Great question, Noam! Depends a bit on the cycle segment, and how we define "% of biomass flux". In our final implementation (acetoacetate as main carbon source), "module 3" of the cycle carries 100% biomass flux. The other modules carry 8% (when normalized to acetoacetate influx). FBA estimation:

To show that our metabolic designs can make it from the drawing board to an actual living cell, we used growth-coupled selection and adaptive evolution in E.coli.
Breaking the cyclic pathway down into short, testable “modules” was key for this strategy. 🧩🔄🗝️

Excited to share a main project from my PhD, out now in
@naturecomms.bsky.social! 📝
We've designed and brought to life the “CORE cycle” – a new-to-nature pathway that provides a novel route for biological CO2 capture 🦠🌱
nature.com/articles/s41...
Take a look! Thread below... 🧵