Anzy Miller
@anzymiller.bsky.social
Interested in how protein dynamics affect cell fate decisions. #DevBio #StemCells #Oscillations #Epigenetics @uniofmanchester.bsky.social
Big thanks to all authors and the funders! @veronicabiga.bsky.social @papalopululab.bsky.social @wellcometrust.bsky.social @erc.europa.eu @ukri.org
July 3, 2025 at 1:39 PM
Big thanks to all authors and the funders! @veronicabiga.bsky.social @papalopululab.bsky.social @wellcometrust.bsky.social @erc.europa.eu @ukri.org
4. The fold-change of the oscillations are read through an incoherent feed-forward (IFFL) gene regulatory motif. NGN3 oscillations allow for stochasticity in fold-change over time, resulting in the spreading out of differentiation over time.
July 3, 2025 at 1:35 PM
4. The fold-change of the oscillations are read through an incoherent feed-forward (IFFL) gene regulatory motif. NGN3 oscillations allow for stochasticity in fold-change over time, resulting in the spreading out of differentiation over time.
3. NGN3 oscillatory dynamics are decoded via the fold-change not expression level
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
July 3, 2025 at 1:18 PM
3. NGN3 oscillatory dynamics are decoded via the fold-change not expression level
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
2. Non-oscillatory NGN3 results in earlier differentiation
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
July 3, 2025 at 1:17 PM
2. Non-oscillatory NGN3 results in earlier differentiation
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
1. NGN3 protein expression is oscillatory in human pancreatic endocrine progenitors
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
July 3, 2025 at 1:15 PM
1. NGN3 protein expression is oscillatory in human pancreatic endocrine progenitors
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.