Dr Benjamin Sharpe
@bsharpenotflat.bsky.social
Cancer cell biologist and methods development scientist: organoids, histology, imaging and image analysis. Postdoc at University of Southampton Cancer Sciences with @timfenton10.bsky.social
I've heard it called a "pedigree" before at conferences and the whole idea of it makes me sad to be honest. We're supposed to be smart people, you would think we would know better!
October 23, 2025 at 7:28 AM
I've heard it called a "pedigree" before at conferences and the whole idea of it makes me sad to be honest. We're supposed to be smart people, you would think we would know better!
pmc.ncbi.nlm.nih.gov/articles/PMC...
The dye is a pain to prepare with serial extraction in chloroform but once you've done it, you'll have enough for several lifetimes!
The dye is a pain to prepare with serial extraction in chloroform but once you've done it, you'll have enough for several lifetimes!
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate t...
pmc.ncbi.nlm.nih.gov
September 12, 2025 at 12:21 PM
pmc.ncbi.nlm.nih.gov/articles/PMC...
The dye is a pain to prepare with serial extraction in chloroform but once you've done it, you'll have enough for several lifetimes!
The dye is a pain to prepare with serial extraction in chloroform but once you've done it, you'll have enough for several lifetimes!
Honestly I'm really loving methyl green. You don't have to use much, it's really bright and you can excite it with 633nm laser so it doesn't bleed through into 488 as badly as DAPI can
September 12, 2025 at 11:22 AM
Honestly I'm really loving methyl green. You don't have to use much, it's really bright and you can excite it with 633nm laser so it doesn't bleed through into 488 as badly as DAPI can
Happy Christmas Tim 🥰
December 16, 2024 at 8:27 PM
Happy Christmas Tim 🥰