Joost Snijder
@joostsnijder.bsky.social
a sort of scientist. viruses and antibodies. structure and biophysics. mass spec and cryoEM | Voorzitter SP Culemborg
On my way to the @denvms.bsky.social fall meeting to present our antibody sequencing work 😁
November 19, 2025 at 8:39 AM
On my way to the @denvms.bsky.social fall meeting to present our antibody sequencing work 😁
We’re hosting the MS+M conference in Utrecht today, lots of great talks ahead at the intersection of mass spec and microscopy. Here’s @hecklab.bsky.social kicking things off.
October 1, 2025 at 7:38 AM
We’re hosting the MS+M conference in Utrecht today, lots of great talks ahead at the intersection of mass spec and microscopy. Here’s @hecklab.bsky.social kicking things off.
Exciting day. We’re rebooting our advanced proteomics course. Full house, Kelly about to kick things off!
September 22, 2025 at 7:37 AM
Exciting day. We’re rebooting our advanced proteomics course. Full house, Kelly about to kick things off!
Great annual tradition to start off the academic year: The Vaccine Symposium at UMCU. Very interesting talk by Galit Alter on systems serology.
September 11, 2025 at 10:02 AM
Great annual tradition to start off the academic year: The Vaccine Symposium at UMCU. Very interesting talk by Galit Alter on systems serology.
Got to see DAK in Paradiso yesterday. Awesome show
July 29, 2025 at 9:54 AM
Got to see DAK in Paradiso yesterday. Awesome show
We sequenced the anti-RSV-F antibody 131-2a by mass spec and determined its epitope by cryoEM. New insights in postfusion specific binding and antigenic site I. Now published in ACS Infectious Diseases @pubs.acs.org.
pubs.acs.org/doi/10.1021/...
@xdh.bsky.social @hangryhobbit.bsky.social
pubs.acs.org/doi/10.1021/...
@xdh.bsky.social @hangryhobbit.bsky.social
July 22, 2025 at 11:33 AM
We sequenced the anti-RSV-F antibody 131-2a by mass spec and determined its epitope by cryoEM. New insights in postfusion specific binding and antigenic site I. Now published in ACS Infectious Diseases @pubs.acs.org.
pubs.acs.org/doi/10.1021/...
@xdh.bsky.social @hangryhobbit.bsky.social
pubs.acs.org/doi/10.1021/...
@xdh.bsky.social @hangryhobbit.bsky.social
Latest picture of the group, very proud of this bunch 🤩
June 30, 2025 at 12:29 PM
Latest picture of the group, very proud of this bunch 🤩
The improved (depth of) coverage improves sequencing accuracy and potentially saves a lot of instrument time, because so far we’ve always had to run multiple parallel protease digestions.
June 2, 2025 at 7:33 AM
The improved (depth of) coverage improves sequencing accuracy and potentially saves a lot of instrument time, because so far we’ve always had to run multiple parallel protease digestions.
This yields way more unique peptides than trypsin or even chymotrypsin. And the peptides are also often longer, which is great for assembly in de novo sequencing of antibodies.
June 2, 2025 at 7:33 AM
This yields way more unique peptides than trypsin or even chymotrypsin. And the peptides are also often longer, which is great for assembly in de novo sequencing of antibodies.
These new archaeal proteases are great for de novo antibody sequencing by mass spec. Great work by @tshamorkina.bsky.social , Laura and @tkadava.bsky.social together with @hecklab.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
June 2, 2025 at 7:33 AM
These new archaeal proteases are great for de novo antibody sequencing by mass spec. Great work by @tshamorkina.bsky.social , Laura and @tkadava.bsky.social together with @hecklab.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Go find Tatiana Shamorkina, Douwe Schulte, and Weiwei Peng at ASMS this year. They've been very busy with their antibody sequencing work and would love to chat! @tshamorkina.bsky.social
June 1, 2025 at 7:41 AM
Go find Tatiana Shamorkina, Douwe Schulte, and Weiwei Peng at ASMS this year. They've been very busy with their antibody sequencing work and would love to chat! @tshamorkina.bsky.social
Had deze al jaren ongelezen in de kast liggen, maar ben blij dat ik er nu pas aan toe kwam. De wereld is met datasturing en kunstmatige intelligentie steeds meer op die klont uit het boek gaan lijken.
May 28, 2025 at 8:51 PM
Had deze al jaren ongelezen in de kast liggen, maar ben blij dat ik er nu pas aan toe kwam. De wereld is met datasturing en kunstmatige intelligentie steeds meer op die klont uit het boek gaan lijken.
Fun tradition in the BioMS group: our annual international lunch. Big potluck where everyone brings a dish from their home country. I’m very full.
May 27, 2025 at 1:09 PM
Fun tradition in the BioMS group: our annual international lunch. Big potluck where everyone brings a dish from their home country. I’m very full.
Here’s Tineke in action. And out with us for a nice lab dinner. Thanks for a great seminar!
May 26, 2025 at 8:45 PM
Here’s Tineke in action. And out with us for a nice lab dinner. Thanks for a great seminar!
Lots of the O-glycans are present in N-linked NXS/T sequons. We first noticed this in EBOV GP and since paying attention we see this again and again now also in these coronavirus spikes. Wonder what kind of crosstalk is going on here 🤔
pubs.acs.org/doi/10.1021/...
www.nature.com/articles/s42...
pubs.acs.org/doi/10.1021/...
www.nature.com/articles/s42...
May 23, 2025 at 7:12 PM
Lots of the O-glycans are present in N-linked NXS/T sequons. We first noticed this in EBOV GP and since paying attention we see this again and again now also in these coronavirus spikes. Wonder what kind of crosstalk is going on here 🤔
pubs.acs.org/doi/10.1021/...
www.nature.com/articles/s42...
pubs.acs.org/doi/10.1021/...
www.nature.com/articles/s42...
We also find spurious O-glycosylation throughout the spike, with a more dense hotspot in a mucin-like region.
May 23, 2025 at 7:12 PM
We also find spurious O-glycosylation throughout the spike, with a more dense hotspot in a mucin-like region.
We mapped N- and O-linked glycosylation in these unusual spikes by mass spec. Paints a picture of the glycan shield of this important antigen.
May 23, 2025 at 7:12 PM
We mapped N- and O-linked glycosylation in these unusual spikes by mass spec. Paints a picture of the glycan shield of this important antigen.
First time working on dolphin/whale viruses 🐬🦠🐳. Check out our new preprint with @danielhurdiss.bsky.social et al. describing the structure and glycan shield of two cetacean coronavirus spikes. Some neat glycoproteomics work by @tshamorkina.bsky.social in this one.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
May 23, 2025 at 7:12 PM
First time working on dolphin/whale viruses 🐬🦠🐳. Check out our new preprint with @danielhurdiss.bsky.social et al. describing the structure and glycan shield of two cetacean coronavirus spikes. Some neat glycoproteomics work by @tshamorkina.bsky.social in this one.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Ben nu hier in begonnen maar heb er eigenlijk de energie niet voor
May 13, 2025 at 8:08 PM
Ben nu hier in begonnen maar heb er eigenlijk de energie niet voor
Zo hard bouwde je nog niet eerder modderige zandkastelen tegen de vloedlijn aan.
May 13, 2025 at 8:07 PM
Zo hard bouwde je nog niet eerder modderige zandkastelen tegen de vloedlijn aan.
Looking forward to host Tineke Cantaert for a seminar on May 26th. Come check it out if you're interested in dengue, B cells and antibodies.
May 13, 2025 at 8:23 AM
Looking forward to host Tineke Cantaert for a seminar on May 26th. Come check it out if you're interested in dengue, B cells and antibodies.
Bijvoet Symposium today. Zin in! @bijvoet-centre.bsky.social
May 12, 2025 at 7:32 AM
Bijvoet Symposium today. Zin in! @bijvoet-centre.bsky.social
Excited to announce our revamped PhD-level BioMS course this September:
Advanced Proteomics for Drug Discovery and Biotherapeutics
Great line-up of instructors and topics, registration is open:
www.hecklab.com/advanced-pro...
Advanced Proteomics for Drug Discovery and Biotherapeutics
Great line-up of instructors and topics, registration is open:
www.hecklab.com/advanced-pro...
April 29, 2025 at 9:32 AM
Excited to announce our revamped PhD-level BioMS course this September:
Advanced Proteomics for Drug Discovery and Biotherapeutics
Great line-up of instructors and topics, registration is open:
www.hecklab.com/advanced-pro...
Advanced Proteomics for Drug Discovery and Biotherapeutics
Great line-up of instructors and topics, registration is open:
www.hecklab.com/advanced-pro...
Simultaneous polyclonal antibody sequencing and epitope mapping by cryoEM and mass spec: how far can we go? Check out the final version of our paper in eLife:
elifesciences.org/articles/101...
elifesciences.org/articles/101...
April 29, 2025 at 9:18 AM
Simultaneous polyclonal antibody sequencing and epitope mapping by cryoEM and mass spec: how far can we go? Check out the final version of our paper in eLife:
elifesciences.org/articles/101...
elifesciences.org/articles/101...
Tune in Wednesday for my webinar on antiviral antibody sequencing and epitope mapping by mass spec and EM.
www.labroots.com/ms/webinar/s...
www.labroots.com/ms/webinar/s...
April 7, 2025 at 11:45 AM
Tune in Wednesday for my webinar on antiviral antibody sequencing and epitope mapping by mass spec and EM.
www.labroots.com/ms/webinar/s...
www.labroots.com/ms/webinar/s...