Incredibly thankful to everyone involved in this project, especially Pawel, my co-conspirator and chloroplast king, and Prof Alison Smith, for immense opportunity, guidance and trust.

It really takes a village to publish a paper sometimes!
@kgeisler.bsky.social @camplantsci.bsky.social

Finally, we use these systems to control the expression of metabolic enzymes in the pathway towards casbene - an industrially relevant diterpenoid - thus modulating casbene production.

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Rather than a bimodal -/+ or on/off switch, we wanted to modulate gene expression across a range of vitamin concentrations. We show robust fine-tuned control of several chloroplast transgenes under these systems.

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