Mutator phenotypes are short-lived due to the rapid accumulation of deleterious mutations. Yet, recent observations reveal that certain fungi can undergo prolonged accelerated evolution after losing genes involved in DNA repair. Here, we surveyed 1,154 yeast genomes representing nearly all known yea …

Cistanche deserticola is a holoparasitic plant of the Orobanchaceae family, parasitizing the roots of Haloxylon ammodendron. The absence of a high-quality genome has impeded understanding of C. deserticola's parasitic mechanisms. We present a chromosome-level assembly of C. deserticola (6.26 Gb) usi …

Inherited biallelic mutations in the CLN7 gene result in the variant late infantile onset neuronal ceroid lipofuscinosis, a subtype of Batten disease (BD), a severe and fatal childhood neurodegenerative disease. Intriguingly, CLN7 genetic variants have also been associated with retinopathies, amyotr …

Salmonella Derby (S. Derby) is transmitted to humans through contaminated pork products. S. Derby ST40, with multidrug resistance (MDR) and extensive drug resistance, poses major food safety and public health challenges. By integrating antimicrobial susceptibility testing, phylogenetic analysis, phy …

This dataset offers new insights into the microbial communities inhabiting the conifer needle surface, laying the foundation for future research on needle microbiomes across temporal and spatial scales. Variation in functional capabilities, such as volatile organic compound (VOC) degradation and pol …

Bacterial extracellular vesicles (BEVs) are nanoscale membrane-bound structures secreted by prokaryotic cells and have recently gained considerable attention in environmental pollution research. By encapsulating virulence factors and antibiotic resistance genes (ARGs), BEVs can persist in aquatic, s …

The global rise of antimicrobial resistance (AMR), exacerbated by the COVID-19 pandemic, has led to a surge in infections caused by multidrug-resistant (MDR) bacteria. A key driver of this phenomenon is co-selection, where exposure to one antimicrobial promotes resistance to others via horizontal ge …

Seen through a One Health perspective, plasmids act as global links, connecting human, animal, and environmental microbiomes while broadening the ecological scope of resistance and virulence. By combining knowledge about plasmid classification, mobility, resistance, virulence, and data sources, this …

<span><i>Pseudomonas aeruginosa</i> is an opportunistic pathogen of public health concern. This study aimed to investigate the prevalence of <i>P. aeruginosa</i>, some virulence factors, and antimicrobial resistance patterns and highlight the potential pathways of horizontal <i>bla</i>_SHV-resis</span> …

Antimicrobial resistance (AMR) is widely recognized as a major global public health threat, yet its origins and implications extend beyond the simple misuse or overuse of antibiotics. This study explores AMR as a complex, multifactorial phenomenon shaped by biological, clinical, dental, environmenta …

Plasmid-mediated transmission can account for half of carbapenem-producing Enterobacterales (CPE) dissemination, underscoring the need to identify genetic determinants of plasmid persistence in the hospital setting. From 1,088 CPE isolates detected through nationwide surveillance in Singapore over f …

Many invertebrates harbor the vertically transmitted endosymbiotic bacterium Cardinium hertigii, and some display altered reproductive phenotypes due to manipulation by Cardinium. Despite their host impact, genomic information for reproductive manipulator strains of Cardinium is sparse. Of the three …

The relentless global proliferation of antibiotic resistance genes (ARGs) poses a profound threat to public health and ecological stability. Unlike static chemical pollutants, ARGs propagate through horizontal gene transfer (HGT)a dynamic biological process that facilitates the cross-taxa dissemina …

The mitochondrial genome of 'Zhuyeqi' consists of one circular chromosome and six linear chromosomes, with a total length of 911,255 bp and a GC content of 46%. Genome annotation identified 77 functional genes, including 38 protein-coding genes (PCGs). The study revealed heterogeneously distributed …

We identified 1790 AmpC enzymes in nine distinct groups across ESKAPE pathogens, with species-specific distribution patterns and notable absence in Gram-positive bacteria. The PIB enzyme group demonstrated unique motif variants (YST/AQG) conferring carbapenem resistance, while other groups maintaine …

This study emphasizes the critical role of wastewater treatment plants (WWTPs) in facilitating the horizontal transfer of ARGs through biosolids. As biosolids are routinely applied to agricultural soils, their load of clinically relevant ARG content and transferability pose risks to animal and human …

A total of 73 <i>Enterobacteriaceae</i> strains were isolated, including <i>Klebsiella pneumoniae</i>, Salmonella enterica, <i>Enterobacter hormaechei</i>, and <i>Escherichia coli</i>. Resistance rates to tetracycline, ciprofloxacin, nalidixic acid, sulfamethoxazole-trimethoprim, and ampicillin exce …

Our findings indicate that ticks play a significant role in the transmission and evolution of poxviruses. These viruses demonstrate the capacity to modulate virulence and adaptability through horizontal gene transfer, gene recombination, and gene mutations, thereby promoting co-existence and co-evol …

While resequencing <i>Microcystis aeruginosa</i> (PCC7806) and its nontoxigenic mutant (PCC7806 Δ<i>mcyB</i>), we discovered identical unreported plasmids in both strains. These strains were separated in culture over 25 years ago, resulting in sequence divergence among their chromosomes. RNA-seq dat …

As a significant group of agricultural and forestry pests, Coccomorpha warrants in-depth investigation into their environmental adaptation mechanisms. This study conducted a comparative genomic analysis using five published chromosome-level genomes of Coccomorpha species. Phylogenetic analysis revea …

Antimicrobial resistance (AMR) is a critical global challenge, yet the role of environmental dissemination of antibiotic-resistant bacteria remains underexplored, particularly in developing regions. This study investigated urban wastewater overflows from public streets as vectors for extended-spectr …

The transition from autotrophy to heterotrophy in parasitic plants disrupts organellar coordination and presents a unique opportunity to examine the coevolution of cellular genomes. Using the Broomrape family (Orobanchaceae) as a model, we analyzed mitochondrial and plastid genome evolution across 3 …

The clonal evolution model provides a framework for understanding the evolution of cancer cells. According to this model, cancer cells accumulate genetic mutations over time, and these mutations are passed down to their descendants, leading to genetic diversity within the tumor. Some of these mutati …

This study confirms that antibiotic use in goat farming significantly influences the development of ABR in <i>E. coli</i>. The detection of resistance in farms without antibiotic use underscores the need to investigate other contributing factors, such as environmental residues and horizontal gene tr …

Multidrug-resistant bacterial infections are a major global threat, exacerbated by globalization and poor sanitation. Bacteria develop resistance through mechanisms like enzymatic degradation, efflux pumps, and horizontal gene transfer. Rapid diagnostics and artificial intelligence are crucial for o …