Cameron Dale MacQuarrie
@cdmacquarrie.bsky.social
Scientist at Arcadia Science
Berkeley, CA, USA
Cell Biology
He/Him
Berkeley, CA, USA
Cell Biology
He/Him
Third, after 47 days, cpc1-1 was the ONLY strain that could grow on marine medium with high salt content, revealing an unexpected halotolerance. [6/9]
May 22, 2025 at 8:23 PM
Third, after 47 days, cpc1-1 was the ONLY strain that could grow on marine medium with high salt content, revealing an unexpected halotolerance. [6/9]
First, cpc1-1 showed enhanced growth on media with proteose peptone, outperforming wild-type strains that barely grew in this condition! [4/9]
May 22, 2025 at 8:23 PM
First, cpc1-1 showed enhanced growth on media with proteose peptone, outperforming wild-type strains that barely grew in this condition! [4/9]
Today at #CellBio24, I'll discuss our new pub at the "Quantitative Biology in Emerging Model Systems" subgroup session at 5:15 pm in Ballroom 20A. Come check it out!
December 17, 2024 at 7:03 PM
Today at #CellBio24, I'll discuss our new pub at the "Quantitative Biology in Emerging Model Systems" subgroup session at 5:15 pm in Ballroom 20A. Come check it out!
Despite similar motility defects, the mutations responded differently to treatment —- what rescued one mutation often had no effect on the other. This suggests mutation-specific therapeutic approaches are needed [5/8]
December 16, 2024 at 5:22 PM
Despite similar motility defects, the mutations responded differently to treatment —- what rescued one mutation often had no effect on the other. This suggests mutation-specific therapeutic approaches are needed [5/8]
We developed two complementary approaches to measure motility: a population "sink-or-swim" assay and detailed single-cell tracking with SwimTracker (research.arcadiascience.com/pub/resource-swimtracker-htp-swimming-assay/release/2). This revealed how drugs affect different aspects of swimming [4/8]
December 16, 2024 at 5:22 PM
We developed two complementary approaches to measure motility: a population "sink-or-swim" assay and detailed single-cell tracking with SwimTracker (research.arcadiascience.com/pub/resource-swimtracker-htp-swimming-assay/release/2). This revealed how drugs affect different aspects of swimming [4/8]
These mutants were characterized decades ago and show similar swimming defects to those seen in SPGF patients' sperm. Our phylogenetic framework showed they're excellent models for SPGF83 and SPGF43 [3/8]
December 16, 2024 at 5:22 PM
These mutants were characterized decades ago and show similar swimming defects to those seen in SPGF patients' sperm. Our phylogenetic framework showed they're excellent models for SPGF83 and SPGF43 [3/8]
We used phylogenetic analysis from our pub led by Ryan York, Austin Patton & @pracheeac.bsky.social (research.arcadiascience.com/pub/result-evolutionary-organismal-selection) and found Chlamydomonas proteins have high conservation with SPGF proteins. [2/8]
December 16, 2024 at 5:22 PM
We used phylogenetic analysis from our pub led by Ryan York, Austin Patton & @pracheeac.bsky.social (research.arcadiascience.com/pub/result-evolutionary-organismal-selection) and found Chlamydomonas proteins have high conservation with SPGF proteins. [2/8]
Tomorrow at #CellBio24, I'll be at poster (B73) talking about some fun new work we just released using Chlamydomonas to model spermatogenic failure.
Check out the poster here: zenodo.org/records/14481493
Join the fun and post your comments & questions on the pub!
bit.ly/chlamy-spgf
Check out the poster here: zenodo.org/records/14481493
Join the fun and post your comments & questions on the pub!
bit.ly/chlamy-spgf
December 15, 2024 at 9:25 PM
Tomorrow at #CellBio24, I'll be at poster (B73) talking about some fun new work we just released using Chlamydomonas to model spermatogenic failure.
Check out the poster here: zenodo.org/records/14481493
Join the fun and post your comments & questions on the pub!
bit.ly/chlamy-spgf
Check out the poster here: zenodo.org/records/14481493
Join the fun and post your comments & questions on the pub!
bit.ly/chlamy-spgf
We designed a 96-well plate magnetic field block. Surprisingly, after a week of growth on the block, we were able to clearly distinguish between these two species, consistent with C. smithii having a thinner cell wall! 7/9
December 13, 2023 at 7:10 PM
We designed a 96-well plate magnetic field block. Surprisingly, after a week of growth on the block, we were able to clearly distinguish between these two species, consistent with C. smithii having a thinner cell wall! 7/9
But this method is low-throughput, so instead, we grew cells w/ Congo red, which is toxic to cell wall (cw) mutant yeast. It wasn’t sensitive enough to distinguish btw the two WT species, but we saw less growth in cw mutants, so this is a decent assay to find algal cw mutants 5/9
December 13, 2023 at 7:08 PM
But this method is low-throughput, so instead, we grew cells w/ Congo red, which is toxic to cell wall (cw) mutant yeast. It wasn’t sensitive enough to distinguish btw the two WT species, but we saw less growth in cw mutants, so this is a decent assay to find algal cw mutants 5/9
We started by staining the two species with calcofluor-white, which binds to cell wall components. Our analysis showed thicker staining in C. reinhardtii, consistent with C. smithii having a weaker wall (analysis in the pub!)! 4/9
December 13, 2023 at 7:07 PM
We started by staining the two species with calcofluor-white, which binds to cell wall components. Our analysis showed thicker staining in C. reinhardtii, consistent with C. smithii having a weaker wall (analysis in the pub!)! 4/9
We’d also found that C. smithii is more sensitive to detergent, which could indicate less environmental protection. We’ve adapted this protocol to work in a 96-well plate. These findings led us to compare the cell wall of the species. 3/9
December 13, 2023 at 7:06 PM
We’d also found that C. smithii is more sensitive to detergent, which could indicate less environmental protection. We’ve adapted this protocol to work in a 96-well plate. These findings led us to compare the cell wall of the species. 3/9
First update! Although we’ve seen C. smithii adapting to a wide range of media, these cells are more sensitive to a harsh antibiotic-fungicide cocktail! 2/9
December 13, 2023 at 7:05 PM
First update! Although we’ve seen C. smithii adapting to a wide range of media, these cells are more sensitive to a harsh antibiotic-fungicide cocktail! 2/9
🎉 Excited to share our latest work at #CellBio23! I'll be presenting a poster on our pub “Phenotypic differences between interfertile Chlamydomonas species” and our continued work in the Protists and Parasites Cell Biology poster session (Board #723) @arcadiascience.bsky.social
December 1, 2023 at 9:28 PM
🎉 Excited to share our latest work at #CellBio23! I'll be presenting a poster on our pub “Phenotypic differences between interfertile Chlamydomonas species” and our continued work in the Protists and Parasites Cell Biology poster session (Board #723) @arcadiascience.bsky.social
DALL-E's take on a colored pencil sketch of Chlamydomonas
November 11, 2023 at 4:35 AM
DALL-E's take on a colored pencil sketch of Chlamydomonas
While the size differs, the roundness or 'eccentricity' of cells across both species is similar. This baseline measurement may become relevant if we see deviations from the parental shape among the hybrid progeny.
September 1, 2023 at 9:48 PM
While the size differs, the roundness or 'eccentricity' of cells across both species is similar. This baseline measurement may become relevant if we see deviations from the parental shape among the hybrid progeny.
Our update reveals that C. smithii cells are 21% larger than C. reinhardtii. We’ll consider this baseline difference when we assess the phenotypes of hybrid progeny.
September 1, 2023 at 9:47 PM
Our update reveals that C. smithii cells are 21% larger than C. reinhardtii. We’ll consider this baseline difference when we assess the phenotypes of hybrid progeny.
New pub update from @arcadiascience.bsky.social! ! We've just released an update to our research on the 2D cell morphology of C. reinhardtii and C. smithii, two interfertile algal species.
research.arcadiascience.com/pub/result-c...
research.arcadiascience.com/pub/result-c...
September 1, 2023 at 9:47 PM
New pub update from @arcadiascience.bsky.social! ! We've just released an update to our research on the 2D cell morphology of C. reinhardtii and C. smithii, two interfertile algal species.
research.arcadiascience.com/pub/result-c...
research.arcadiascience.com/pub/result-c...
