You mean when only centromes are lost? Yes, because of the Golgi MTOC. The Rios lab paper actually showed it first, check Fig 7 - centrinone + AKAP450 KO destroys both centrosome and Golgi MTOC, and MTs become disorganized, clearly different from WT. www.embopress.org/doi/full/10....

This nice paper by Anna Akhmanova’s group does not address anchoring specifically, but organizing function. The problem is that if you remove centrioles, PCM still clusters or the Golgi takes over. If you prevent that (centrinone + AKAP450 KO) MT are completely disorganized. doi.org/10.7554/eLif...

Not saying motors, plus end regulators, MAPs don’t contribute. I believe some microtubules are anchored, but others are perhaps released - shown for the centrosome. Could be pulling by motors or loss of anchoring/nucleation without anchoring. Quite a knowledge gap here, I would say.

It does not only nucleate, it also anchors minus ends. This determines microtubule orientation (all minus ends at MTOC, all plus ends away from it). Because of this, modulating the shape and distribution of the MTOCs, also controls the overall shape of the network.

Doesn’t nucleation + anchoring of minus ends control microtubule orientation and, through shape and distribution of the MTOC, overall microtubule network shape and polarity? I agree it isn’t always the “center”, but I still think it has organizing activity.

What about their function in anchoring minus ends?

Congrats @araujolabub.bsky.social, make the flies breathe!

I see 6 fold symmetry and 9 fold asymmetry

Harder to focus on the science if it is poorly presented, I’d say.

"Super level" funding programs IMO are a huge waste of money - they benefit big labs at the cost of defunding others. We had big "Foundation grants" in Canada (albeit at a much smaller scale than similar programs in the USA/EU)... TL;DR it nearly ruined the sustainability of our research ecosystem

…as wobbly tentacles that capture you?

Great news and congrats Monica, happy to have you in Barcelona!