Well, since you've read this far, we're already quite familiar with each other. So I'm ready to introduce you not only to spheroids, but also to sausageoids and 3d_cell_culture of ... other shapes.

The work turned out to be as open source as possible, all models were made in
@freecadnews.bsky.social, microscope images were processed in napari, ImageJ and CellProfiler

7/n

If you have a logical question: does the shape approximated by voxels produced by printers interfere with the cells? No, it does not interfere. I was also surprised, but the spheroids form perfectly.

Blue in the video is the CAD model, red is in the slicer, green is what actually comes out.

6/n

I tried to make everything as accessible as possible and specifically checked the reproducibility by distributing 3D models and printed samples of stamps to biologists I know. Everyone succeeded.

Models on GitHub can also be modified to suit specific tasks.

5/n

The required feature size for manufacturing spheroids is in the order of hundreds of micrometres, which is quite realistic for an MSLA printer. I mainly used an old Anycubic Mono, but other printers work just as well.

4/n

The MSLASpheroidStamp project includes 3D printed stamps that can be used to make indentations in agarose for #cell_culture #spheroids directly, and silicone molds (similar to MicroTissue).

3/n

You will need an MSLA 3D printer (around 200–400 USD), molding silicone, and a corner in the laboratory that will get dirty.

The article was published in Bioprinting: tinyurl.com/yjvuj64u
All protocols and models are available on GitHub:
tinyurl.com/3tm69k75

2/n

So, I'm on holiday, so it's the perfect time to post a thread that's been sitting around for a while.

How difficult is it to make your own spheroid production system comparable to the expensive MicroTissue or AggreWell systems?

Easy, actually.

1/n

#DIY #biology #science #3d_printing

Was flagged on pubpeer, but I think for nothing! Great article and a brilliant scientific breakthrough!

www.sciencedirect.com/science/arti...

Let the first post here be a teaser for a paper I'm going to submit to a journal pretty soon. Redundant #DIY, #MSLA 3d printing and #3d_cell_cultures