky💙📚
#BookChallenge
Day 18
December 23, 2024 at 9:12 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 18
#Books
#BookSky💙📚
#BookChallenge
Day 18
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 17
#Books
#BookSky💙📚
#BookChallenge
Day 17
December 22, 2024 at 12:34 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 17
#Books
#BookSky💙📚
#BookChallenge
Day 17
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 16
#Books
#BookSky💙📚
#BookChallenge
Day 16
December 21, 2024 at 4:53 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 16
#Books
#BookSky💙📚
#BookChallenge
Day 16
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 15
#Books
#BookSky💙📚
#BookChallenge
Day 15
December 20, 2024 at 3:50 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 15
#Books
#BookSky💙📚
#BookChallenge
Day 15
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 14
#Books
#BookSky💙📚
#BookChallenge
Day 14
December 19, 2024 at 2:54 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 14
#Books
#BookSky💙📚
#BookChallenge
Day 14
Indeed, since Part II is even more powerful, combining it with Part I here makes perfect sense.
#BookChallenge
Day 13
#BookChallenge
Day 13
December 18, 2024 at 10:36 AM
Indeed, since Part II is even more powerful, combining it with Part I here makes perfect sense.
#BookChallenge
Day 13
#BookChallenge
Day 13
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 13
#Books
#BookSky💙📚
#BookChallenge
Day 13
December 18, 2024 at 10:17 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 13
#Books
#BookSky💙📚
#BookChallenge
Day 13
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 12
#Books
#BookSky💙📚
#BookChallenge
Day 12
December 17, 2024 at 10:37 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 12
#Books
#BookSky💙📚
#BookChallenge
Day 12
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 11
#Books
#BookSky💙📚
#BookChallenge
Day 11
December 16, 2024 at 6:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 11
#Books
#BookSky💙📚
#BookChallenge
Day 11
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 10
#Books
#BookSky💙📚
#BookChallenge
Day 10
December 15, 2024 at 2:11 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 10
#Books
#BookSky💙📚
#BookChallenge
Day 10
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 9
#Books
#BookSky💙📚
#BookChallenge
Day 9
December 14, 2024 at 7:44 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 9
#Books
#BookSky💙📚
#BookChallenge
Day 9
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 8
#Books
#BookSky💙📚
#BookChallenge
Day 8
December 13, 2024 at 7:52 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 8
#Books
#BookSky💙📚
#BookChallenge
Day 8
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 7
#Books
#BookSky💙📚
#BookChallenge
Day 7
December 12, 2024 at 12:20 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 7
#Books
#BookSky💙📚
#BookChallenge
Day 7
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 6
#Books
#BookSky💙📚
#BookChallenge
Day 6
December 10, 2024 at 9:45 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 6
#Books
#BookSky💙📚
#BookChallenge
Day 6
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 5
#Books
#BookSky💙📚
#BookChallenge
Day 5
December 9, 2024 at 8:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 5
#Books
#BookSky💙📚
#BookChallenge
Day 5
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 4
#Books
#BookSky💙📚
#BookChallenge
Day 4
December 8, 2024 at 11:52 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 4
#Books
#BookSky💙📚
#BookChallenge
Day 4
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 3
#Books
#BookSky💙📚
#BookChallenge
Day 3
December 7, 2024 at 8:14 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 3
#Books
#BookSky💙📚
#BookChallenge
Day 3
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 2
#Books
#BookSky💙📚
#BookChallenge
Day 2
December 6, 2024 at 10:49 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 2
#Books
#BookSky💙📚
#BookChallenge
Day 2
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 1
#Books
#BookSky💙📚
#BookChallenge
Day 1
December 5, 2024 at 8:52 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
#Books
#BookSky💙📚
#BookChallenge
Day 1
#Books
#BookSky💙📚
#BookChallenge
Day 1