Analytical and Quantitative Light Microscopy Course
aqlm.bsky.social
Analytical and Quantitative Light Microscopy Course
@aqlm.bsky.social
This is the account for the Analytical and Quantitative Light Microscopy Course at the Marine Biological Laboratory in Woods Hole MA. We love all things microscopy!
Day 3 begins our dive into #fluorscence #microscopy with a lab and lecture from none other than Dr. Wendy Salmon, @fishcscells.bsky.social, director of the Hooker Imaging Core @UNC. She teaches us about the fluorescence #light path, filters, and #fluorophores!

Apply here- bit.ly/49YQl7q
December 6, 2025 at 2:02 PM
Day 3 of AQLM also finds us learning about point spread functions from Dr. Keith Lidke. Keith hails from the #Physics and Astronomy Department at UNM, and lectures us on the physics of PSFs-- a crucial background for all the advanced #imaging topics to come.

Apply now- bit.ly/49YQl7q
#microscopy
December 4, 2025 at 6:47 PM
Day 3 begins with a lecture and lab on light #microscopy (#phase, #polarization, #DIC) from the outstanding Dr. Shalin Mehta, @mattersoflight.bsky.social. New this year, we will incorporate an additional exercise on quantitative phase imaging!

Apply now- bit.ly/49YQl7q
December 2, 2025 at 6:44 PM
Day 2 also covers Fourier Optics, with a fantastic lecture from Dr. Peter Kner! You'll never understand Fourier transforms better than you do after Peter explains it, trust us.

Apply now to join us and finally understand #Fourier space! bit.ly/49YQl7q

#microscopy
November 28, 2025 at 8:40 PM
On the second day of AQLM, we also have Dr. Steve Ross of #Nikon Instruments who gives us an inside look at #optics, objective #lenses, and #image aberrations. You've (hopefully) never seen an objective like this before!

Apply now to join us April 29th-May 8th 2026. bit.ly/49YQl7q
#microscopy
November 24, 2025 at 11:01 PM
Next on the 2026 roster is Dr. Peter Kner, @knerlab.bsky.social. He lectures on wave #optics, #diffraction, and #contrast generation in imaging. This Physics 101 gets everyone on the same #wavelength, even if your background is all biology. Apply now to join us! bit.ly/49YQl7q

#microscopy
November 22, 2025 at 2:15 PM
First up is AQLM Director, Dr. Alexa Mattheyses. @mattheyseslab.bsky.social She kicks off the course with an introduction to #optics, where you build a #Kohler #microscope using nothing but #lenses and #physics.

If this sounds like your jam, apply now to join! bit.ly/49YQl7q
#microscopy
November 20, 2025 at 1:20 PM
AQLM 2026 features an amazing selection of faculty who are truly experts in their fields. Their commitment to education and supporting AQLM make the course the outstanding experience it is, so we're going to highlight each of them over the next few weeks.

Apply now! bit.ly/49YQl7q

#microscopy
November 18, 2025 at 4:16 PM
Applications are now open for AQLM 2026! Join us @mblscience.bsky.social April 29th-May 8th, 2026 for a comprehensive, hands-on light microscopy course with an amazing roster of faculty who are leading experts in their fields!
November 7, 2025 at 2:29 PM
And just like that, #AQLM2025 is over. Hopefully we’ll all find ourselves in the same windowless microscope room sometime soon, bead slide in hand.
May 9, 2025 at 1:42 PM
This year’s esteemed Inoue Lecture was given by French optical physicist Dr. Sophie Brasselet of The Fresnel Institute. Dr. Brasselet spoke of her team’s efforts towards applying 6 degrees of PSF engineering to super resolution polarization microscopy. #AQLM2025
May 9, 2025 at 1:43 AM
As a final exercise everyone got to build open source microscopes using kits available from UC2 @openuc2.bsky.social #AQLM2025
May 9, 2025 at 1:28 AM
The machines learned this morning. Dr. Daniel Cortes led a lab on machine learning for image processing.
May 8, 2025 at 7:33 PM
Arabidopsis labeled with EB1b-mNeon in green (microtubule plus end) and tubulin in magenta (microtubules). Timelapse image on the Leica Stellaris 8 laser scanning confocal. @leicamicrosystems.bsky.social @mblscience.bsky.social #AQLM2025
May 6, 2025 at 11:10 PM
Tracking Arabidopsis microtubule tips on the NSPARC 🌈
May 5, 2025 at 9:28 PM
Arabidopsis with EB1b-mNEON labeling the plus end of microtubules 🌱 Imaged on the Nikon AX-R laser scanning confocal with NSPARC. #AQLM2025
May 5, 2025 at 8:47 PM
Live cell lab: understanding the trade offs between acquisition time, SNR, spatial resolution, and cell survival on different microscope systems. You can’t have it all, but ideally you can have enough for meaningful analysis. #AQLM2025
May 5, 2025 at 7:13 PM
The back focal plane! Today Dr. Chris Bjornsson demonstrated Abbe’s theory of resolution by manipulating the light passing through the back focal plane. Here he is using a lens to focus the back focal plane onto the camera for us to see. #AQLM2025
May 4, 2025 at 9:59 PM
Dr. Shalin Mehta giving a tour of Shinya Inoue’s polarization scope housed in Lillie @mblscience.bsky.social 🔬
#AQLM2025
May 4, 2025 at 6:53 PM
FRET tonight! This is the Leica Stellaris 8, part of the MBL Imaging Innovation Center. #AQLM2025
May 4, 2025 at 3:03 AM
Watch how the color shifts as the angle changes.
#AQLM2025
May 4, 2025 at 2:59 AM
Sugar under polarized light 🤩
#AQLM2025
May 3, 2025 at 11:38 PM
Rabbit muscle fibers under darkfield. Something interesting is happening where two fibers overlap…
#AQLM2025
May 3, 2025 at 11:35 PM
Fluorescence lab is upon us. Time to get to know (and love) beads. #AQLM2025
May 2, 2025 at 3:54 PM
Have you ever seen the black box that is the inside of a microscope objective? Today Dr. Stephen Ross of Nikon gave a fascinating lecture on what goes into the design and manufacturing of an objective.
May 1, 2025 at 6:16 PM