Dr Geertje van Keulen
@drgvank.bsky.social
Swimming🏊♀️ sewciophilic🧵microbial 🧫biochemist🧪🧬/biomaterials ⚒️ scientist;
(bio)chemistry, materials and ecobiology of actinos/natural products;
microbiology/chemistry in hot tubs;
Biofilm Alliance;
Advisory Council member Microbiology Society;
She/her
(bio)chemistry, materials and ecobiology of actinos/natural products;
microbiology/chemistry in hot tubs;
Biofilm Alliance;
Advisory Council member Microbiology Society;
She/her
Ta!
5 ul spores at which density?
5 ul spores at which density?
October 14, 2025 at 5:35 PM
Ta!
5 ul spores at which density?
5 ul spores at which density?
Not open access alas...
September 21, 2025 at 10:40 AM
Not open access alas...
The Dutch word for constant is also starting with a k!
September 17, 2025 at 3:12 PM
The Dutch word for constant is also starting with a k!
Lots of integrative plasmids are linear. Streptomyces Ecoli shuttle plasmids (circular, often low cooy) allow cloning in Ecoli before moving into Streptomyces
September 13, 2025 at 3:56 PM
Lots of integrative plasmids are linear. Streptomyces Ecoli shuttle plasmids (circular, often low cooy) allow cloning in Ecoli before moving into Streptomyces
Streptomyces is not suitable for chemical or electro-transformation methods
September 12, 2025 at 1:12 PM
Streptomyces is not suitable for chemical or electro-transformation methods
Yeah, integrating plasmids can be quite big indeed. If they contain a Tra element than they can be conjugated. This sometimes needs Ecoli strain changeover if host has a methylated DNA restriction system. Plasmids without conjugative element can be moved into Streptomyces via protoplasting
September 12, 2025 at 1:11 PM
Yeah, integrating plasmids can be quite big indeed. If they contain a Tra element than they can be conjugated. This sometimes needs Ecoli strain changeover if host has a methylated DNA restriction system. Plasmids without conjugative element can be moved into Streptomyces via protoplasting
Could be integrating plasmids? Streptomyces uses this a lot
September 11, 2025 at 7:29 PM
Could be integrating plasmids? Streptomyces uses this a lot
Follow us on LinkedIn www.linkedin.com/company/biofilm-alliance/
September 10, 2025 at 6:33 PM
Follow us on LinkedIn www.linkedin.com/company/biofilm-alliance/
More info on the Biofilm Alliance here: www.biofilm-alliance.org
September 10, 2025 at 6:29 PM
More info on the Biofilm Alliance here: www.biofilm-alliance.org
Is that a Pioreactor?
August 15, 2025 at 5:06 PM
Is that a Pioreactor?