Lightnews — Scholar-powered news

A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context

1 1

Cell Biology J-Club @cellclub.bsky.social · 10h

A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context www.biorxiv.org/content/10.1...

Fluorescence microscopy has been widely used to reveal the spatial distribution of specifically labeled molecules, but it is blind to cellular context. Quantitative phase contrast microscopy (QPC) pro...

1 2

Cell Biology J-Club @cellclub.bsky.social · 10h

Genome-wide CRISPR Screen Identifies Sec31A as a Key Regulator of Alpha Cell Survival - Nature Communications

Pancreatic alpha cells resist stress better than beta cells, but the mechanisms underlying this observation are unclear. Here, the authors show that Sec31A regulates alpha but not beta cell survival u...

Cell Biology J-Club @cellclub.bsky.social · 10h

Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome - Nature Communications

K11/K48 branched ubiquitin chains regulate protein degradation and cell cycle progression. Here, the authors report the structural basis of how such a branched ubiquitin chain is recognized by the hum...

Cell Biology J-Club @cellclub.bsky.social · 10h

Of condensates and coats - reciprocal regulation of clathrin assembly and the growth of protein networks - Nature Communications

Biomolecular condensates of Eps15 recruit and assemble clathrin. In turn, clathrin assembly restricts condensate growth while imposing the preferred curvature of the clathrin lattice.

Ubiquilin-2 liquid droplets catalyze α-synuclein fibril formation | The EMBO Journal

1 2

Cell Biology J-Club @cellclub.bsky.social · 1d

Ubiquilin-2 liquid droplets catalyze α-synuclein fibril formation | The EMBO Journal www.embopress.org/doi/full/10....

imageimageFibril formation of α-synuclein is central to Parkinson’s disease (PD) pathology. This study shows that UBQLN2 droplets catalyze α-synuclein fibrillization via their liquid-gel/solid transit...

www.embopress.org

Cell Biology J-Club @cellclub.bsky.social · 1d

Chaperone-mediated autophagy regulates neuronal activity by sex-specific remodelling of the synaptic proteome
www.nature.com/articles/s41...

Client Challenge

Cell Biology J-Club @cellclub.bsky.social · 2d

Cholesterol sensing by the SCAP–FAM134B complex regulates ER-phagy and STING innate immunity
www.nature.com/articles/s41...

Client Challenge

Optogenetic control of PLC-γ1 activity polarizes cell motility

1 4

Cell Biology J-Club @cellclub.bsky.social · 3d

Optogenetic control of PLC-γ1 activity polarizes cell motility www.biorxiv.org/content/10.1...

Phospholipase C-γ1 (PLC-γ1) signaling is required for mesenchymal chemotaxis, but is it sufficient to bias motility? PLC-γ1 enzyme activity is basally autoinhibited, and light-controlled membrane recr...

Excitation Spectral Phasor Microscopy (ExSPM) unveils spatiotemporal heterogeneities in polarity of intracellular lipid droplets

Cell Biology J-Club @cellclub.bsky.social · 3d

Excitation Spectral Phasor Microscopy (ExSPM) unveils spatiotemporal heterogeneities in polarity of intracellular lipid droplets www.biorxiv.org/content/10.1...

Spatiotemporal heterogeneities in intracellular LD polarity are central to cellular homeostasis but remain largely unexplored. Here, we introduce Excitation Spectral Phasor Microscopy (ExSPM), which i...

Cell Biology J-Club @cellclub.bsky.social · 4d

Membrane protein condensates polymerize actin and form filopodia

Neuronal morphogenesis is guided by filopodia, dynamically generated plasma membrane protrusions filled with parallel actin filaments. However, how filopodial actin filaments are locally produced, org...

1 1

Cell Biology J-Club @cellclub.bsky.social · 4d

Combining FRET and super-resolution microscopy reveals kinase activation and mitochondrial activity at the nanoscale

Protein kinases are key regulators of intracellular signaling, showing activities in specific subcellular compartments and in micro- or nano-domains. Genetically encoded biosensors based on Förster′s ...

Cell Biology J-Club @cellclub.bsky.social · 5d

CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

Endosomes generate tubulo-vesicular carriers to redistribute proteins between plasma membrane, Golgi, and lysosomes. These transport routes employ distinct combinations of sorting nexins with complexe...

1 1

Cell Biology J-Club @cellclub.bsky.social · 5d

Molecular bases of the interactions of ATG16L1 with FIP200 and ATG8 family proteins - Nature Communications

ATG16L1 is a key autophagic protein. Here, the authors elucidate the molecular basis of ATG16L1 interactions with FIP200 and ATG8 family proteins, showing that the interaction of ATG16L1 with FIP200 is indispensable for the effective autophagic flux.

Ubiquitin-mediated mitophagy regulates the inheritance of mitochondrial DNA mutations

1 3

Cell Biology J-Club @cellclub.bsky.social · 6d

Ubiquitin-mediated mitophagy regulates the inheritance of mitochondrial DNA mutations | Science www.science.org/doi/10.1126/...

Mitochondrial synthesis of adenosine triphosphate is essential for eukaryotic life but is dependent on the cooperation of two genomes: nuclear and mitochondrial DNA (mtDNA). mtDNA mutates ~15 times as...

www.science.org

Cell Biology J-Club @cellclub.bsky.social · 6d

Optogenetic construction of de novo integrin-adhesion complexes reveals role for biocondensation in adhesion nucleation

Integrin-adhesion complexes (IACs) form spontaneously in cells on extracellular matrix substrates, allowing them to sense matrix composition and transduce force. However, IACs often do not form unifor...

ASPL couples the assembly of stress granules with their VCP-mediated disassembly

2 1

Cell Biology J-Club @cellclub.bsky.social · 6d

ASPL couples the assembly of stress granules with their VCP-mediated disassembly | Science Advances www.science.org/doi/10.1126/...

Disease relevant molecular interactions link RNP granule assembly with their active disassembly.

www.science.org

www.science.org/doi/10.1126/...

Cell Biology J-Club @cellclub.bsky.social · 6d

TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and Parkin-independent mitophagy

TRAF6 bridges innate immune signaling to mitophagy to promote glucose control during metabolic stress.

www.science.org

Cell Biology J-Club @cellclub.bsky.social · 6d

UBE2J2 sensitizes the ERAD ubiquitination cascade to changes in membrane lipid saturation - Nature Communications

ER membranes tune protein degradation to lipid composition. Using reconstitution approaches, the authors show that the ubiquitin conjugating enzyme UBE2J2 senses lipid packing, modulating its own and partner enzyme activities; together, they integrate lipid saturation and cholesterol signals.

The autophagy-specific exocyst subcomplex contributes to phagophore assembly site integrity by promoting phagophore expansion | PNAS

Cell Biology J-Club @cellclub.bsky.social · 7d

The autophagy-specific exocyst subcomplex contributes to phagophore assembly site integrity by promoting phagophore expansion | PNAS www.pnas.org/doi/10.1073/...

Autophagy is a crucial intracellular pathway for maintaining cellular homeostasis. It involves forming double-membrane vesicles called autophagosom...

www.pnas.org

1 3

Cell Biology J-Club @cellclub.bsky.social · 7d

Targeting myoferlin in ER/Golgi vesicle trafficking reprograms pancreatic cancer-associated fibroblasts | The EMBO Journal www.embopress.org/doi/full/10....

Targeting myoferlin in ER/Golgi vesicle trafficking reprograms pancreatic cancer-associated fibroblasts | The EMBO Journal

imageimageCancer-associated fibroblasts play key roles in the pancreatic tumor microenvironment, but their targeting has yet been unsuccessful. This work investigates intracellular trafficking of memb...

www.embopress.org

Cell Biology J-Club @cellclub.bsky.social · 7d

Analysis of native Ist1 dynamics reveals multiple pools of ESCRT-III on endosomes url: rupress.org/jcb/article-...

Analysis of native Ist1 dynamics reveals multiple pools of ESCRT-III on endosomes

This study defines the native dynamics of the ESCRT-III component Ist1 in mammalian cells, highlighting the existence of multiple, unique pools at endosome

rupress.org

2 5

Cell Biology J-Club @cellclub.bsky.social · 8d

CHIP, VCP, and Nucleolar Gatekeepers Remodel the Nucleolus into a Stress-Responsive Proteostasis Hub www.biorxiv.org/content/10.1...

CHIP, VCP, and Nucleolar Gatekeepers Remodel the Nucleolus into a Stress-Responsive Proteostasis Hub

The nucleolus, classically dedicated to ribosome biogenesis, also acts as a stress-responsive proteostasis hub. During heat shock, misfolded proteins accumulate in its granular component (GC), but whe...

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates

Cell Biology J-Club @cellclub.bsky.social · 8d

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates www.biorxiv.org/content/10.1...

The endoplasmic reticulum (ER)'s continuous morphology is tightly controlled by ER-shaping proteins, whose genetic or expression defects drive a spectrum of neurodegenerative disorders from Hereditary...

MBOAT7 and CDS2 coordinate lipid droplet dynamics and cholesterol metabolism in liver

Cell Biology J-Club @cellclub.bsky.social · 9d

MBOAT7 and CDS2 coordinate lipid droplet dynamics and cholesterol metabolism in liver www.biorxiv.org/content/10.1...

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent disorder worldwide characterized by the accumulation of hepatic lipid droplets (LDs). However, the mechanisms by ...