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Metabarcoding and Metagenomics @mbmg.pensoft.net · 17m

Multiplex PCR sequencing represents a powerful and cost-effective tool for simultaneously detecting multiple aquatic invasive species in the early stages of invasion: doi.org/10.3897/mbmg...

Invasive species monitoring based on eDNA multiplex PCR sequencing

Invasive species pose a significant threat to global biodiversity and the stability of ecosystems. Although environmental DNA (eDNA)-based quantitative PCR is considered effective, its limited multipl...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · 20h

"Want to start using Nanopore technology to research protistan diversity? Check out our paper introducing a pipeline for creating OTUs from Nanopore metabarcodes — bridging the gap between short- and long-read metabarcoding." - Anna Karnkowska

doi.org/10.3897/mbmg...

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Metabarcoding and Metagenomics @mbmg.pensoft.net · 1d

Non-invasive swabbing of hive entrances is a highly effective biomonitoring method for collecting hive eNA and delivering comprehensive surveillance for animal, plant and microbial communities in the environment.

🔗 doi.org/10.3897/mbmg...

Surface swabs outperform most traditional honeybee (Apis mellifera) hive samples for recovery of eDNA and eRNA

Genetic sampling from European honeybee (Apis mellifera) hives is a promising repository of terrestrial environmental nucleic acids (eNA) from a range of taxonomic sources. eNA samples from honeybee h...

doi.org

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ARPHA Preprints @preprints.arphahub.com · 1d

A 16S rRNA dataset of Las Tablas de Daimiel National Park.

🔗 doi.org/10.3897/arph...

@mbmg.pensoft.net

A 16S rRNA dataset of Las Tablas de Daimiel National Park

Wetlands are ecosystems of environmental importance due to their role as biodiversity shelters. However, the intense anthropization of the landscape have propelled a decline in water quality and quant...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · 4d

Optimised eDNA and PCR-based metabarcoding significantly outperformed hybridisation capture and traditional field surveys for bryophyte detection in river ecosystems.

🔗 doi.org/10.3897/mbmg...

A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems

Despite their ecological importance, mosses remain under-represented in ecological studies due to their challenging detection in field surveys and morphological identification, exacerbated by the lack...

doi.org

Metabarcoding and Metagenomics @mbmg.pensoft.net · 6d

Researchers present the BaNaNA (Barcoding Nanopore Neat Annotator) pipeline and display the effectiveness of long-read Nanopore sequencing for protist biodiversity, ecology and evolution research.

🔗 doi.org/10.3897/mbmg...

@ankarn.bsky.social @fabnot.bsky.social

From short to long reads: enhanced protist diversity profiling via Nanopore metabarcoding

In the last decades, environmental metabarcoding has revolutionised biodiversity research, particularly for microbial organisms such as protists, enabling large-scale assessments of diversity and ecol...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · 11d

DNA metabarcoding identifies more invertebrate species under the Water Framework Directive than conventional methods, showing promise for faster and finer river health monitoring in Slovakia’s Carpathian streams.

🔗 doi.org/10.3897/mbmg...

Evaluating the potential of DNA metabarcoding for ecological status assessment under the Water Framework Directive: A case study on benthic invertebrates from Western Carpathian streams

Routine biomonitoring under the Water Framework Directive (WFD) follows a conventional methodology primarily based on the morphological identification of taxa within the five biological quality elemen...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · 14d

Her study identified key barriers to broader implementation, such as the need for international standards, better quantitative estimates and comprehensive reference libraries.

doi.org/10.3897/mbmg...

Technology Readiness Level of biodiversity monitoring with molecular methods – where are we on the road to routine implementation?

Human activities are causing rapid biodiversity loss across ecosystems, affecting human well-being and crucial ecosystem services. Traditional biodiversity monitoring tools cannot keep up with the inc...

doi.org

Metabarcoding and Metagenomics @mbmg.pensoft.net · 14d

Tiina Laamanen @tiinalaamanen.bsky.social cited her MBMG paper on molecular biodiversity monitoring methods as part of her #BGEconnects session on implementing #eDNA and other molecular monitoring methods in Finland!

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NeoBiota @neobiota.pensoft.net · 15d

After more than fourteen years of advancing research on biological invasions, we have published our 100th issue! 🌎

🔗 blog.pensoft.net/2025/09/29/a...

Advancing invasion science: NeoBiota publishes its 100th issue

The open-access journal NeoBiota has published its milestone 100th issue, celebrating more than fourteen years of advancing research in invasion science.

blog.pensoft.net

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Zoosystematics and Evolution @zse.pensoft.net · 19d

DNA barcoding reveals that the diversity of 𝑁𝑖𝑝ℎ𝑎𝑟𝑔𝑢𝑠 in France is dramatically underestimated.

🔗 doi.org/10.3897/zse....

Diversity of Niphargus (Crustacea, Amphipoda) in France, as revealed by DNA barcoding approach

Niphargus is the most speciose amphipod genus inhabiting groundwater of the Western Palearctic. The genus is renowned for its notorious taxonomy, associated with large within- but often negligible bet...

doi.org

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Reposted by Metabarcoding and Metagenomics

OBSGESSION Project @obsgession.eu · 25d

📰 A recent article, supported by our project, was published in Pensoft Publishers' (@pensoft.net) Metabarcoding and Metagenomics journal.

🧬 The study evaluates the TRLs of molecular tools for #monitoring like #eDNA and DNA #metabarcoding.

👀 Full article:
mbmg.pensoft.net/article/1308...

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Sep 11

The latest guest post on the @pensoft.net blog outlines a new, non-lethal method of studying pollinator networks. 🐝🧬

blog.pensoft.net/2025/09/11/t...

The bee’s knees: a new, non-lethal way to study pollinator networks

Researchers have created a new way to study pollinator networks, using metabarcoding and without killing bees, as described in this guest blog post.

blog.pensoft.net

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Sep 11

Read the original study here: doi.org/10.3897/mbmg...

Case study of non-lethal sampling for plant-pollinator networks via barcoding and metabarcoding on bumble bees in Germany

1. Insect decline is threatening ecosystem stability, making information about foraging preferences of pollinators a vital piece of information to acquire. A powerful emerging tool to study pollinator...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Sep 11

The latest guest post on the @pensoft.net blog outlines a new, non-lethal method of studying pollinator networks. 🐝🧬

blog.pensoft.net/2025/09/11/t...

The bee’s knees: a new, non-lethal way to study pollinator networks

Researchers have created a new way to study pollinator networks, using metabarcoding and without killing bees, as described in this guest blog post.

blog.pensoft.net

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Sep 2

Using molecular and morphological evidence, researchers present the first occurrences of the non-indigenous diatom species, 𝐷𝑖𝑠𝑐𝑜𝑠𝑡𝑒𝑙𝑙𝑎 𝑎𝑠𝑡𝑒𝑟𝑜𝑐𝑜𝑠𝑡𝑎𝑡𝑎 in the Danube River and the earliest occurrence in European freshwater ecosystems.

🔗 doi.org/10.3897/mbmg...

From Asia through America to Europe: Molecular and morphological evidence for a non-indigenous diatom species, Discostella asterocostata (B.Q.Lin, S.Q.Xie & S.X.Cai) Houk & Klee in Hungary and...

This study presents the first occurrences of the diatom species Discostella asterocostata in Europe’s second largest river, the Danube River and the earliest occurrence in European freshwater ecosyste...

doi.org

Reposted by Metabarcoding and Metagenomics

RIO (Research Ideas and Outcomes) journal @riojournal.com · Sep 2

🌎 Potential of simple, replicable methods to support long-term #monitoring demonstrated at a workshop of the #Marine #Biodiversity Observation Network Pole to Pole of the Americas that brought together participants from 10 countries earlier this year.

📑 Workshop Report: doi.org/10.3897/rio....

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Aug 29

@dominikbuchner.bsky.social
@leeselab.bsky.social

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Aug 29

By comparing three strategies for local bioassessments in streams using environmental DNA and RNA metabarcoding, researchers find the most effective strategy is to briefly incubate collected organisms in water then analyse the enriched eDNA.

🔗 doi.org/10.3897/mbmg...

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Reposted by Metabarcoding and Metagenomics

Nature Conservation @natureconservation.pensoft.net · Aug 21

Researchers utilise DNA metabarcoding to reveal distinct trophic niches among insectivorous bird species in central Peninsular Malaysia, highlighting the need to conserve insect-rich habitats within mixed-use landscapes.

🔗 doi.org/10.3897/natu...

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Reposted by Metabarcoding and Metagenomics

IMA Fungus @imafungus.pensoft.net · Aug 13

Combining culture-based approaches with molecular phylogenetics and eDNA metabarcoding, researchers uncovered two new fungal genera within the Lulworthiales, expanding the known ecological range of this order from marine to terrestrial saline habitats.

🔗 doi.org/10.3897/imaf...

From seagrass roots to saline soils: discovery of two new genera in Lulworthiales (Sordariomycetes) from osmotically stressed habitats

As part of an ongoing study of marine fungi associated with seagrasses, we discovered a novel root-fungus symbiosis in the Indo-Pacific species Thalassodendron ciliatum from Mauritius. Culturing its m...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Aug 1

Researchers demonstrate the effectiveness of DNA metabarcoding in assessing aquatic invertebrate diversity across an altitudinal gradient in the Tatra Mountains.

🔗 doi.org/10.3897/mbmg...

Gradient patterns in the Tatra Mountain lakes revisited with invertebrate DNA metabarcoding

Monitoring changes in freshwater fauna requires timely, efficient, and comprehensive data collection, needs that are increasingly met by molecular methods. This is especially important in remote regio...

doi.org

Metabarcoding and Metagenomics @mbmg.pensoft.net · Jul 30

COI barcoding is effective for species identification in 𝑃𝑎𝑐ℎ𝑦𝑔𝑟𝑎𝑝𝑠𝑢𝑠 crabs and in unravelling intraspecific patterns indicative of cryptic diversity guiding further taxonomic revisions integrating morphological, genetic and ecological data.

🔗 doi.org/10.3897/mbmg...

Mind the gap: DNA barcoding on Pachygrapsus crabs

DNA barcode databases for marine invertebrates faces significant challenges, with several species revealing taxonomic issues like misidentification, ambiguity or shared barcodes. This highlights the i...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Jul 21

Using light microscopy and metabarcoding, researchers have revealed microalgae diversity in yellowfever mosquito (Aedes aegypti) larvae guts and breeding sites in Thailand, providing potentially valuble information for biocontrol.

🔗 doi.org/10.3897/mbmg...

Microalgae diversity in Aedes aegypti larvae guts and breeding sites in Nakhon Si Thammarat, Thailand revealed by light microscopy and metabarcoding

Aedes aegypti poses a significant threat by transmitting diseases like dengue, chikungunya and Zika, leading to high morbidity and mortality worldwide. In recent years, the role of microalgae in mosqu...

doi.org

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Metabarcoding and Metagenomics @mbmg.pensoft.net · Jul 18

‪@microbesatsea.bsky.social

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