10/✅ We thank all colleagues and institutions for this collaborative effort.

@ufesoficial.bsky.social
@oficialfiocruz.bsky.social
@minsaude.bsky.social
@lagev-ufes.bsky.social
This study was funded by
#FAPES #SESA-ES
@cnpq-oficial.bsky.social

9/🔍 Our findings demonstrate the importance of integrated approaches — combining genomic, epidemiological, and ecological data — to address emerging arboviruses.

8/⚠️ This study highlights OROV's potential to expand beyond the Amazon, driven by environmental change, human mobility, and vector adaptability.
Strengthening surveillance is essential to mitigate future risks.

7/ 🌱🍫☕️🌶 Spatial analysis showed strong correlations between OROV incidence and agriculture.
Municipalities with extensive cocoa, coffee, and pepper cultivation were particularly affected — likely due to vector breeding habitats.

6/🌡️ Patients with fever, headache, and myalgia had significantly higher viral loads, indicated by lower Ct values in RT-qPCR assays.
This may suggest an association between viral replication and symptom severity.

5/🧍‍♂️🧍‍♀️ Most cases were adult males, likely reflecting occupational exposure in rural areas.
Common symptoms: fever (91%), headache (81%), muscle pain (68%), and retro-orbital pain (33%).

4/ 📈 The outbreak showed rapid transmission: the time-varying reproduction number (Rt) peaked at ~3.0, similar to urban arboviruses, like Dengue and Zika.

3/🔬We tested over 29,000 samples from patients with suspected arbovirus infections.
Molecular analyses identified OROV in 17 municipalities. Sequencing confirmed strains from the Amazonian reassortant lineage.

2/🌳🌾 Espírito Santo, historically free from OROV, became a hotspot due to favorable environmental and agricultural conditions.
Most cases occurred in areas with intensive cocoa, robusta coffee, coconut, and pepper farming, ideal for Culicoides paraensis.

We thank all co-authors and partner institutions involved in this multidisciplinary effort. In special to public health authorities, @ufesoficial.bsky.social, @oficialfiocruz.bsky.social, @lagev-ufes.bsky.social. This study was funded by Ministerio da Saúde, Governo do Espírito Santo e FAPES.

This study underscores how metabolic conditions (e.g., obesity, hepatic steatosis) and dysregulated immune responses may exacerbate OROV infection. Our findings call for enhanced surveillance, rapid diagnostics, and deeper research on viral evolution and host vulnerability.