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ProImmune @proimmune.bsky.social · 18d

This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.

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ProImmune @proimmune.bsky.social · Sep 12

This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron

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ProImmune @proimmune.bsky.social · May 2

If #FlowcytometryFriday is more your thing, then Ankyron™ AC40302 works well in this application, shown in HeLa (red histogram, with negative control of secondary fluorophore only in grey).

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ProImmune @proimmune.bsky.social · May 2

Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron

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ProImmune @proimmune.bsky.social · Apr 25

Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope.

#immunology #animalfree

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ProImmune @proimmune.bsky.social · Apr 7

#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron

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ProImmune @proimmune.bsky.social · Apr 4

This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron

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ProImmune @proimmune.bsky.social · Mar 28

We were happy to see that this Ankyron also works well in #FlowCytometry, with HUVEC stained with V5-tagged Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

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ProImmune @proimmune.bsky.social · Mar 28

Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope

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ProImmune @proimmune.bsky.social · Mar 21

This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social

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ProImmune @proimmune.bsky.social · Mar 14

#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social

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ProImmune @proimmune.bsky.social · Mar 13

Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.

doi.org/10.1208/s122...
#BLI #immunology #animalfree

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ProImmune @proimmune.bsky.social · Mar 7

It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs
@afruk.bsky.social

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ProImmune @proimmune.bsky.social · Mar 5

Our very own Emilee Knowlton, Ph.D., Senior Immunology Sales Specialist, will be speaking at this event about "An Integrated Approach to Managing Immunogenicity Risk and Optimum Protein Design".

ProImmune @proimmune.bsky.social · Mar 5

We're sponsoring #festofbiologics where science meets strategy!

Join us on April 23-24 in San Diego. From cutting-edge research to advancements in AI-driven drug discovery, this is where breakthroughs happen.

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ProImmune @proimmune.bsky.social · Mar 5

And here's the same #animalfree Ankyron™ specific to β-actin (red), lighting up the cytoskeleton that we highlighted for #FluorescenceFriday last week:

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ProImmune @proimmune.bsky.social · Mar 5

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social

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ProImmune @proimmune.bsky.social · Feb 28

Fab fine filaments of the cytoskeleton for #FluorescenceFriday lit up by our #animalfree Ankyron™ to β-actin (red), with nuclei counterstained with DAPI (blue) on our Echo Revolve #microscope @discoverecho.bsky.social An alternative counterstain to phalloidin! #immunology #3Rs @afruk.bsky.social

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ProImmune @proimmune.bsky.social · Feb 21

Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope
#bioscience #immunology #3Rs
@AFR_UK

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ProImmune @proimmune.bsky.social · Feb 14

Delicate red dendrites for #FluorescenceFriday - here our #animalfree Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope #immunology #3Rs #biomedical #research
@AFR_UK

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ProImmune @proimmune.bsky.social · Feb 7

It's February #FluorescenceFriday! Here's a beautiful image of #animalfree Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green)
@Discover_Echo

#immunology #cells #biomedical #3Rs
@AFR_UK

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ProImmune @proimmune.bsky.social · Jan 31

It's #FluorescenceFriday! We used our #animalfree antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve #microscope @Discover_Echo #immunology #3Rs

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ProImmune @proimmune.bsky.social · Jan 24

Banish January blues this #FluorescenceFriday and look into the lysosomes of Dendritic cells using our animal-free antibody replacement Ankyron™ binding LAMP3 (red), with nuclei counterstained with DAPI (blue). Imaging carried out with our @Discover_Echo #microscope. www.proimmune.com/ankyron/

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ProImmune @proimmune.bsky.social · Jan 23

📢 Join us next Thursday! 📢
The BSI Comparative and Veterinary Immunology Affinity Group (CVIG) is hosting an exciting online meeting. Discover the diverse applications of Ankyrons, which are next-generation binding reagents, in staining across species.
www.immunology.org/events/give-...

Immune responses investigated by spatial biology across species | British Society for Immunology

www.immunology.org