Kyle Card
@kylejcard.bsky.social
HHMI Hanna Gray Fellow at the Cleveland Clinic studying drug resistance evolution w/ Jacob Scott 💊 | HHMI Gilliam Fellow & Ph.D. w/ Richard Lenski | #DisabledInSTEM 🦾 | He/Him
I don't understand why groups design plasmids, publish protocol papers centered on their use, and then not deposit them to Addgene.
Open, reproducible science should be our North Star, not an afterthought (or worse, never considered at all).
Open, reproducible science should be our North Star, not an afterthought (or worse, never considered at all).
October 22, 2025 at 2:06 PM
I don't understand why groups design plasmids, publish protocol papers centered on their use, and then not deposit them to Addgene.
Open, reproducible science should be our North Star, not an afterthought (or worse, never considered at all).
Open, reproducible science should be our North Star, not an afterthought (or worse, never considered at all).
Thrilled to share our paper that just came out in PNAS!
We asked a deceptively simple question: What happens when S. aureus adapts to vancomycin, a critical first-line antibiotic?
1/5
We asked a deceptively simple question: What happens when S. aureus adapts to vancomycin, a critical first-line antibiotic?
1/5
September 27, 2025 at 5:32 PM
Thrilled to share our paper that just came out in PNAS!
We asked a deceptively simple question: What happens when S. aureus adapts to vancomycin, a critical first-line antibiotic?
1/5
We asked a deceptively simple question: What happens when S. aureus adapts to vancomycin, a critical first-line antibiotic?
1/5
Reposted by Kyle Card
🚨 Microbiologists! We are recruiting Assistant / Associate Professors in 3 collaborative areas of our U. Pittsburgh School of Medicine.
1) MMG (my dept): fundamental research in med micro
2) Peds ID / I4Kids institute
3) Center for Vaccine Research
🔗 to all 3 w/info: www.linkedin.com/posts/vaughn...
1) MMG (my dept): fundamental research in med micro
2) Peds ID / I4Kids institute
3) Center for Vaccine Research
🔗 to all 3 w/info: www.linkedin.com/posts/vaughn...
Faculty Professor Associate - Full-Time | Vaughn Cooper
We are recruiting Faculty microbiologists in three (3) different, complementary, and collaborative areas at the University of Pittsburgh associated with the School of Medicine.
1) Fundamental researc...
www.linkedin.com
September 23, 2025 at 10:31 PM
🚨 Microbiologists! We are recruiting Assistant / Associate Professors in 3 collaborative areas of our U. Pittsburgh School of Medicine.
1) MMG (my dept): fundamental research in med micro
2) Peds ID / I4Kids institute
3) Center for Vaccine Research
🔗 to all 3 w/info: www.linkedin.com/posts/vaughn...
1) MMG (my dept): fundamental research in med micro
2) Peds ID / I4Kids institute
3) Center for Vaccine Research
🔗 to all 3 w/info: www.linkedin.com/posts/vaughn...
Reposted by Kyle Card
Sharing the most significant work from my group, led by the @evolvingstem.bsky.social team.
Come for the discoveries of how Pseudomonas adapts in biofilms, stay for the story of how they were discovered by thousands of young scientists in grades 9-12. 🧪🧫🧬🧵
www.biorxiv.org/content/10.1...
Come for the discoveries of how Pseudomonas adapts in biofilms, stay for the story of how they were discovered by thousands of young scientists in grades 9-12. 🧪🧫🧬🧵
www.biorxiv.org/content/10.1...
Student-led experimental evolution reveals novel biofilm regulatory networks underlying adaptations to multiple niches
We established a research-education partnership known as EvolvingSTEM that provides secondary school students the opportunity to conduct authentic research experiments centered on microbial evolution....
www.biorxiv.org
June 8, 2025 at 8:09 PM
Sharing the most significant work from my group, led by the @evolvingstem.bsky.social team.
Come for the discoveries of how Pseudomonas adapts in biofilms, stay for the story of how they were discovered by thousands of young scientists in grades 9-12. 🧪🧫🧬🧵
www.biorxiv.org/content/10.1...
Come for the discoveries of how Pseudomonas adapts in biofilms, stay for the story of how they were discovered by thousands of young scientists in grades 9-12. 🧪🧫🧬🧵
www.biorxiv.org/content/10.1...
Reposted by Kyle Card
Another day of reminding everyone of Carl Sagan’s eerily accurate warning about the dangers of not being able to ask skeptical scientific questions to those in power or authority.
May 8, 2025 at 1:14 PM
Another day of reminding everyone of Carl Sagan’s eerily accurate warning about the dangers of not being able to ask skeptical scientific questions to those in power or authority.
Reposted by Kyle Card
You and millions of other people rely on the NPR Network for trustworthy journalism.
NPR’s editorial integrity and independence are non-negotiable. It’s a promise we make to you – a promise we will fight to keep. Now, we need your support.
Donate today: n.pr/3EYClNR
NPR’s editorial integrity and independence are non-negotiable. It’s a promise we make to you – a promise we will fight to keep. Now, we need your support.
Donate today: n.pr/3EYClNR
May 5, 2025 at 8:14 PM
You and millions of other people rely on the NPR Network for trustworthy journalism.
NPR’s editorial integrity and independence are non-negotiable. It’s a promise we make to you – a promise we will fight to keep. Now, we need your support.
Donate today: n.pr/3EYClNR
NPR’s editorial integrity and independence are non-negotiable. It’s a promise we make to you – a promise we will fight to keep. Now, we need your support.
Donate today: n.pr/3EYClNR
Reposted by Kyle Card
NIH's work saves lives—& Trump is gutting it.
Straight from D.C. I headed to Seattle Children's to hear from researchers & patients alike about the importance of NIH funding—it's not just about lines in a budget, it's about lifesaving discoveries.
We ALL need to speak up to save it.
Straight from D.C. I headed to Seattle Children's to hear from researchers & patients alike about the importance of NIH funding—it's not just about lines in a budget, it's about lifesaving discoveries.
We ALL need to speak up to save it.
May 2, 2025 at 9:04 PM
NIH's work saves lives—& Trump is gutting it.
Straight from D.C. I headed to Seattle Children's to hear from researchers & patients alike about the importance of NIH funding—it's not just about lines in a budget, it's about lifesaving discoveries.
We ALL need to speak up to save it.
Straight from D.C. I headed to Seattle Children's to hear from researchers & patients alike about the importance of NIH funding—it's not just about lines in a budget, it's about lifesaving discoveries.
We ALL need to speak up to save it.
Reposted by Kyle Card
Bezos plans to sell ~$4.8 billion of Amazon stock. That's only 2.5% of his holdings in the company.
That sale roughly equals the proposed ~50% cut to NSF.
IOW, with modest endowment-level growth of Amazon, Bezos could fund the entire NSF shortfall into perpetuity.
www.cnbc.com/2025/05/02/j...
That sale roughly equals the proposed ~50% cut to NSF.
IOW, with modest endowment-level growth of Amazon, Bezos could fund the entire NSF shortfall into perpetuity.
www.cnbc.com/2025/05/02/j...
May 2, 2025 at 8:49 PM
Bezos plans to sell ~$4.8 billion of Amazon stock. That's only 2.5% of his holdings in the company.
That sale roughly equals the proposed ~50% cut to NSF.
IOW, with modest endowment-level growth of Amazon, Bezos could fund the entire NSF shortfall into perpetuity.
www.cnbc.com/2025/05/02/j...
That sale roughly equals the proposed ~50% cut to NSF.
IOW, with modest endowment-level growth of Amazon, Bezos could fund the entire NSF shortfall into perpetuity.
www.cnbc.com/2025/05/02/j...
Reposted by Kyle Card
Given today's onslaught on HHS, I'm resharing my OpEd in @elife.bsky.social.
You are not alone, and you do have an important voice!
Many colleagues remain unaware that the foundation of US biomedical research is crumbling under attack. Tell them!
Full text 🔗 elifesciences.org/articles/106...
You are not alone, and you do have an important voice!
Many colleagues remain unaware that the foundation of US biomedical research is crumbling under attack. Tell them!
Full text 🔗 elifesciences.org/articles/106...
April 1, 2025 at 5:09 PM
Given today's onslaught on HHS, I'm resharing my OpEd in @elife.bsky.social.
You are not alone, and you do have an important voice!
Many colleagues remain unaware that the foundation of US biomedical research is crumbling under attack. Tell them!
Full text 🔗 elifesciences.org/articles/106...
You are not alone, and you do have an important voice!
Many colleagues remain unaware that the foundation of US biomedical research is crumbling under attack. Tell them!
Full text 🔗 elifesciences.org/articles/106...
Reposted by Kyle Card
About fifty years ago, NASA strapped a message in a bottle to a rocket and flung it into the deep dark
It wasn’t supposed to go this far, but it did. Long past its mission, it’s still out there so far away now that a simple hello takes a day to reach it, and another day to hear if it says hello back
It wasn’t supposed to go this far, but it did. Long past its mission, it’s still out there so far away now that a simple hello takes a day to reach it, and another day to hear if it says hello back
March 12, 2025 at 5:04 AM
About fifty years ago, NASA strapped a message in a bottle to a rocket and flung it into the deep dark
It wasn’t supposed to go this far, but it did. Long past its mission, it’s still out there so far away now that a simple hello takes a day to reach it, and another day to hear if it says hello back
It wasn’t supposed to go this far, but it did. Long past its mission, it’s still out there so far away now that a simple hello takes a day to reach it, and another day to hear if it says hello back
We're standing up for science in Columbus, OH
March 7, 2025 at 8:13 PM
We're standing up for science in Columbus, OH
Reposted by Kyle Card
TODAY IS THE DAY! 🧪☀️⬇️
Join us at the Lincoln Memorial or your local site to Stand Up for Science!
Join us at the Lincoln Memorial or your local site to Stand Up for Science!
March 7, 2025 at 11:44 AM
TODAY IS THE DAY! 🧪☀️⬇️
Join us at the Lincoln Memorial or your local site to Stand Up for Science!
Join us at the Lincoln Memorial or your local site to Stand Up for Science!
Reposted by Kyle Card
Happy 37th birthday to the LTEE!
the-ltee.org/history/
the-ltee.org/history/
History – The Long-Term Evolution Experiment
the-ltee.org
February 24, 2025 at 3:39 PM
Happy 37th birthday to the LTEE!
the-ltee.org/history/
the-ltee.org/history/
Reposted by Kyle Card
Happy 37th birthday to @relenski.bsky.social long term evolution experiment. Here's a beautiful summary: the-ltee.org/history/
History – The Long-Term Evolution Experiment
the-ltee.org
February 24, 2025 at 6:13 PM
Happy 37th birthday to @relenski.bsky.social long term evolution experiment. Here's a beautiful summary: the-ltee.org/history/
How my Monday is going so far: I wanted to speed up permutation test code. So, I parallelized it and looped it 10,000 times but forgot that the original code was already being looped. What should've been 30,000 total permutations turned into (30,000)^2 = 900 million.
Classic.
Classic.
a cartoon cat is standing in a hallway looking at something .
ALT: a cartoon cat is standing in a hallway looking at something .
media.tenor.com
February 17, 2025 at 4:19 PM
How my Monday is going so far: I wanted to speed up permutation test code. So, I parallelized it and looped it 10,000 times but forgot that the original code was already being looped. What should've been 30,000 total permutations turned into (30,000)^2 = 900 million.
Classic.
Classic.
Reposted by Kyle Card
Under fascism, no one is safe.
Universities stayed quiet as the admin targeted DEI & foreign aid, censored research, etc.
Now, they face devastating cuts to indirect cost rates, threatening research & operations.
Silence won’t protect institutions. It won’t protect people; it never does.
Universities stayed quiet as the admin targeted DEI & foreign aid, censored research, etc.
Now, they face devastating cuts to indirect cost rates, threatening research & operations.
Silence won’t protect institutions. It won’t protect people; it never does.
February 8, 2025 at 1:28 PM
Under fascism, no one is safe.
Universities stayed quiet as the admin targeted DEI & foreign aid, censored research, etc.
Now, they face devastating cuts to indirect cost rates, threatening research & operations.
Silence won’t protect institutions. It won’t protect people; it never does.
Universities stayed quiet as the admin targeted DEI & foreign aid, censored research, etc.
Now, they face devastating cuts to indirect cost rates, threatening research & operations.
Silence won’t protect institutions. It won’t protect people; it never does.
Reposted by Kyle Card
I’ve been seething and grieving since yesterday’s Friday Night Massacre of NIH overheads, a seeming bit of bureaucratic trivial that will in fact destroy the US university system if unchecked. But I want to get away from budgets and rate breakdowns and F&A percentages for a moment.
Humor me?
Humor me?
February 8, 2025 at 9:41 PM
I’ve been seething and grieving since yesterday’s Friday Night Massacre of NIH overheads, a seeming bit of bureaucratic trivial that will in fact destroy the US university system if unchecked. But I want to get away from budgets and rate breakdowns and F&A percentages for a moment.
Humor me?
Humor me?
Reposted by Kyle Card
Another assault on US competitiveness at a time when biomedicine is roaring with innovation, an own-goal in a high-stakes international tournament. Of course the real losers are American people needing medicines and cures.
www.washingtonpost.com/health/2025/...
www.washingtonpost.com/health/2025/...
NIH cuts billions of dollars in biomedical funding, effective immediately
The move halts a large slice of money for most universities and research institutions virtually overnight, imperiling vital research in everything from cancer to heart disease.
www.washingtonpost.com
February 8, 2025 at 5:12 PM
Another assault on US competitiveness at a time when biomedicine is roaring with innovation, an own-goal in a high-stakes international tournament. Of course the real losers are American people needing medicines and cures.
www.washingtonpost.com/health/2025/...
www.washingtonpost.com/health/2025/...
Reposted by Kyle Card
Many of us, and especially early-career scientists, are facing huge challenges now.
However, one of the joys of science is to delve into thinking about research. In that spirit, I offer this blog post on starting a microbial evolution experiment.
telliamedrevisited.wordpress.com/2025/02/08/h...
However, one of the joys of science is to delve into thinking about research. In that spirit, I offer this blog post on starting a microbial evolution experiment.
telliamedrevisited.wordpress.com/2025/02/08/h...
February 8, 2025 at 6:16 PM
Many of us, and especially early-career scientists, are facing huge challenges now.
However, one of the joys of science is to delve into thinking about research. In that spirit, I offer this blog post on starting a microbial evolution experiment.
telliamedrevisited.wordpress.com/2025/02/08/h...
However, one of the joys of science is to delve into thinking about research. In that spirit, I offer this blog post on starting a microbial evolution experiment.
telliamedrevisited.wordpress.com/2025/02/08/h...
Reposted by Kyle Card
Interpretation for the non-scientists: When faculty get NIH grants, the University gets some extra cash to make sure the research is supported. NIH just cut that $$ by a LOT. That means Unis are less likely to support research. Most research in the US is done at Unis, so... it's bad 🧪
February 7, 2025 at 11:43 PM
Interpretation for the non-scientists: When faculty get NIH grants, the University gets some extra cash to make sure the research is supported. NIH just cut that $$ by a LOT. That means Unis are less likely to support research. Most research in the US is done at Unis, so... it's bad 🧪
Reposted by Kyle Card
🚨BREAKING. From a program officer at the National Science Foundation, a list of keywords that can cause a grant to be pulled. I will be sharing screenshots of these keywords along with a decision tree. Please share widely. This is a crisis for academic freedom & science.
February 4, 2025 at 1:26 AM
🚨BREAKING. From a program officer at the National Science Foundation, a list of keywords that can cause a grant to be pulled. I will be sharing screenshots of these keywords along with a decision tree. Please share widely. This is a crisis for academic freedom & science.
Reposted by Kyle Card
"The President’s remarks today suggesting the deadly crash of Flight 5342 may have been the fault of public servants with disabilities are irresponsible, disparaging, and wrong."
www.aapd.com/disability-o...
www.aapd.com/disability-o...
Leading Disability Organizations: President Trump’s Blame of Deadly Plane Crash on Disabled Federal Workers Is Baseless, Irresponsible - AAPD
For Immediate Release: January 30, 2025 Contact: Jess Davidson, [email protected]; 202-465-5528 WASHINGTON D.C. – In remarks today at the White House, President Trump cast potential blame for the tra...
www.aapd.com
January 31, 2025 at 2:46 AM
"The President’s remarks today suggesting the deadly crash of Flight 5342 may have been the fault of public servants with disabilities are irresponsible, disparaging, and wrong."
www.aapd.com/disability-o...
www.aapd.com/disability-o...
Reposted by Kyle Card
I am confident that scientific societies will play an unusually important role in the coming years. If you are a microbiologist of any kind, please consider joining @asm.org if you haven't already. There are many ways to get involved, including advocacy.
To learn more:
asm.org/About-ASM/Vo...
To learn more:
asm.org/About-ASM/Vo...
Volunteer With ASM | Overview
ASM volunteer opportunities range from speaking with legislators about funding research during ASM's annual Hill Day, to writing articles for asm.org, to serving on committees and the Board of Directo...
asm.org
January 23, 2025 at 9:21 PM
I am confident that scientific societies will play an unusually important role in the coming years. If you are a microbiologist of any kind, please consider joining @asm.org if you haven't already. There are many ways to get involved, including advocacy.
To learn more:
asm.org/About-ASM/Vo...
To learn more:
asm.org/About-ASM/Vo...
Reposted by Kyle Card
American science and medicine has been thrown into chaos and uncertainty over the past week. Here are some stories to get up to speed. 1/12
January 28, 2025 at 3:58 AM
American science and medicine has been thrown into chaos and uncertainty over the past week. Here are some stories to get up to speed. 1/12