Chris Brooke
@christopherbrooke.bsky.social
Viral evolution and infection biology. Assoc. professor at University of Illinois at Urbana-Champaign. brookelab.org
11) We targeted each of the top hits and found that OASL knockdown resulted in a significant reduction in IFN induction after IAV infection. This was also true when we blocked OASL up-regulation.
March 18, 2025 at 6:47 PM
11) We targeted each of the top hits and found that OASL knockdown resulted in a significant reduction in IFN induction after IAV infection. This was also true when we blocked OASL up-regulation.
10) It turns out that a subset of cells within any given cell population express these ISGs in the absence of stimuli.
This was true both in transformed cell lines as well as in primary human bronchial epithelial cells differentiated at the air-liquid interface.
This was true both in transformed cell lines as well as in primary human bronchial epithelial cells differentiated at the air-liquid interface.
March 18, 2025 at 6:47 PM
10) It turns out that a subset of cells within any given cell population express these ISGs in the absence of stimuli.
This was true both in transformed cell lines as well as in primary human bronchial epithelial cells differentiated at the air-liquid interface.
This was true both in transformed cell lines as well as in primary human bronchial epithelial cells differentiated at the air-liquid interface.
9) To our surprise, all the top hits were well known interferon stimulated genes (ISGs)...genes that are most famous for getting turned on in response to IFN.
Why were ISGs showing up in uninfected/unstimulated cells as correlates of IFN induction potential?
Why were ISGs showing up in uninfected/unstimulated cells as correlates of IFN induction potential?
March 18, 2025 at 6:47 PM
9) To our surprise, all the top hits were well known interferon stimulated genes (ISGs)...genes that are most famous for getting turned on in response to IFN.
Why were ISGs showing up in uninfected/unstimulated cells as correlates of IFN induction potential?
Why were ISGs showing up in uninfected/unstimulated cells as correlates of IFN induction potential?
7) This approach allowed us to reconstruct how individual cells move through transcriptional state space from pre-infection to post-infection states:
March 18, 2025 at 6:47 PM
7) This approach allowed us to reconstruct how individual cells move through transcriptional state space from pre-infection to post-infection states:
2) Many groups (including ours) have shown that the interferon (IFN) response is generally initiated by a small subset of cells during infection with IAV or other viruses.
The rarity of IFN+ cells during infection is a bit of a surprise given the critical importance of IFN for host defense.
The rarity of IFN+ cells during infection is a bit of a surprise given the critical importance of IFN for host defense.
March 18, 2025 at 6:47 PM
2) Many groups (including ours) have shown that the interferon (IFN) response is generally initiated by a small subset of cells during infection with IAV or other viruses.
The rarity of IFN+ cells during infection is a bit of a surprise given the critical importance of IFN for host defense.
The rarity of IFN+ cells during infection is a bit of a surprise given the critical importance of IFN for host defense.
We have an open faculty search for TWO positions in microbiology and virology at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty...you could be next!
Please re-post/skeet!
Please re-post/skeet!
September 9, 2024 at 8:36 PM
We have an open faculty search for TWO positions in microbiology and virology at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty...you could be next!
Please re-post/skeet!
Please re-post/skeet!
Excited to welcome @themenacherylab.bsky.social here to Illinois to give a seminar in the only way that seems right:
October 5, 2023 at 6:04 PM
Excited to welcome @themenacherylab.bsky.social here to Illinois to give a seminar in the only way that seems right:
We have an open virology faculty search at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty working on cutting edge questions in virus biology...you could be next!
Please re-post/RT/re-bleet/whatever it is called here!
Please re-post/RT/re-bleet/whatever it is called here!
September 5, 2023 at 2:35 PM
We have an open virology faculty search at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty working on cutting edge questions in virus biology...you could be next!
Please re-post/RT/re-bleet/whatever it is called here!
Please re-post/RT/re-bleet/whatever it is called here!