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Monika Wolkers @monikawolkers.bsky.social · 15h

#immunity #Tcells #mRNA #HuR #smFISH #RNAbiology

Monika Wolkers @monikawolkers.bsky.social · 15h

Funded by European Research Council (ERC)and Oncode Institute,
Research performed at Sanquin Blood Supply Foundation, in collaboration with the Vrije Universiteit Amsterdam (VU Amsterdam) Amsterdam

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Monika Wolkers @monikawolkers.bsky.social · 15h

She got great help for the data analysis from bioinformaticians (Philipp Savakis, Nikolina Sostaric, and Nandhini Kanagasabesan, and was helped by other labmembers Branka Popović, Leyma Wardak, Antonia Bradarić and Aurélie Guislain.

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Monika Wolkers @monikawolkers.bsky.social · 15h

This was a fantastic collaboration with the Evelina Tutucci lab who taught us all about smFISH in non-adherent cells. And a heroic effort of Maria Valeria Lattanzio, to dared to jump into the deep and who really pulled it off.

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Monika Wolkers @monikawolkers.bsky.social · 15h

We could simultaneously study how cytokine RNAs are transcribed and where mature mRNA goes to when T cells become activated.This method can shed light on as to why in some cases T cells can have really high mRNA levels, yet don't produce any protein (we showcase it for HuR deficiency).

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Monika Wolkers @monikawolkers.bsky.social · 15h

I am super proud that this paper is now out in EMBO Journal

lnkd.in/ev843k7P

What did we do? we optimized imaging of single molecules, in our case cytokine mRNAs with smFISH in primary human T cells.

This link will take you to a page that’s not on LinkedIn

lnkd.in

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Reposted by Monika Wolkers

Matthias Eberl @eberlmat.bsky.social · 12d

Somehow we don't talk about this enough.

Matthias Eberl @eberlmat.bsky.social · 15d

"Nigel meets tens of thousands of people on an annual basis."

Just amazing then how many of them are called Nathan Gill.

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Monika Wolkers @monikawolkers.bsky.social · 20d

Can we do the same with Farage please? Wilders? (who got a lot of platform by the journalists which in fact made him big... ) At least no more front page headlines. no place for lies there. Lets learn from this.

Monika Wolkers @monikawolkers.bsky.social · 20d

First cats in the US, and now Swans in the UK?! how absurd can it get...

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Monika Wolkers @monikawolkers.bsky.social · 23d

love it!

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Monika Wolkers @monikawolkers.bsky.social · Sep 3

our study on #gdTcells in pediatric neuroblastoma is out! In contrast to adult tumors, it is the gdTcells that not only expand, but that are the most tumor reactive #TILs.

#immunology #tumorimmunology

funded by KIKA, Sanquin and supported by Oncode

www.life-science-alliance.org/content/8/11...

γδ T cells are the prime antitumoral T cells in pediatric neuroblastoma

High-risk pediatric neuroblastoma patients have a dismal survival rate despite intensive treatment regimens. New treatment options are thus required. Even though HLA expression in neuroblastoma is low...

www.life-science-alliance.org

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Monika Wolkers @monikawolkers.bsky.social · Sep 3

our study on #gdTcells in pediatric neuroblastoma is now out in

Reposted by Monika Wolkers

DonnaDee 💙 @overallthiscrap.bsky.social · Aug 30

Unvaccinated citizens in the 50s

55 420 1.1K

Reposted by Monika Wolkers

Olga Nesterova @onestpress.onestnetwork.com · Aug 18

Kharkiv right now. Moscovia bombs sleeping families. 4 victims including kids.

Don’t tell me about peace that Putin wants. Do not dare.

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Reposted by Monika Wolkers

Ben Nicolet @bnicolet.bsky.social · Aug 1

Can you boost the protein expression in specific cell types?
That is what we asked in our study published a week ago in @scienceadvances
We used machine learning (AI) to learn the code for protein expression in immune cells.

www.science.org/doi/10.1126/...

Learning the sequence code of protein expression in human immune cells

SONAR informs the design of cell type–specific protein expression in human cells.

www.science.org

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Monika Wolkers @monikawolkers.bsky.social · Jul 29

#Tcells #RBPs #generegulation preprint

www.biorxiv.org/content/10.1...

Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells

Effective T cell responses against pathogens require a rapid yet tightly controlled remodeling of the proteome, and RNA binding proteins (RBPs) are key in this process. For instance, the RBP ZFP36L1 p...

www.biorxiv.org

Monika Wolkers @monikawolkers.bsky.social · Jul 29

👇New preprint:
Proximity labeling in human T cells uncovers ZFP36L1 as central hub for post-transcriptional gene regulation.
We found known and unknown ZFP36L1 interactors, their interaction dynamics and RNA (in)dependency, elucidating ZFP36L1's multifaceted role.

www.biorxiv.org/content/10.1...

Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells

Effective T cell responses against pathogens require a rapid yet tightly controlled remodeling of the proteome, and RNA binding proteins (RBPs) are key in this process. For instance, the RBP ZFP36L1 p...

www.biorxiv.org

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Monika Wolkers @monikawolkers.bsky.social · Jul 23

Great work spearheaded by Ben @bnicolet.bsky.social

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Monika Wolkers @monikawolkers.bsky.social · Jul 23

We used information on conservation, length, codon usage, miRNA and RNA Binding Protein and putative Post-translational modificationmotifs +more. Together #RNA abundance, we could predict protein abundance AND modulate protein expression with synthetic 3'UTRs, exemplified in #Tcells

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Monika Wolkers @monikawolkers.bsky.social · Jul 23

it is finally out! 👇 our #ML pipeline SONAR, which can predict the protein abundance in #immune cells. Using sequence features present in the #RNA. With @bnicolet.bsky.social

Supported by the @erc.europa.eu [email protected]

www.science.org/doi/epdf/10....

Learning the sequence code of protein expression in human immune cells

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www.science.org