Andrew Krusenstjerna, Ph.D.
@ackrusen.bsky.social
Chicano | Microbiologist | Ph.D. w/ @BStevensonLab | Postdoc w/ @GoleyLab | Appreciating the little things in life | Views are my own 🧫🇲🇽🏳️🌈(He/Him)
Reposted by Andrew Krusenstjerna, Ph.D.
Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC url: academic.oup.com/nar/article/...
Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC
Abstract. Natural chromosomal transformation (NCT) in Bacillus subtilis requires RecA and its accessory proteins including RecX and RecD2. Inactivation of
academic.oup.com
October 25, 2025 at 12:10 PM
Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC url: academic.oup.com/nar/article/...
New paper from the Voss lab!
RLip, a secreted lipase, helps pathogenic Rickettsia thrive inside host cells. Grateful our work in the Goley lab building a R. parkeri transposon library could support this discovery. 🦠 🔬
journals.plos.org/plosone/arti...
RLip, a secreted lipase, helps pathogenic Rickettsia thrive inside host cells. Grateful our work in the Goley lab building a R. parkeri transposon library could support this discovery. 🦠 🔬
journals.plos.org/plosone/arti...
Pathogenic rickettsiae encode a secreted lipase that facilitates intracytosolic colonization in host cells
Key cellular processes for the rickettsial obligate intracellular lifestyle, including internalization by phagocytosis, regulation of intracellular trafficking, and evasion of lysosomal destruction to...
journals.plos.org
October 11, 2025 at 11:41 PM
New paper from the Voss lab!
RLip, a secreted lipase, helps pathogenic Rickettsia thrive inside host cells. Grateful our work in the Goley lab building a R. parkeri transposon library could support this discovery. 🦠 🔬
journals.plos.org/plosone/arti...
RLip, a secreted lipase, helps pathogenic Rickettsia thrive inside host cells. Grateful our work in the Goley lab building a R. parkeri transposon library could support this discovery. 🦠 🔬
journals.plos.org/plosone/arti...
Reposted by Andrew Krusenstjerna, Ph.D.
New PubPeer comment on "Efficacy of oral folinic acid supplementation in children with autism spectrum disorder: a randomized double-blind, placebo-controlled trial" (Panda et al, 2024). Concerns about significant errors. #folinicacid #leucovorin, #autism
pubpeer.com/publications...
pubpeer.com/publications...
PubPeer - Efficacy of oral folinic acid supplementation in children wi...
There are comments on PubPeer for publication: Efficacy of oral folinic acid supplementation in children with autism spectrum disorder: a randomized double-blind, placebo-controlled trial (2024)
pubpeer.com
September 27, 2025 at 2:20 AM
New PubPeer comment on "Efficacy of oral folinic acid supplementation in children with autism spectrum disorder: a randomized double-blind, placebo-controlled trial" (Panda et al, 2024). Concerns about significant errors. #folinicacid #leucovorin, #autism
pubpeer.com/publications...
pubpeer.com/publications...
Reposted by Andrew Krusenstjerna, Ph.D.
In #mBio, dive into story behind the 1989 discovery of Helicobacter pylori CagA, the first tumorigenic bacterial protein. This article recounts the early steps in the investigative process and relates some of the unexpected discoveries that ensued. asm.social/2C1
September 24, 2025 at 6:13 PM
In #mBio, dive into story behind the 1989 discovery of Helicobacter pylori CagA, the first tumorigenic bacterial protein. This article recounts the early steps in the investigative process and relates some of the unexpected discoveries that ensued. asm.social/2C1
Reposted by Andrew Krusenstjerna, Ph.D.
If you want to get started, here's a tutorial that I made:
github.com/JoachimGoedh...
Reach out if you have questions or want advice!!
github.com/JoachimGoedh...
Reach out if you have questions or want advice!!
GitHub - JoachimGoedhart/A_Shiny_start: A beginners tutorial on Shiny apps
A beginners tutorial on Shiny apps. Contribute to JoachimGoedhart/A_Shiny_start development by creating an account on GitHub.
github.com
September 15, 2025 at 5:38 PM
If you want to get started, here's a tutorial that I made:
github.com/JoachimGoedh...
Reach out if you have questions or want advice!!
github.com/JoachimGoedh...
Reach out if you have questions or want advice!!
Reposted by Andrew Krusenstjerna, Ph.D.
2025 #LaskerAward winner Lucy Shapiro asked: How do living organisms translate information from a linear genetic code into three-dimensional structures?
And with that, she broke open a new field. 🧪
@pnas.org
#Lasker2025 #systemsbiology
And with that, she broke open a new field. 🧪
@pnas.org
#Lasker2025 #systemsbiology
The Lasker~Koshland Special Achievement Award in Medical Science awarded to Lucy Shapiro | PNAS
Scientists can contribute to society in numerous ways. Some scientists discover new biological principles and found entirely new fields. Some scien...
www.pnas.org
September 15, 2025 at 4:45 PM
2025 #LaskerAward winner Lucy Shapiro asked: How do living organisms translate information from a linear genetic code into three-dimensional structures?
And with that, she broke open a new field. 🧪
@pnas.org
#Lasker2025 #systemsbiology
And with that, she broke open a new field. 🧪
@pnas.org
#Lasker2025 #systemsbiology
Reposted by Andrew Krusenstjerna, Ph.D.
Reposted by Andrew Krusenstjerna, Ph.D.
Some awesome, fundamental work here: Lucy Shapiro, on bacterial cell differentiation, Dirk Görlich and Steve McKnight on IDRs & protein gels in nuclear pores and biomolecular condensates, & Michael Welsh, Jesús González & Paul Negulescu, (overdue) for bench to bedside work on cystic fibrosis 🧪 1/2
This year's Lasker Awards went to scientists who studied the wiring diagram of life, a new state of biological matter, and a potent treatment for cystic fibrosis. Here's my story with Gina Kolata. Gift link: nyti.ms/4mZlH1F
nyti.ms
September 11, 2025 at 11:20 PM
Some awesome, fundamental work here: Lucy Shapiro, on bacterial cell differentiation, Dirk Görlich and Steve McKnight on IDRs & protein gels in nuclear pores and biomolecular condensates, & Michael Welsh, Jesús González & Paul Negulescu, (overdue) for bench to bedside work on cystic fibrosis 🧪 1/2
Reposted by Andrew Krusenstjerna, Ph.D.
This year's Lasker Awards went to scientists who studied the wiring diagram of life, a new state of biological matter, and a potent treatment for cystic fibrosis. Here's my story with Gina Kolata. Gift link: nyti.ms/4mZlH1F
nyti.ms
September 11, 2025 at 1:30 PM
This year's Lasker Awards went to scientists who studied the wiring diagram of life, a new state of biological matter, and a potent treatment for cystic fibrosis. Here's my story with Gina Kolata. Gift link: nyti.ms/4mZlH1F
Reposted by Andrew Krusenstjerna, Ph.D.
Regulation by RNAs might be even more extensive than we thought. Many enzymes and other proteins not previously considered as RNA-binding proteins do seem to fall into this class & might have their activity regulated by RNA.
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Rethinking RNA-binding proteins: Riboregulation challenges prevailing views
The advent of system-wide proteomic approaches has largely expanded the number of
RNA-binding proteins (RBPs). This review discusses how recent discoveries are transforming
our understanding of biolog...
www.cell.com
September 5, 2025 at 3:49 PM
Regulation by RNAs might be even more extensive than we thought. Many enzymes and other proteins not previously considered as RNA-binding proteins do seem to fall into this class & might have their activity regulated by RNA.
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Andrew Krusenstjerna, Ph.D.
New in JB: Gbolahan, Saxena et al. link membrane stress, the Rcs stress-response pathway and a block in chromosomal replication to the targeting of the DnaA initiator, likely via the ClpP protease.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
September 5, 2025 at 12:29 PM
New in JB: Gbolahan, Saxena et al. link membrane stress, the Rcs stress-response pathway and a block in chromosomal replication to the targeting of the DnaA initiator, likely via the ClpP protease.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Reposted by Andrew Krusenstjerna, Ph.D.
Our newest preprint on bioRxiv @biorxivpreprint.bsky.social "Divergent Rickettsia species exhibit distinct mechanisms of actin-based motility" reveals surprising evolutionary flexibility in the mechanisms of Rickettsia actin-based motility www.biorxiv.org/content/10.1...
August 22, 2025 at 7:16 PM
Our newest preprint on bioRxiv @biorxivpreprint.bsky.social "Divergent Rickettsia species exhibit distinct mechanisms of actin-based motility" reveals surprising evolutionary flexibility in the mechanisms of Rickettsia actin-based motility www.biorxiv.org/content/10.1...
Reposted by Andrew Krusenstjerna, Ph.D.
Reposted by Andrew Krusenstjerna, Ph.D.
The 2024 rankings of bacterial threats
August 27, 2025 at 4:19 PM
The 2024 rankings of bacterial threats
Reposted by Andrew Krusenstjerna, Ph.D.
🚨 Job alert 🚨
I’m recruiting two postdoctoral research associates to join my lab at KCL to study how #Klebsiella pneumoniae regulates virulence factor expression during infection! #klebclub
Mol Micro: tinyurl.com/mtrwtx5e
Infection: tinyurl.com/2s3u8ca2
Deadline: 21st September.
I’m recruiting two postdoctoral research associates to join my lab at KCL to study how #Klebsiella pneumoniae regulates virulence factor expression during infection! #klebclub
Mol Micro: tinyurl.com/mtrwtx5e
Infection: tinyurl.com/2s3u8ca2
Deadline: 21st September.
Postdoctoral Research Associate in Molecular Microbiology
www.kcl.ac.uk
August 26, 2025 at 2:40 PM
🚨 Job alert 🚨
I’m recruiting two postdoctoral research associates to join my lab at KCL to study how #Klebsiella pneumoniae regulates virulence factor expression during infection! #klebclub
Mol Micro: tinyurl.com/mtrwtx5e
Infection: tinyurl.com/2s3u8ca2
Deadline: 21st September.
I’m recruiting two postdoctoral research associates to join my lab at KCL to study how #Klebsiella pneumoniae regulates virulence factor expression during infection! #klebclub
Mol Micro: tinyurl.com/mtrwtx5e
Infection: tinyurl.com/2s3u8ca2
Deadline: 21st September.
Reposted by Andrew Krusenstjerna, Ph.D.
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.
www.nature.com
July 29, 2025 at 1:23 PM
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
Reposted by Andrew Krusenstjerna, Ph.D.
Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @cp-cell.bsky.social, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. www.sciencedirect.com/science/arti...
July 26, 2025 at 7:15 PM
Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @cp-cell.bsky.social, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. www.sciencedirect.com/science/arti...
Reposted by Andrew Krusenstjerna, Ph.D.
Update on North American tick-borne diseases and how to diagnose them
Kyle Rodino, Eli Theel, Bobby Pritt
journals.asm.org/doi/10.1128/...
Kyle Rodino, Eli Theel, Bobby Pritt
journals.asm.org/doi/10.1128/...
July 20, 2025 at 6:47 PM
Update on North American tick-borne diseases and how to diagnose them
Kyle Rodino, Eli Theel, Bobby Pritt
journals.asm.org/doi/10.1128/...
Kyle Rodino, Eli Theel, Bobby Pritt
journals.asm.org/doi/10.1128/...
Reposted by Andrew Krusenstjerna, Ph.D.
#phage #phagesky
A bacterial host factor confines phage localization for excluding the infected compartment through cell division: www.cell.com/cell-reports...
A bacterial host factor confines phage localization for excluding the infected compartment through cell division: www.cell.com/cell-reports...
A bacterial host factor confines phage localization for excluding the infected compartment through cell division
Pollak Fiyaksel et al. describe YjbH, a helix-turn-helix DNA-binding protein in Bacillus
subtilis, conserved among gram-positive bacteria. YjbH binds the incoming phage genome,
confines phage producti...
www.cell.com
July 17, 2025 at 1:08 PM
#phage #phagesky
A bacterial host factor confines phage localization for excluding the infected compartment through cell division: www.cell.com/cell-reports...
A bacterial host factor confines phage localization for excluding the infected compartment through cell division: www.cell.com/cell-reports...
Reposted by Andrew Krusenstjerna, Ph.D.
Excited to share our new paper in @cellreports.bsky.social that reshapes our understanding of chromosome organization's deep evolutionary roots! Our work dives into the origins of the machinery that structures our very genomes.
🔗: doi.org/10.1016/j.ce...
#Genomics #Evolution #CellBiology #LECA
🔗: doi.org/10.1016/j.ce...
#Genomics #Evolution #CellBiology #LECA
June 22, 2025 at 12:15 AM
Excited to share our new paper in @cellreports.bsky.social that reshapes our understanding of chromosome organization's deep evolutionary roots! Our work dives into the origins of the machinery that structures our very genomes.
🔗: doi.org/10.1016/j.ce...
#Genomics #Evolution #CellBiology #LECA
🔗: doi.org/10.1016/j.ce...
#Genomics #Evolution #CellBiology #LECA
Reposted by Andrew Krusenstjerna, Ph.D.
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
June 17, 2025 at 12:20 PM
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Reposted by Andrew Krusenstjerna, Ph.D.
Ancient remains reveal how a pathogen began to use lice—not ticks—to infect humans, according to a new study in Science. scim.ag/3Zbkri6
Ancient Borrelia genomes document the evolutionary history of louse-borne relapsing fever
Several bacterial pathogens have transitioned from tick-borne to louse-borne transmission, which often involves genome reduction and increasing virulence. However, the timing of such transitions remai...
scim.ag
June 2, 2025 at 8:14 PM
Ancient remains reveal how a pathogen began to use lice—not ticks—to infect humans, according to a new study in Science. scim.ag/3Zbkri6
Reposted by Andrew Krusenstjerna, Ph.D.
🧵5 Top Free Alternatives to BioRender for Scientific Illustrations!
These five websites offer free scientific illustrations for biologists. Great for presentations, research papers and other research communication needs.
Save and share the post!
These five websites offer free scientific illustrations for biologists. Great for presentations, research papers and other research communication needs.
Save and share the post!
May 13, 2025 at 7:55 PM
🧵5 Top Free Alternatives to BioRender for Scientific Illustrations!
These five websites offer free scientific illustrations for biologists. Great for presentations, research papers and other research communication needs.
Save and share the post!
These five websites offer free scientific illustrations for biologists. Great for presentations, research papers and other research communication needs.
Save and share the post!
Reposted by Andrew Krusenstjerna, Ph.D.
I know you all know this, but apparently it needs to be said that basic science is a NECESSARY PRECURSOR to translational science
NSF to ONLY fund research in five areas: artificial intelligence, quantum information science, biotechnology, nuclear energy, and translational science. Can this be happening?
www.science.org/content/arti...
www.science.org/content/arti...
NSF slashes number of ‘rotators’ and well-paid managers as part of restructuring
Smaller future budgets will require fewer people, NSF official tells staff
www.science.org
May 9, 2025 at 11:57 PM
I know you all know this, but apparently it needs to be said that basic science is a NECESSARY PRECURSOR to translational science
Reposted by Andrew Krusenstjerna, Ph.D.
"Why don't you buy a new centrifuge"
Because everyone knows that any appliances in that shade of yellow will outlive you and your entire family, look at it
Because everyone knows that any appliances in that shade of yellow will outlive you and your entire family, look at it
March 20, 2025 at 6:06 PM
"Why don't you buy a new centrifuge"
Because everyone knows that any appliances in that shade of yellow will outlive you and your entire family, look at it
Because everyone knows that any appliances in that shade of yellow will outlive you and your entire family, look at it