Luke E Holman
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lukeeholman.bsky.social
Luke E Holman
@lukeeholman.bsky.social
PostDoc at the Globe Institute, Copenhagen | #biodiversity | #invasions | #eDNA | #OceanOptimist | 🏔️ 🌊 ☕️ 👨‍💻 📈
Thank you to all the amazing authors who trusted us with their research. Not to mention our wonderful artists Jacek Matysiak (www.instagram.com/big.hippo.art/) and Maddy Dall (www.instagram.com/maddydall/) who brought the issue to life with their beautiful illustrations.
July 10, 2025 at 10:02 AM
📉 Very different richness across methods!📉

Our results showed opposing alpha diversity trends:

• Metabarcoding = lower ASV richness in older sediments

• Metagenomics = lower genus richness in younger sediments

A clear reminder that the method truly matters! 🎯
February 21, 2025 at 8:59 AM
🔍 What taxa overlapped? 🔍

We found that only 3 metazoan genera overlapped between methods:

• Oikopleura (larvacean)
• Gadus (cod)
• Clupea (herring)
(+ 🌿seagrass🌿)

Interestingly we found that only metagenomics provided reliable detections beyond 4k years for all taxa.
February 21, 2025 at 8:59 AM
🔬 What did we do?🔬

We sampled a long (30m+), old (20y+) marine sediment core from the Skagerrak, North Sea, performing 18S(v9) metabarcoding and shotgun-metagenomic sequencing to see what these very different techniques tell us about ancient oceans.
February 21, 2025 at 8:59 AM