Find a description in the previous post about the preprint: bsky.app/profile/fzol...

And it's a cover! bsky.app/profile/ibro...

Colegas latinoamericanos: hoy cierra esta excelente oportunidad de reunirse en nuevas redes o sociedades de neurociencias donde no existen, y además, asociarse a IBRO! Último día!

I just realized Santiago is in Bluesky! You can contact him here: @sabr-bio.bsky.social

I personally thank Santi's thesis co-supervisor, Julio A. Hernández. This work was possible thanks to our great graduate program PEDECIBA; to Universidad de la República, which funded research through CSIC and SB fellowship through CAP; to the Advanced Bioimaging Unit at Institut Pasteur Montevideo.

A reduction in apical/basal tension, on the other hand, would produce extensive cell extrusion after a period of relative stability. When extrapolated to a living tissue, these observations could be explained by cell-cell and cell-matrix adhesion, relying on F-actin-generated tension across cells.

Making a long story short, and skipping the quantitative part (if interested, ask Santi), what we found is that the relatively crowded simulated pseudostratified epithelium was stable as long as apical/basal tension was kept high relative to lateral tension. An example is reproduced in the video.

Here is where theoretical modelling enters the story. The goal was to simulate a pseudostratified epithelium using an energy-based 2D vertex model to get cells with flexible borders, able to respond to the changing positions of non-deformable nuclei. The "fake neuroepithelium" is seen from a side.

Apical cell extrusion could be due either to an induction of cell death, folowed by expulsion, or by live cell extrusion, a response to epithelial overcrowding. The experiments in the present report indicate the latter to be responsible for PMA effect, hence: mechanical forces must be involved!

In @gaparicio.bsky.social previous work, we showed that knock-down of the actin-modulating protein MARCKS, or its phosphorylation (by activating PKC with PMA), caused neural tube closure failure, and loss of apico-basal polarity with apical cell extrusion in the chick embryo. doi.org/10.1002/dvg....

A note to Uruguayans: you can have access to all the articles in the Special Issue from Timbó, timbo.org.uy

IBRO gathers more than 90 neuroscience societies all around the globe, supporting with grants the efforts of researchers and students who push the boundaries of knowledge in this relevant area. These funds largely come from these two journals. Publish in @ibrojournals.bsky.social!!

We are also thankful to the whole team at Neuroscience @ibrojournals.bsky.social @elsevierconnect.bsky.social, whose professionalism made this VSI a reality. Neuroscience and Neuroscience Reports are published by the International Brain Research Organization @ibroorg.bsky.social. What is IBRO?

The guest editors, A. Silva, N. Vitureira and myself thank the 53 authors that wrote the 15 articles on cutting-edge research connecting Uruguay with the world. A tribute to the founders of @socneurouy.bsky.social, but also to the 100 years of neuroscience history in Uruguay.